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Cell culture Related Discussions

1. productivity - erythropetin , glycosylation and cell count (reply: 1)
   
2. cell lysate colorless? - (reply: 1)
   
3. How to prevent cell aggregation - (reply: 1)
   
4. Does anyone else gas their cells like this?! - (reply: 5)
   
5. Problem with CD2F1 mouse CT26 cell cachexia protocol. - CT26 treated mice gained weight. (reply: 2)
   
6. Transient transfection trouble- massive cell death - (reply: 1)
   
7. can i use antibiotics with lipofectamine - my cells have antibiotic as selective marker and need neomycin (reply: 4)
   
8. animal cell lysis, on plates - (reply: 2)
   
9. Sp2/o Myeloma cell line - Media for growing sp2/0-Ag 14 cell line (reply: 4)
   
10. selection of transfected cells - (reply: 1)
    
11. Apoptosis by FACS - How to gate the cells by using Apoptosis kit from BD (reply: 1)
    
12. Stable transfection problem - Problem with pBM vector and Phoenix packaging cells (reply: 3)
    
13. Should i use cells healed from mycoplasma - (reply: 2)
    
14. How to count cells on plate - (reply: 3)
    
15. What's the major cell type in thymus - (reply: 1)
    
16. Transformation of non-competent E. coli? - Any shortcut for preapring competent cells? (reply: 2)
    
17. How to change medium without dislodging the cells - (reply: 1)
    
18. boosting expression in stable cell line? - (reply: 5)
    
19. Cell Lysis - (reply: 2)
    
20. too few cells used for electroporation/ transfection - (reply: 4)
    
21. Serum Deprivation - MDA-MB-231 cells - (reply: 5)
    
22. any way to inactivate trypsin without using FBS? - (and still let cells survive ^__^) (reply: 3)
    
23. VEGF secretion in MDA-MB-231 cell line - (reply: 3)
    
24. Quantification of VEGF peptide levels in human breast cancer cells. HELP! - (reply: 6)
    
25. THP-1 cells and 2-mercaptoethanol - (reply: 1)
    
26. making Stocks of HL 60 cells - making Stocks of HL 60 cells (reply: 1)
    
27. Exogenous protein expression - Problems in protein expression mammalian cells (reply: 2)
    
28. Mycoplasma contamination. - (reply: 6)
    
29. What do cells look like after nuclear extraction swell/break step? - (reply: 4)
    
30. cell and tissue culture - healthy test (reply: 1)
    
31. cell migration assay - (reply: 2)
    
32. Need human cells for a science fair project - (reply: 4)
    
33. How to inhibit trypsin? - (reply: 3)
    
34. THP-1 cell culture - (reply: 3)
    
35. What kind of cells is it? - need which counterstain? (reply: 1)
    
36. Mammalian expression problem - HEK cells expression (reply: 3)
    
37. Cell pellet after freezing - (reply: 3)
    
38. Contamination and its fungus - persistant fungal contamination in cell cultures (reply: 1)
    
39. cell culture maintainance - (reply: 3)
    
40. Removing all cell surface proteins/receptors - (reply: 3)
    
41. pls recommend a serum independent expression promoter for mammalian cells - (reply: 1)
    
42. The action of alcohol on bacteria cells - Washing bacteria cell (resuspending) pellets with methanol? (reply: 2)
    
43. Spontaneous Cell lysis after IPTG induction - (reply: 4)
    
44. PBS for releasing anchored cells, what kind? - (reply: 2)
    
45. Treating cells with cytokines - Should you take out growth factors before treating? (reply: 2)
    
46. Which part of the kidney are COS-7 cells from? - (reply: 1)
    
47. Ways to stop the cell at G1 phase? - (reply: 2)
    
48. Chemical transformation in 96 well MTP - How to make competent cells in MTPs (reply: 2)
    
49. Immunstaining cells fixed in 10% formalin- possible? - (reply: 8)
    
50. Do COS-7, CHO-K1 and LLC-PK1 cells express dopamine receptors? - I need an answer soon... (reply: 2)
    
51. difference between transformed and immortalized cells - (reply: 3)
    
52. Is it hard to make knock-out cells/mice? - (reply: 4)
    
53. How many O.D. cells to load onto SDS-PAGE? - (reply: 2)
    
54. trypsin - wat is the source of trypsin we use in cell culture (reply: 1)
    
55. Troubles in IL-8 measurement of tissue cells cultured with E.coli - (reply: 1)
    
56. Competent Cells - (reply: 1)
    
57. Mioblasts primary cell culture - counting the number of myoblasts (reply: 1)
    
58. PC12 cells do not attach on collagen - (reply: 4)
    
59. RNA extraction from frozen cells suspended in PBS - (reply: 2)
    
60. cell fractionation and low protein concentration in cytosol - (reply: 1)
    
61. Cell Clumping Problem - Cells Stick to Eachother and Not the Flask (reply: 2)
    
62. IFA of cell surface markers - IFA of osteoclasts not working, help! (reply: 2)
    
63. Linear DNA transfection - Daudi cells (reply: 3)
    
64. Adding some PBS to cells - (reply: 3)
    
65. Dead cell staining - what kind of dye? (reply: 5)
    
66. Quantifying Cell Lysis or the Presence of DNA - (reply: 3)
    
67. Trypan Blue and fixed cells - Help settle our lab argument (reply: 7)
    
68. What exactly are CD4+3- inducer cells? - simply a specific form of "T-helper-cells"? (reply: 2)
    
69. Confirmation of the demethlayting treatment in cells - (reply: 1)
    
70. In vitro toxicology test by animal cell line - Looking for methods (reply: 1)
    
71. RNA Extaction from microglial cells culture - the best method for RNA extraction of microglial cells culture (reply: 2)
    
72. inoculating liquid media from frozen cell stock ok? - (reply: 6)
    
73. How to deal with so many false positive clones - Transfer linear DNA into E.coli competent cells... (reply: 2)
    
74. Cell treating - Do you... (reply: 4)
    
75. Do I need to count cells for PI staining? - (reply: 1)
    
76. cell lysis - (reply: 3)
    
77. How to normalize ELISA measurements? - Total protein, cell number, or DNA? (reply: 1)
    
78. How to fixate cells onto glass microscope slide - (reply: 2)
    
79. Should I chuck my competent cells? probably contaminated.. - (reply: 4)
    
80. Is there any destructive effect on nucleic acid when harvest the cell by scrapin - (reply: 1)
    
81. Failure in cell detachment after trypsinization - (reply: 9)
    
82. Transfected copies per cell - (reply: 1)
    
83. please help me identify if I have cell culture contamination =( - (reply: 1)
    
84. RAW 264 cells - (reply: 1)
    
85. How to make a growth curve of Raji cell - (reply: 1)
    
86. How to concentrate retrovirus produced by Phoenix-Ampho cell - (reply: 4)
    
87. Cell Counting Reproducibility - (reply: 1)
    
88. Have I killed my cells? Doh! - (reply: 3)
    
89. microplate for cell culture? - (reply: 1)
    
90. Looking for specific cancer cell line - Need a parent line with metastatic and non-metastatic lines (reply: 2)
    
91. NF-kB binding activity in neutrophils and mononuclear cells - (reply: 1)
    
92. Adenoviral purification by CsCl - Only cell pellet or supernatant too??? (reply: 5)
    
93. Cancer stem cells in cell lines? - (reply: 1)
    
94. mesenchymal stem cell differentiation into osteoblasts (alizarin) - (reply: 2)
    
95. Dendritic cell growth medium - (reply: 3)
    
96. Troubles in transfection methods! - All cells die before making a selection (reply: 5)
    
97. DsRed stable transfections? - problems with Stoplight vector in Flip-in cells (reply: 4)
    
98. siRNA into RAW264.7 cells? - any ideas (reply: 4)
    
99. Boyden chamber - isolation of cells? - (reply: 3)
    
100. Heat-shock treatment problems with adherent cells - (reply: 1)
     
101. identification of cell culture contamination - (reply: 1)
     
102. No Retrovirus using the Phoenix - Ampho cells - (reply: 1)
     
103. Cell death after electroporation - (reply: 1)
     
104. IEC-6, IL-6 and LPS - Cannot get my IEC-6 cells to secrete Il-6 (reply: 2)
     
105. cell lysis buffer to use directly on the dish - (reply: 1)
     
106. Creating stable cell lines - (reply: 4)
     
107. I need help for my Raji cell culture - (reply: 2)
     
108. Cholesterol Assay - Commerically available cholesterol assay kit for cell extracts (reply: 1)
     
109. Help on transfecting MCF7 and HCT 116 cells - (reply: 3)
     
110. Can Osteosarcoma cells change their phenotype? - previously "Name this cancer cell's purpose and parts" (reply: 1)
     
111. Cell stats - looking for info (reply: 1)
     
112. Apoptosis vurses necrosis - Way to find wether the cell death is due to apoptosis or necrosis? (reply: 13)
     
113. Storage of supernatant that contain proteins after cell lysis. - (reply: 7)
     
114. cell culture question - passage number - (reply: 5)
     
115. complete cell lysis - I want to know what to use to completely lyse/destroy the cells (reply: 1)
     
116. A problem with transient expression a myc-tagged protein in 293T cells - (reply: 1)
     
117. when can I start perform experiments on cells? - (reply: 6)
     
118. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 8)
     
119. Cell surface Protein Isolation - Protein extraction procedure (reply: 4)
     
120. 293t Cell Attachment Issues - (reply: 7)
     
121. Name this cancer cell's purpose and parts - (reply: 1)
     
122. giant cells? - (reply: 6)
     
123. Washing cells with...? - (reply: 8)
     
124. Direct Cell Lysis in Laemmli - (reply: 1)
     
125. cell culure - need your help (reply: 5)
     
126. Cell Morphology - (reply: 1)
     
127. Tearing and Peeling of Cell Monolayers in Flasks - Advice please? (reply: 2)
     
128. When to use PMSF in a cell lysis buffer? - (reply: 9)
     
129. how to transport a culture cell 35 mm plate or 25cm2 flask - confluence is very very slow (reply: 3)
     
130. staining for receptors in cell line - (reply: 1)
     
131. Huh7 cell line growth problem - cells are not growing well (reply: 1)
     
132. Counting B cells in hemacytometer - (reply: 2)
     
133. Cells do not attach in the flasks - (reply: 11)
     
134. Coating cell dishes with peptides - (reply: 2)
     
135. ATDC5 CELLS CULTURE PROBLEMS! - (reply: 3)
     
136. Does it look like cell clumps/artefact/contamination? - (reply: 3)
     
137. Apoptosis in adherent and floating cells - (reply: 1)
     
138. What would happen if we don't use protease inhibitors? - for cell lysis by RIPA, NP40 or any other lysis buffer (reply: 8)
     
139. Starting a cell line - (reply: 6)
     
140. what cell line to use for visualizing membrane localized protein - (reply: 1)
     
141. what cell line to use for visualizing membrane localized protein - (reply: 1)
     
142. Define cell passage number - (reply: 2)
     
143. newly purchased cells from ATCC and they dont grow :-( - (reply: 3)
     
144. THP-1 cells from Sigma - (reply: 1)
     
145. preserving THP-1 cells - (reply: 1)
     
146. DNA "snot" in cell lysis/protein extraction - (reply: 6)
     
147. cell proliferation - (reply: 4)
     
148. DNA extraction from dry cells - How to extract DNA from dry cells (reply: 9)
     
149. Is fat cell looked white after HE staining? - (reply: 10)
     
150. H.influenzae and S.pneumoniae cell culture - H.influenzae and S.pneumoniae grow on plates but not in broth (reply: 1)
     
151. What does abbreviation +ve mean? - e.g. CD45+ve cells (reply: 8)
     
152. Cells die after changing medium - Is there role of FBS? (reply: 1)
     
153. Cell line died; unknown cause... - (reply: 11)
     
154. Different cell culture plastics, problems with cell adherance - (reply: 6)
     
155. My clones disappear after subculturing! - (reply: 3)
     
156. transfecting large gene in mammalian cells - difficulties in transfecting large gene in mammalian cells (reply: 5)
     
157. Cytotoxicity assay with RAW 264.6 cells - (reply: 1)
     
158. Comparing surface protein expression of different cell lines - (reply: 1)
     
159. Decide which cell line to use - (reply: 3)
     
160. How to compare the fluorescence staining signal in cells - (reply: 6)
     
161. Cell seeding density - (reply: 11)
     
162. what cells to use for expression of pGEX3? - (reply: 1)
     
163. 293T cells adhesion and transfection... - Looking for cell line for localization studies... (reply: 3)
     
164. What's "polarized T cell"? - (reply: 1)
     
165. Cell culture supernatant - Is it just the media from cultured cells? (reply: 1)
     
166. SMP30 silencing in vivo - stem cell biology (reply: 2)
     
167. growth curve? - how to establish growth curve of specific cell line.... (reply: 1)
     
168. Cell Lysis buffer....do I have to add sodium phosphate to the buffer? - lysis buffer for DNA extractions (reply: 4)
     
169. transfecting human lymphoblastoid cells - (reply: 2)
     
170. p53 status in cell lines - (reply: 3)
     
171. H9 Cell transfection - H9 cell transfection protocol (reply: 4)
     
172. Cell Adherence - (reply: 2)
     
173. Cotransfection of DNA and siRNA in Neuro2a cells - (reply: 1)
     
174. How to kill bacteria in treated cells? - (reply: 6)
     
175. where can I get hmec-1 cell line? - where can I get hmec-1 cell line? (reply: 1)
     
176. cell line to use for neural regeneration? - (reply: 1)
     
177. cell culture - (reply: 1)
     
178. Does immortalization affect non-dividing short-living cells? - (reply: 3)
     
179. transporting cell lines on board of international airflight - how? (reply: 4)
     
180. Why do viable cells take up DAPI? - DAPI must only stain non-viable cells (ICC) (reply: 7)
     
181. ng into competents cells - (reply: 2)
     
182. Quenching oxygen in cell culture - (reply: 1)
     
183. trypsinization in what temperature will disturb less the cells? - (reply: 6)
     
184. what is trypsin assay by BAEE - (reply: 2)
     
185. Inhibitors in cell culture... - DMSO???? (reply: 4)
     
186. Are these cells still viable? (Trypan Blue Stain) - Using this stain for the first time against Sf9 cells (reply: 6)
     
187. % Cytotoxicity or Relative Cell Damage less than zero - What does it imply? (reply: 4)
     
188. Trypsin Assay Calculation - Please Help me!!Really in urgent (reply: 4)
     
189. 3T3-L1 cells peeling/curling when differentiated - any help? - (reply: 6)
     
190. Ratio between cells and lysis buffer - (reply: 1)
     
191. cell culture - (reply: 1)
     
192. Immunofluorescense of floating cells - How to IF the floating cells during apoptosis? (reply: 4)
     
193. Nocodazole, cell cycle arrest in G2/M - (reply: 3)
     
194. What cell lines can be used to run an anti-angiogenic assays? - (reply: 1)
     
195. how to maintain iPS cells - (reply: 2)
     
196. NK Cell Isolation - negative selection kits - (reply: 1)
     
197. xenografts analysis of GFP-expressing tumor cells? - (reply: 2)
     
198. Cell count in solution - (reply: 2)
     
199. Lysing cells with RIPA containing SDS - Stickiness of the lysate (reply: 5)
     
200. Using TrypLE as alternative for trypsin - in cell isolation from tissues (reply: 3)
     
201. IL4 and GMSCF for DC culture - how much IL4 and GMSCF for culturing dendritic cells (reply: 2)
     
202. Cell Culture protocol by Current Protocol - free online version from invitrogen (reply: 3)
     
203. cell culture protocol (URGENT) - (reply: 1)
     
204. cell cryopreservation in -80 - (reply: 2)
     
205. PMA Ionomycin Cell Culture / Dilution and Math Check - (reply: 1)
     
206. Cell culture being poisoined for nearly a year - Done by unknown person(s) (reply: 33)
     
207. Zymolyase treatment of yeast cell - (reply: 1)
     
208. Cell growth inhibition by lipofectamine 2000 - (reply: 1)
     
209. Immortalize mouse T cells in vitro - (reply: 1)
     
210. T cell activation for intracellular cytokine detection by Flow Cytometry - (reply: 1)
     
211. Trypsin concentration and preparation - How to dissolve Trypsin powder? (reply: 2)
     
212. Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
     
213. Stem Cell Niche - Murine Adult hematopoietic stem cell division Niche?? (reply: 1)
     
214. Cell death after PMA treatment - (reply: 5)
     
215. spinner flasks cell maintenance - (reply: 3)
     
216. what protease is best for primary cell culture - (reply: 1)
     
217. contamination of DH5alpha cell line - (reply: 6)
     
218. Basic immuno/histo common knowledge question - Where can I find what cells in what organ? (reply: 4)
     
219. MDCK cell experience - (reply: 2)
     
220. Help MCF7 , MDA231 cells - Treatment of cells. (reply: 1)
     
221. Expression of EGFR in MDA-MB-435 cell line - (reply: 2)
     
222. How does invitro morphology change w/cell cycle - (reply: 1)
     
223. how to distinguish monocyte and dendritic cell under microscope - (reply: 2)
     
224. protein extraction from mammalian cell line - preferred protocol? - (reply: 2)
     
225. cell cultur medium - (reply: 3)
     
226. DNA extraction...help needed! - extracting dna from buccal cells in ethanol (i think) (reply: 5)
     
227. High-throughput screening of suspension cell lines - (reply: 2)
     
228. Cell Clumping - (reply: 1)
     
229. Single Bacterial Cell Isolation for Comet Assay - (reply: 1)
     
230. Help with clonogenic survival assay for PC3 prostate cancer cell line - HELP! (reply: 5)
     
231. FACS data analysis - understanding cell quest program and reading data from it (reply: 1)
     
232. Competent cells for transformation - (reply: 1)
     
233. Problems with cell lysis - (reply: 3)
     
234. How to induce expression in mammalian cells with the pcDNA/V5/GW vector ? - (reply: 3)
     
235. Causes for vacuoles or bubbles in cultured cells - (reply: 7)
     
236. Mycoplasma - How to detect and treat mycoplasma contamination in culture and RNA? (reply: 9)
     
237. In need of advice on HUVEC cell culturing - (reply: 18)
     
238. Post-doc position available in my lab - Leukemia stem cells (reply: 4)
     
239. what are the methods available for protein extraction from cell line? - (reply: 6)
     
240. Is it a general effect that stably transfected cell line has lower cell prolifer - (reply: 4)
     
241. Cell Counting in Hemocytometer - Teach me short cut to count in this chamber (reply: 13)
     
242. how can i seed 0.5 cell /well? - (reply: 10)
     
243. Best method to locate a receptor on breast cancer cell? - Using an AFM (reply: 2)
     
244. RPM for Pelleting down cells - (reply: 3)
     
245. the latest research trend in dendritic cells - the heat research topics related to dendritic cells (reply: 1)
     
246. how to culture cancer stem cells? - help (reply: 1)
     
247. T cell proliferation assay with macrophages - (reply: 2)
     
248. Staining suspension cells for microscopy - (reply: 1)
     
249. Problems when harvesting cells - (reply: 4)
     
250. Liver cancer primary cell culture - urgent!Thanks (reply: 1)
     
251. About red precipitate during passing the cell line - (reply: 2)
     
252. Umbilical cord blood stem cells - (reply: 1)
     
253. housekeeping gene for B cell lines - (reply: 1)
     
254. Cell lysate preparation - (reply: 2)
     
255. Who uses non-adherent cells? - like NCI-H69... are they ready to use after passing? (reply: 1)
     
256. Cell ELISA, Permeabilization cell - (reply: 1)
     
257. no pellet after centrifugation for making competent cells - (reply: 4)
     
258. Cell cycle analysis. - Cell synchronized or no-synchronized. (reply: 6)
     
259. Daudi cells transfection - (reply: 1)
     
260. express toxic protein in insect cells - (reply: 2)
     
261. Primary tracheal cell culture - (reply: 1)
     
262. Any commercially available primary cells? - Need some cardiomyocytes (reply: 1)
     
263. Need some advice on HeLa cells health hazards - (reply: 7)
     
264. two bacteria populations observed when there should be just one - how to get rid of cell doublets? (reply: 4)
     
265. Reviving THP1 cells - (reply: 6)
     
266. help with making ultra competent cell (Inoue method) - (reply: 3)
     
267. Protein Isolation of very low cell number... - (reply: 4)
     
268. How to seed less number of cells - (reply: 2)
     
269. making INV110 competent cells - (reply: 1)
     
270. "Twin-peak" @ G2/M phase? - funny result of cell cycle assay (reply: 1)
     
271. b-mercaptoethanol and stem cells - (reply: 3)
     
272. Glioma cell lines - (reply: 2)
     
273. Cell fractionation problem - Nucleaus forms a pellet that can't be suspended (reply: 5)
     
274. Storage of Cell pellet - (reply: 8)
     
275. Bradford problem in cell lysates - (reply: 1)
     
276. Intracellular proteins on live cells - (reply: 3)
     
277. Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
     
278. making a stable cell line - (reply: 3)
     
279. Medium for culturing 2 types of cell together - (reply: 2)
     
280. siRNA transfection in MLE12 cells - (reply: 3)
     
281. Transfect competent cell? - (reply: 1)
     
282. Problem in growing DG44 CHO cells - (reply: 2)
     
283. Need advice on counting low cell numbers - (reply: 1)
     
284. making stable cell line - (reply: 5)
     
285. Can I load 1N NaOH in cell culture plate? - 1N NaOH is strong alkaline, will it eat up the glass? (reply: 4)
     
286. GFP tagged bacterial Cell preparation for FACS analysis - (reply: 2)
     
287. Why 10% serum for cell culture? - (reply: 11)
     
288. Cell culture basic questions - Basic questions on cell culture (reply: 7)
     
289. cell based system - (reply: 1)
     
290. flourescence in non-transfected cells! - (reply: 4)
     
291. Seeding cells on cover slips - how to get my cells to grow confluently (reply: 5)
     
292. % confluency question - when to transfect the cells? (reply: 2)
     
293. Dead cell staining on fixed cells - (reply: 1)
     
294. Leakage of ER(T2)-fusion protein ? - in cell culture (reply: 2)
     
295. The best way for RNA extraction from cells in RNAlater? - TRI Reagent or RNeasy or something else? (reply: 1)
     
296. Free grid image for counting cells (freeware) - simple but useful (reply: 7)
     
297. Question on cell line - (reply: 1)
     
298. Cell toxicity and color - (reply: 1)
     
299. T98G cell size - Coulter counter (reply: 1)
     
300. PCR and competent cells - (reply: 3)
     
301. TOP10 competent cell with another vector - (reply: 1)
     
302. Nuclear extracts from myeloid cells? - (reply: 9)
     
303. Suddenly no colonies, can cells 'go off' in -80? - (reply: 1)
     
304. primary Hepatocytes - experts advice - (reply: 1)
     
305. Plating cells in Serum-free medium - How to plate Mcf7 cells in a serum free medium? (reply: 7)
     
306. siRNA for retinal pigment epithelial cells - (reply: 1)
     
307. Overshot pH of Competent cell buffer (RbCl) :( - (reply: 3)
     
308. high levles of cell death in PC12 cells - (reply: 3)
     
309. Jurkat T cell imaginge - (reply: 1)
     
310. PMA - used to differentiate THP-1 cells - (reply: 6)
     
311. How much pellet do you get after using RIPA? - How long to keep cells in RIPA? (reply: 4)
     
312. Help...cell transfected with empty vector(control) don't retain fluorescence - PIRES2 (reply: 3)
     
313. HOW TO MAKE A STABLE TRANSFECTED CELL LINE with COS-7? - (reply: 2)
     
314. tranwells and C6 glioblastoma cell migration - (reply: 1)
     
315. High autofluorescence in cultured cells - (reply: 8)
     
316. Cell culture contamination - (reply: 9)
     
317. Primary bone cells? where to buy - (reply: 4)
     
318. yeast cell cycle and temperature - FACS profile at 25 and 36 (reply: 1)
     
319. cells culture - (reply: 6)
     
320. Melanin extraction from melanoma cells, How? - (reply: 3)
     
321. Western blot is not working with stable cell line=please help - no expression of my protein detected (reply: 5)
     
322. Strange black particles while growing Sf9 insect cells - (reply: 3)
     
323. PC12 cells are not "round up" - (reply: 2)
     
324. I thawed my primary cells and froze them again? serious mistake? - cell culture (reply: 5)
     
325. cell death after catalase overexpression - (reply: 2)
     
326. FRET Imaging :Hela cells - (reply: 2)
     
327. GFP Tobacco BY-2 cell sorting - (reply: 1)
     
328. Jurkat Cells and Proliferation Assay - Click-iT EdU - (reply: 2)
     
329. Detection of GFP expressing cells - fix or not? - (reply: 3)
     
330. cell density - (reply: 2)
     
331. Cell culture unidentifiable 'contaminant' - weird things appearing in my culture (reply: 1)
     
332. 3-D tissue model for endothelial cell culture - Looking for any in vitro 3-D models to grow endothelial cells to measu (reply: 1)
     
333. Cell harvest - Cell harvest (reply: 1)
     
334. How to kill yeast cells :) - chloramine or autoclaving (reply: 19)
     
335. Propagation of breast cancer cell lines in serum free media? - (reply: 1)
     
336. RNA isolation from small amount of suspension cells - (reply: 3)
     
337. Subculturing without trypsin - (reply: 2)
     
338. Culturing THP-1 cells, they grew too slow...Need help... - (reply: 11)
     
339. Poor ligation efficiency using Invitrogen TOP10 and XLI-blue cells - (reply: 5)
     
340. cell migration - (reply: 1)
     
341. Primary gastric epithelial cells - (reply: 1)
     
342. Crystal violet to determine cell numbers - (reply: 1)
     
343. Ca++ estimation in the cell - (reply: 1)
     
344. Do RAW cells (high passage number) lyse in PBS? - (reply: 2)
     
345. picking a cell line to perform cell transformation assays - (reply: 2)
     
346. red blood cells and the HA Assay - differences between species (reply: 6)
     
347. cell differentiation - (reply: 2)
     
348. How much media needed for cells in different cell culture plates - (reply: 3)
     
349. Culture of Epithelial cells from Breast Milk? - (reply: 2)
     
350. Cell separation - Cell separation (reply: 1)
     
351. HEP G2 cells - growth medium - (reply: 2)
     
352. Very high confluence after thawing cells– good or bad? - (reply: 8)
     
353. poor cell growth following FBS batch change - (reply: 5)
     
354. Cell Proliferation inhibition - arrest? (reply: 4)
     
355. CHO cells medium - (reply: 5)
     
356. Jurkat Cell Adhesion - Techniques to make Jurkat Cells to adhere to plastic surfaces (reply: 2)
     
357. Paradoxical cell count - Increase in cell count wihtout (known) stimuli (reply: 2)
     
358. Cell clumping in culture: Folic acid or i-Inositol? - (reply: 3)
     
359. Can the current technology make a human cell solely using materials from nature? - Can the current technology make a human cell solely using materials fr (reply: 2)
     
360. count cell number - (reply: 3)
     
361. Could we grwo chinese hamster cells in untreated normal petri dishes? - (reply: 1)
     
362. Could the strange G418 resistance fo my Chinese hamser cell lines be due to the - CAN I USE UNTREAED plates for selection? (reply: 2)
     
363. Is stem cell ethical? - Do you support or oppose it? (reply: 2)
     
364. stable cell screen - (reply: 2)
     
365. ES cell targeting - (reply: 2)
     
366. Are adherent THP-1 cells macrophages? - (reply: 1)
     
367. vehicle treated cells? - (reply: 2)
     
368. Microscope Settings - Microscope Settings for Trypan Blue Exclusion Assay (reply: 1)
     
369. Home made DH5alpha chemically comp. cells - (reply: 5)
     
370. siRNA transfection in endothelial cells - (reply: 3)
     
371. Growing E.coli cells - (reply: 1)
     
372. Can digest HESCs (H1) with trypsin/EDTA? - (reply: 1)
     
373. How to confirm whether the cell line was successfully transfected with two plasm - Co-transfection of two plasmids to 293 cells (reply: 6)
     
374. IPTG induction - at what cell density (reply: 5)
     
375. how to test the efficiency of chemically competent cells - (reply: 2)
     
376. whole cell sample vs cell lysate - (reply: 2)
     
377. gene down-regulated in cancer cell line but no proliferation inhibit ability - (reply: 3)
     
378. siRNA plus drugs - weird cell morphology (reply: 3)
     
379. sudden cell death- Please HELP - (reply: 6)
     
380. Is it Okay to provide 5%CO2 to insect cells SF21? - (reply: 4)
     
381. cell adhesion problems in outer wells for transfeciton - (reply: 1)
     
382. identify cell lines - (reply: 4)
     
383. cell lysis buffer - (reply: 4)
     
384. Does Trypan Blue expire? - (reply: 3)
     
385. Tyrosinase extraction from melanoma cells - (reply: 7)
     
386. RNA isolation from small amounts of cells - (reply: 1)
     
387. bacterial cell counter - Bacterial cell counter, need to count bacterial cell by automatic inst (reply: 2)
     
388. DNA extraction from PFA fixed cells - (reply: 2)
     
389. analysis of yeast cell cycle - problem (reply: 2)
     
390. Help of establishing stable cell line - (reply: 1)
     
391. How to detach adherent cells for Annexin V staining in FACScan - (reply: 1)
     
392. Cell lysis method for running cell lysate on Western blot - (reply: 4)
     
393. culture of spleen cell - (reply: 2)
     
394. Problems with making efficient chemically competent cells - (reply: 2)
     
395. prepare the SCLC for the transfection - SCLC cell seeding for transfection (reply: 4)
     
396. N in Cell Culture - What is the proper way to determine your "n"? (reply: 2)
     
397. PFA-Fixation of cells... why do they look crappy? - PFA-fixation of epithelial cells (reply: 2)
     
398. Can cell debris move? - (reply: 4)
     
399. PBS with or without Calcium and Magnesium - When it is important to use either one in Cell culture?? (reply: 2)
     
400. MLN cell isolation....Help! - (reply: 1)
     
401. information for cell lines - OTHER than ATCC (reply: 4)
     
402. Pansorbin cells - (reply: 3)
     
403. Collenchyma or sclerenchyma cells in monocot /dicot leaves - (reply: 1)
     
404. double transfection plus siRNA - in HEK293 cells (reply: 1)
     
405. how to extract plasmids leaving bacteria cells intact - (reply: 7)
     
406. cell line culture - (reply: 2)
     
407. Clumping and contamination in cell culture - (reply: 2)
     
408. Chromosomal aberration assay for ES cells - (reply: 1)
     
409. PBS buffer for cell isolation sing dynabeads - (reply: 2)
     
410. Unexplained Cell Culture Death - Expertise needed (reply: 7)
     
411. Nuclear Extraction kit for Primary Cell? - What's a good one? (reply: 1)
     
412. transfecting siRNA into primary fetal mouse hepatocytes - (reply: 6)
     
413. Protocols for Preparation of cell lysate from tissue sample - (reply: 3)
     
414. New to Cell Culture and need some basic information - help needed (reply: 12)
     
415. Cell passage question - (reply: 4)
     
416. HEK 293T cells - problems getting proper attachment. - (reply: 9)
     
417. Calcium Flux by FACS using Fluo-4 - Can the cells be fixed? (reply: 1)
     
418. Transfection of 293-T cells in 6-well plate, using PEI - (reply: 4)
     
419. Cell lysate at RT - (reply: 4)
     
420. plentivirus - stable cell line (reply: 1)
     
421. Monocyte derived Dendritic cells - (reply: 4)
     
422. Cell Culture Question - (reply: 3)
     
423. what is the media for HUVEC cells? - (reply: 4)
     
424. General procedure for passing (splitting) cells - (reply: 3)
     
425. Confluent 25 cm2 flask means how many cells? - quick question, need quick answer (reply: 1)
     
426. What's a good Nuclear extraction kit for primary cell? - (reply: 1)
     
427. THP-1 serum free cells - (reply: 3)
     
428. Establishing thymoctyes cell lines - (reply: 1)
     
429. Can enzyme kinetics be measured in whole cell extracts? - (reply: 3)
     
430. yeast specialists - strange behavior in yeast cells (reply: 9)
     
431. cell scraping - I DONT recommend it for subculturing (reply: 15)
     
432. media or cell problem? - (reply: 5)
     
433. what kind of contamination is this? (photos inside) - contamination in cell culture with HeLa (reply: 10)
     
434. quenching of GFP after selection of stable cells - (reply: 1)
     
435. cell lysate PAGE & blotting problem? - (reply: 1)
     
436. stable cell line, but quenched GFP? - (reply: 5)
     
437. Question about nontreated cell cultureware - (reply: 5)
     
438. PCR on Genomic DNA from cell lysate - (reply: 5)
     
439. looking for HCV positive cell lines? - (reply: 6)
     
440. dermal multipotent cells - (reply: 1)
     
441. what would happen if i dilute protease inhibitor too much in cell lysate? - (reply: 3)
     
442. cell line contamination - what to do? (reply: 7)
     
443. A problem about infecting primary T cells - (reply: 1)
     
444. contamination in MACS seperated cells - (reply: 3)
     
445. what to do with the differentiated mouse es cells - (reply: 3)
     
446. multiple plasmids in cells - (reply: 1)
     
447. What is the pI of most of proteins in cell? - (reply: 3)
     
448. Fungal contamination in cell lines! - (reply: 2)
     
449. easy & quick apoptosis assay for siRNA treated cell line sample - (reply: 3)
     
450. Can anyone tell me why my cells are dying? - My mouse embryonic stem cells are dying and I'm baffled as to why. (reply: 2)
     
451. plasmid transfection in hepatocytes - (reply: 1)
     
452. siRNA transfection in primary hepatocytes - (reply: 1)
     
453. primary hepatocytes as hepatocarcinogenesis model - (reply: 3)
     
454. Loading cell pellet in SDS-PAGE - (reply: 4)
     
455. Measuring cells in micrographs - (reply: 1)
     
456. What happen to my NIH3T3 cells? - (reply: 6)
     
457. CELL LYSIS - reasons behind protocols (reply: 2)
     
458. perovskite oxide nanowires in living cells - (reply: 1)
     
459. Getting Cells to adhere STRONGLY - help for high throughput screen (reply: 9)
     
460. Really, isn't there anyone who can recommend a TUNEL assay? - for adherent cells using fluorescent microscopy? (reply: 2)
     
461. competent Bascillus subtilis cells - (reply: 2)
     
462. Fixing cells in wells? - (reply: 4)
     
463. Transfection of siRNA in suspension cells - (reply: 6)
     
464. Problem with thawing cells from liquid nitrogen? - (reply: 6)
     
465. Observed tiny black dots on cell culture - (reply: 9)
     
466. Problem with mouse embryonic stem cells - contamination? - (reply: 1)
     
467. RNA extraction from plant cell cultures - (reply: 2)
     
468. Cell immortalize - (reply: 2)
     
469. Why cells should be washed before electroporation? - (reply: 2)
     
470. TUNEL assay- should I grow cells on slides? - Or label cells in suspension then deposit on slides? (reply: 1)
     
471. Lysis buffer used to get cell lysates need to be sterilised? - (reply: 8)
     
472. confirming how the gene causes decreased growth/increased cell death - (reply: 4)
     
473. storing fixed cells - (reply: 4)
     
474. To keep dead cells when harvesting? - (reply: 2)
     
475. Best method of storing cultured cells for later RNA isolation - (reply: 1)
     
476. anyone culture SKOV3 cells? what's the morphology under microscope? - (reply: 3)
     
477. how cells adhere to the surface of tissue culture flaask? - basic question (reply: 3)
     
478. how to sdudy the matter exchanges among cells? - (reply: 4)
     
479. Effect of trypsin on cells in FACS - (reply: 3)
     
480. RAW 264.7 cell transfection - Probs transfecting RAW cells with lipofectamine LTX (reply: 2)
     
481. How do Phoenix cells produce virus - (reply: 1)
     
482. Transfecting P19 embryonic carcinoma cells - (reply: 2)
     
483. Restaining cells after cryopreservation - (reply: 7)
     
484. Transfecting NS0 cells - (reply: 5)
     
485. Freezing cells before lysis? - (reply: 4)
     
486. Calculating cell purity after MACS enrichment - (reply: 1)
     
487. Help with transfection of HEK cells with p53 flag - (reply: 5)
     
488. Role of Trypsin in Hep G2 culture - (reply: 1)
     
489. In vivo T cell depletion - (reply: 1)
     
490. Chemoresistance assay - How to measure the chemoresistance of cancer cells (reply: 6)
     
491. HEK cell, EGFP and PI staning - (reply: 7)
     
492. is a matrigel layer a 3d cell culture? - (reply: 6)
     
493. ligation transformation.......which chemically competent cells are the best? - (reply: 3)
     
494. how to cryopreserve HepG2 cells at -80 degrees? - (reply: 6)
     
495. irradiated 3T3 cells as feeder cells - (reply: 2)
     
496. hemin in cell cultures - (reply: 8)
     
497. Is it true that a cell line cannot be infected with virus again once it was alre - virus infection (reply: 1)
     
498. Cells not sticking to chamber slides after fixing - (reply: 8)
     
499. Cell lysis buffer for real time RT-PCR - Request for cell lysis buffer recipe or relevant scientific paper (reply: 1)
     
500. Amount of Cell Lysate for Western Blot - (reply: 2)
     
501. Where can we purchase human microvascular endothelial cells? - (reply: 1)
     
502. stable transfected cell line in absence of G418 - (reply: 6)
     
503. stimulation of cell division/mitosis? - of non-dividing exocrine cells (reply: 4)
     
504. N2a cells differentiation - (reply: 4)
     
505. How to make Matrigel layer as thin as humanly possible? - Our technique relies on a VERY close contact of cell with coverslip (reply: 5)
     
506. Tris and cell toxicity - (reply: 4)
     
507. Maintaining stable cell line - (reply: 5)
     
508. cell differentiation and survival - (reply: 4)
     
509. Stable cell line - clone vs. population - (reply: 15)
     
510. DNA laddering in Smooth Muscle Cells - (reply: 1)
     
511. How to isolate gastric parietal cells? - Do you have protocol for isolation gastric parietal cell? (reply: 3)
     
512. Can I store bacteria cells at +4C before plasmid isolation? - (reply: 3)
     
513. how to distinguish normal and cancerous osteoblast cells - (reply: 3)
     
514. EGFP stable cell line proof - (reply: 3)
     
515. How to kill proliferating cells but not arresting cells? - (reply: 3)
     
516. Does sonication of cell lysate.... - (reply: 2)
     
517. Cell contamination - Small rapidly moving black rods (reply: 18)
     
518. Stem Cell Culture Medium - Stem Cell Culture (reply: 1)
     
519. TMK 1 Cell - Where can I buy TMK 1 cells? (reply: 1)
     
520. rat bone marrow mast cell maturation - Anyone have any experience or advice? (reply: 1)
     
521. Agent to punch hole in cell membranes - (reply: 1)
     
522. Cell Viability Dyes Better than Trypan Blue - Trypan blue vs Erythrosine B vs Other? (reply: 4)
     
523. Yeast Competent Cells - (reply: 34)
     
524. Does formaldehyde crosslink completely kill cell? - (reply: 2)
     
525. cell lysate for immunoprecipitation - (reply: 2)
     
526. HeLa cells - safety concern - (reply: 2)
     
527. Transient Transfection of HeLa cells. - (reply: 1)
     
528. bc cell differentiation - T47ds (reply: 1)
     
529. Cell Storage Conditions for Cell Cycle Analysis - PI staining with GFP containing construct (reply: 2)
     
530. Where to buy gastric parietal cell lines? - (reply: 1)
     
531. reviving corneal cell line - (reply: 1)
     
532. Morphology of ES cell clones - Does this clone look like ES cells? (reply: 4)
     
533. Calculating cells/well in a 96-well plate - Calculating cells/well in a 96-well plate (reply: 8)
     
534. Can any one help to transfect these cells ? - SCN neuron transfection (reply: 2)
     
535. ChIP cell lysis buffer - what lysis buffer do you use for Chromatin IPs (reply: 6)
     
536. cell counting again - (reply: 2)
     
537. Human dendritic cell line - (reply: 5)
     
538. How to send cells for immunfluorescent staining? - (reply: 8)
     
539. About trypsin without phenol red - (reply: 3)
     
540. Cells dying upon temperature shift. - (reply: 7)
     
541. Separation of inclusion bodies from cell debris - (reply: 2)
     
542. Spermatogonia stem cell culture - (reply: 4)
     
543. Rapid growth of transformed DH5 alpha cells - (reply: 4)
     
544. THP-1 cell culture problem: Cells die after thawing - (reply: 4)
     
545. number of Cos7 cells on a 10cm plate - (reply: 2)
     
546. Fixation of cells for long time storage - (reply: 1)
     
547. HL 60 cell - (reply: 1)
     
548. cell migration, cell invasion and cell proliferation assay - (reply: 5)
     
549. Concentration of miRNA in cells? - (reply: 1)
     
550. cell culture - problem with suspension CHO cell revival (reply: 2)
     
551. Some basic questions about cell fractionation and incl.bodies - (reply: 2)
     
552. Optimization of seeding cells number for MTT assay - (reply: 5)
     
553. Cell culture using laminin - (reply: 1)
     
554. MCF-7 cell grow too slow - (reply: 5)
     
555. Living cell GFP imaging - (reply: 1)
     
556. Living cell GFP imaging - (reply: 6)
     
557. lost contact inhibition=oncogenic cells? - (reply: 3)
     
558. weird S2 cells - (reply: 1)
     
559. to many dead cells for FACS - how to prepare "sheroid" cells gently for FACS (reply: 2)
     
560. neuronal cell suspension from brain - (reply: 4)
     
561. Cell isolation from tissue -- how do you tell how many viable cells you get? - (reply: 3)
     
562. Transfection of Drosophila cell culture - Which is the best transfecting agent? (reply: 1)
     
563. Interested in growth characterstics of HER2 expressing cell lines; SKOV-3,NCI-N8 - (reply: 1)
     
564. A CO2 independent medium limit the range of cell types that can be investigated? - (reply: 1)
     
565. cell fractionation and obtaining pure nuclei - (reply: 1)
     
566. Centrifugation speed for adherent cell line - (reply: 11)
     
567. Transfection of L929 cells with Poly (IC) - (reply: 1)
     
568. time required for cell recovery after thawing - (reply: 8)
     
569. Competent Cell Preparation - (reply: 2)
     
570. Competent Listeria Cells - (reply: 3)
     
571. Temperature for growth of skin cells - (reply: 3)
     
572. Transformation of pCMV-sport6 vector to DH5 alpha competent cells - (reply: 1)
     
573. how to know the plasmid or phage inside the cell? - (reply: 3)
     
574. Protein extraction from animal cells - (reply: 7)
     
575. why dendritic cell died in phagocytosis assay? - (reply: 5)
     
576. Sepration of cells from cell debris in sample - (reply: 2)
     
577. BHK Cell Transfection - (reply: 2)
     
578. Help: Small RNA (siRNA) isolation from cultured cells - (reply: 5)
     
579. protein expression in NSO cells - (reply: 6)
     
580. HBSS vs PBS to deliver compounds to cells - (reply: 3)
     
581. Transferrin-alexa fluor633 can not be endocytosed by Vero cells. Who knows why? - (reply: 4)
     
582. centrifugation speed for fixed cell - (reply: 2)
     
583. Is TCA really specific in precipitating proteins from cell lysate? - (reply: 1)
     
584. competent cell contamination? - (reply: 3)
     
585. How to lyse animal cell line? - (reply: 17)
     
586. Lysis of fat cells/adipocytes - (reply: 7)
     
587. Cell Lysis - (reply: 2)
     
588. CHO-S cell - (reply: 1)
     
589. protein isolation from cell lines - membrane bound proteins (reply: 3)
     
590. cell lines - (reply: 2)
     
591. why there are bubble in cell? - (reply: 3)
     
592. On trypsin neutralizing solution - (reply: 2)
     
593. More deep ChIPping woes (Sonication Optimization) - Primary cell line.... (reply: 1)
     
594. Drug treatment enhanced cell growth? - (reply: 1)
     
595. how many cells in a mouse spleen - (reply: 5)
     
596. problem with transformation - ligation and cells seems to be fine (reply: 3)
     
597. The BEST cell counting technique? - (reply: 4)
     
598. Wanted! Protocol Immunocytochemistry with HRP-DAB reaction - Need Non-fluorescent immunostaining on cultured cells (reply: 2)
     
599. cell harvesting; use of antibiotics? - (reply: 8)
     
600. dead cell staining followed by fixation? - (reply: 4)
     
601. Best Guess:How many cells in Organotypic Slice culture - (reply: 2)
     
602. can hyaluronidase be used to detach/digest cells? - (reply: 1)
     
603. What are the negative effects of HEPES on cells - if any - (reply: 1)
     
604. Cell Suspension for MRI Study - (reply: 3)
     
605. HELP: transfection reagent for BJ cells - (reply: 1)
     
606. Search more efficient transfection reagent for BJ cells - (reply: 1)
     
607. Search transfection reagent for BJ cells - (reply: 2)
     
608. Unexplained cell death - (reply: 6)
     
609. mycoplasma effects - looking for a reference (reply: 5)
     
610. Why cells are so resistant to G418? - G418 cannot kill cells (reply: 6)
     
611. Apoptosis by PI staining on epithelial cells? - (reply: 3)
     
612. what's wrong with my MTT assay on a suspension cell line - (reply: 8)
     
613. cell density for checking certain compound - (reply: 2)
     
614. Ooops! I messed up! Help! - freezing cells (reply: 5)
     
615. Dissotiation of cells from the bottom of a transwell - (reply: 2)
     
616. Co-IP in sonicated whole cell lysates - (reply: 3)
     
617. staining cells on transwell inserts - fluorescence microscopy (reply: 3)
     
618. no GFP in cells - (reply: 7)
     
619. Problem with cell harvest - cell harvest (reply: 6)
     
620. SLOW cell growth - (reply: 2)
     
621. Protein expression help needed - Mammalian cells (reply: 11)
     
622. Apyrase in cell culture - Half-life? (reply: 3)
     
623. DMSO in cell culture - is it toxic, does it change signalling? - Citations wellcommed (reply: 2)
     
624. Cells are lost after fixation - (reply: 14)
     
625. How long to wait until cell activation after trypsine use? - (reply: 6)
     
626. primary sertoli cell transfection - (reply: 2)
     
627. Force "swimming" cells to adhere - Are there any non-toxic detergents for cell culture? (reply: 4)
     
628. how to separate dead from viable cells in cultures of non-adherent cells - (reply: 3)
     
629. Identification of monomers/dimers in whole cell lysate via SDS-PAGE - (reply: 3)
     
630. Antibodies for immunohistochemistry and cell ELISA - Are they the same? (reply: 2)
     
631. suspension culture of lymphoblastic cells - (reply: 7)
     
632. Hela cell contamination? - (reply: 10)
     
633. THP-1 cells culture - (reply: 4)
     
634. Cat No of M199 medium for endothelial cells URGENT - (reply: 1)
     
635. HEK 293 cell culture cytotoxicity? - (reply: 2)
     
636. brdu staining on gfp+ve cells - (reply: 1)
     
637. How to Transfect T cells - (reply: 2)
     
638. How to infect T cells? Lentiviral or retroviral? - (reply: 2)
     
639. Doesn't anyone use roller bottles to culture cells in anymore? - Let's compare notes. (reply: 2)
     
640. dendritic cells culture - (reply: 3)
     
641. help!pearl necklace like structure in CHO cell? - pearl necklace like structure in CHO cell? (reply: 1)
     
642. cell lysis and save the ECM - (reply: 1)
     
643. Pipetting stress on cells - (reply: 5)
     
644. Harvesting cells following transfection - Scrapping or trypsin (reply: 2)
     
645. losing protein expression after stablizing cells( please help) - (reply: 4)
     
646. Competent cells not growing - (reply: 5)
     
647. Equal number of cells per well (96 well plate) - (reply: 1)
     
648. preparation of bacterial cell lysate - how to do? (reply: 1)
     
649. Smooth muscle cell culture - (reply: 4)
     
650. Looking for a good cell line to study beta-catenin activity using transient tran - Please recommend a cell line that is good for transient transfection o (reply: 3)
     
651. Contamination by packaging cells? - (reply: 5)
     
652. Preparation of Cell Lysate for Western Blot - What did I do wrong? (reply: 14)
     
653. best con. of trypsin with neuro cells? - (reply: 9)
     
654. Antibodies to stain vesicles/vacuoles in my cells? - (reply: 4)
     
655. How to select a cell type to study my gene - (reply: 2)
     
656. Cell fractioning in order to perform binding assay - (reply: 1)
     
657. cos-7 cells transfection using calcium phosphate - (reply: 1)
     
658. competent cell didn't grow in LB - (reply: 15)
     
659. Problems in CaCl2 method ofr competent cells - (reply: 1)
     
660. BrDU staining on GFP positive cells - (reply: 1)
     
661. Electro-competent cells. How do I make them "more" competent? - Need advice on increasing the efficiency of Top 10 E. coli (reply: 10)
     
662. Contamination or not? - Cell Culture Contamination (reply: 1)
     
663. cell attachment assay - which one and how? - (reply: 6)
     
664. protocol for Trypan Blue exclusion assay - How to do trypan blue exclusiona ssay on adherant cells? (reply: 3)
     
665. Bradford vs 280 protein quantification - BSA standard vs cell lysates (reply: 4)
     
666. Infection of human cell lines grown in suspension? - Infection protocols (reply: 3)
     
667. cytokines anf growth factor expression in bone marrow mesenchymal stem cells - Stem cells (reply: 3)
     
668. Need advice for growing THP-1 cells - (reply: 4)
     
669. suspencion cell - electroporation with BTX830 apparatus - TROUBLE WİTH SUSPENSİON CELLS (reply: 6)
     
670. Live cell nuclei immunostaining - (reply: 2)
     
671. Contamination of cultured cells - (reply: 23)
     
672. Does a B lymphocyte contain lysosome inside? - Cell Structure (reply: 1)
     
673. stage at which transfection should be done, when cells are round or flat adhered - (reply: 4)
     
674. Cell fractionation-help! - (reply: 6)
     
675. Western blot with Adipose cells - (reply: 1)
     
676. Nystatin makes vero cells round, too high concentration? - (reply: 3)
     
677. if Annexin-v and PI staining is suitable to attached cells - (reply: 3)
     
678. How to separate cells in urine for FISH? - (reply: 1)
     
679. Dopaminergic cell lines - Need info on DAergic cell lines (reply: 2)
     
680. CaCl2 Competent Cell - (reply: 3)
     
681. western blots - survivin - detection of survivin in Panc1 cells (reply: 2)
     
682. Does trypsin digest integral plasma membrane proteins? - (reply: 2)
     
683. culturing trouble making competent cells - (reply: 1)
     
684. passage of cell culture and protein locolization - (reply: 1)
     
685. How to pick cell from soft agar and recover it? - (reply: 2)
     
686. ChIP assay using Matrigel-3D cell culture - (reply: 2)
     
687. Schock freezing - cell disruption? - (reply: 3)
     
688. quantifying my gene expression in 5 different cell lines - (reply: 1)
     
689. Culturing of MUTZ-3 cells - (reply: 1)
     
690. Counting cells using ELISA method? - Counting cells using ELISA method (reply: 1)
     
691. Adipocytes from stem cells? - (reply: 6)
     
692. trypan blue - (reply: 2)
     
693. PCR variability or bad cell source? - (reply: 1)
     
694. culture CD34+ Hematopoietic Stem Cells - (reply: 2)
     
695. help with site-directed mutagenesis - What polymerase and cells to use? (reply: 3)
     
696. Preparing cell lysate from cell culture for Western blot - (reply: 4)
     
697. Plasmid replication in mammalian cell systems - (reply: 3)
     
698. How to introduce DNA to animal cells? - (reply: 3)
     
699. Problem with growing up from cell stocks - (reply: 3)
     
700. MTT assay + suspension cells - (reply: 4)
     
701. gene targeting in ES cells - (reply: 1)
     
702. Western Blot of cells in Laemmil - (reply: 5)
     
703. neuro stem cells vs. neuroblastoma cells - (reply: 1)
     
704. AML cell lines - Where can I get them?? (reply: 1)
     
705. Clumping of normal colon cells due to transfection reagent - Counting problems (reply: 6)
     
706. prepare electrocompetent cells - (reply: 4)
     
707. why my suspension cells changed to adherent cells? - (reply: 3)
     
708. CO2 monitor for incubator - What do you guys do? - Suspicious cell stress/death (reply: 4)
     
709. 3H Thymidine assay with non-adherent cells / cell harvester - cell harvester a must? alternatives? (reply: 2)
     
710. plating caco-2 cells - Caco-2 cells don't form monolayer and MEM turned pink!! why?? (reply: 2)
     
711. human serum in cell culture - is it possible to use human serum in cell culture? (reply: 14)
     
712. Recommendations - - cheap, quick and cell safe Rnase a inhibitor/inactivator - (reply: 1)
     
713. Trypan blue & methylene blue - both to measure cell viability? (reply: 2)
     
714. Just how many cells are lost when changing tubes? - (reply: 5)
     
715. collect secreted proteins in cell culture media - (reply: 2)
     
716. Cell cycle: ModFIT vs. FlowJo - diferent percentages outcomes on same histograms (reply: 1)
     
717. Where exactly are mesenchymal stem cells located in bone marrow? - (reply: 1)
     
718. problem with plating adherent cells in 24-well plates - (reply: 11)
     
719. Jurkat/J77 cell line specifics - (reply: 2)
     
720. Raji Cell Histone Immunocytochemistry and Confocal Help - (reply: 1)
     
721. DNA isolation from small amount of cells - (reply: 1)
     
722. Cell Seeding and Drug treatment - (reply: 12)
     
723. unable to trasnfect Hela cells - (reply: 6)
     
724. Embryonic stem cell culture - (reply: 4)
     
725. DOES DMSO CHANGE THE CELL? - (reply: 2)
     
726. cell biology video clips - (reply: 2)
     
727. androgen solution for cell treatment - (reply: 7)
     
728. cell seeding confusion - what does it mean (reply: 1)
     
729. Basic help in cell counting...... - (reply: 6)
     
730. Using Adenovirus to transfect cells - (reply: 5)
     
731. PPARs expression in cardiac cells - (reply: 4)
     
732. Cell assay for ROS measurment - (reply: 1)
     
733. How do I culture adherent TZM-bl cells?! - (reply: 5)
     
734. Red Cells in Clear Ficoll Layer Following Density Centrifugation - Monocyte Isolation (reply: 2)
     
735. Breaking yeast cells - (reply: 2)
     
736. preparation of electroporation competent cells from chemically competent cells - (reply: 3)
     
737. cotransfection of CHO cells - (reply: 1)
     
738. cell culture - passage number (reply: 7)
     
739. can plasma (instead of serum) be used on cell culture? - (reply: 2)
     
740. do the competen cells chang the U to T - (reply: 4)
     
741. RAW cell line transfection - (reply: 2)
     
742. increased BrDU incorporation but similiar cell cycle profile by PI or DAPI - (reply: 2)
     
743. When should I suspect there is a Mycoplasma contamination? - (reply: 10)
     
744. help! cell culture contamination too! - (reply: 5)
     
745. E.coli TOP10 comp cells T1 Phage resistant? - (reply: 1)
     
746. recommendations for a viral titer cell line - (reply: 3)
     
747. what time is better to seed cells in well or dishes before transfection? - (reply: 7)
     
748. Subculturing primary human fibroblasts - Seeding density? (reply: 1)
     
749. reusable cell culture flasks, pipettes etc. - (reply: 10)
     
750. Experiment with KATO III cells - (reply: 1)
     
751. please give me some suggestions on preparating Yeast cells with PI stainning fo - buffer ,PI solution (reply: 4)
     
752. SiRNA in MIN6 cells, nucleofection - (reply: 1)
     
753. Single cell luciferase bioluminescence detection. Is it possible? - (reply: 1)
     
754. Cell passage number for non-cancerous cells - (reply: 2)
     
755. Sodium pyruvate in ES cell culture media - (reply: 2)
     
756. Cell death with DMSO treatment, during transfection using polybrene - (reply: 2)
     
757. Fixing non adherent cells - (reply: 3)
     
758. stable transfection high expression - problems with low expression in stably transfected cells (reply: 1)
     
759. A good list of literatures for primary cell isolation - (reply: 3)
     
760. Homework question - what happens to cells when they're put in distilled wate - (reply: 3)
     
761. How to digest my cell line/strain to a single-cell suspension? - Help! (reply: 4)
     
762. looking for protocol using DAPI for cell cycle analysis - (reply: 1)
     
763. Whats the cell size - (reply: 1)
     
764. Synchronization of NIH 3T3 cells - (reply: 2)
     
765. B95-8 EBV producing cell line - need EBV producing cell line. (reply: 2)
     
766. difference between staining vital cell and dead - (reply: 9)
     
767. why should the conversion efficiency of competent cell be at least 10^8 cfu/_ - (reply: 2)
     
768. Question making competent bacterial cells - (reply: 2)
     
769. Enlarging Cells - (reply: 3)
     
770. Cell Growth Following Confluency - (reply: 2)
     
771. Stable cell line losing expression faster than usual - (reply: 6)
     
772. Transfecting primary cells in suspension - (reply: 4)
     
773. PCR from cell culture - to detect the presence of adenovirus (reply: 2)
     
774. primary colonic epithelial cell culture - (reply: 1)
     
775. Passaging stable transfected cells after thawing - (reply: 4)
     
776. trypsin concentrations for detaching cells - (reply: 2)
     
777. Cell culture - (reply: 2)
     
778. storing unstained cells overnight for FACS next day - (reply: 3)
     
779. Is that possible to establish a stable cell line which is transfected by an empt - (reply: 2)
     
780. Cell adheison assy on round surface - (reply: 1)
     
781. Could competent cell be stored in liquid nitrogen? - (reply: 7)
     
782. Effectively washing adherent cells? - how do I optimize washing? (reply: 3)
     
783. what happens if cells turn liquid? - (reply: 4)
     
784. Formaldehyde cross link after washing cells - (reply: 2)
     
785. Cell Fixation method - % of PFA ? (reply: 4)
     
786. cell synchronization - how to make cell stay at G0 phase (reply: 1)
     
787. culture cell - (reply: 1)
     
788. lysing cells on matrigel - (reply: 1)
     
789. Storing Induced E.Coli Cells - (reply: 1)
     
790. Formaldehyde Fix cells to prevent detergent lysis ? - Testing a protocol to make cells resistant to cell lysis buffer (reply: 2)
     
791. choosing competent cells - (reply: 5)
     
792. extractin RNA from cells grown on Matrigel - (reply: 1)
     
793. FACS sorting and culturing cells afterwards - (reply: 1)
     
794. siRNA stable cell lines - (reply: 6)
     
795. Secrets for declumping cell pellets - (reply: 5)
     
796. Best compotent cells - (reply: 5)
     
797. Mycoplasma - (reply: 2)
     
798. cell density for infection with baculovirus - what is the optimal cell dentisty at the point of infection (reply: 1)
     
799. mouse U6 promotor in human ES cells - (reply: 1)
     
800. Creating Cell suspension - (reply: 1)
     
801. Cell Surface Biotinylation - Advantage/Disadvantage (reply: 7)
     
802. pyridine, chloroform and metahnol as a solvent for chemopreventive agents - Is there anyone who has used these solvent for cell culture experiment (reply: 6)
     
803. changed attchment of RMA/S cells after transfection, why? - (reply: 2)
     
804. cell culture contamination - after transfection (reply: 15)
     
805. T antigen status of human cells for transfection - (reply: 1)
     
806. How to prepare my own competent cells? - as above (reply: 5)
     
807. removing rbc cells for cell purification - (reply: 5)
     
808. minimum amount of cells used for western blot - (reply: 5)
     
809. cell lysis and DNA - sticky (reply: 3)
     
810. Is it possible to transform competent cells with linear DNA? - (reply: 5)
     
811. 293T cells - (reply: 3)
     
812. natural killer cells - (reply: 2)
     
813. Frustrated with FACS (ONLY DEAD CELLS) - (reply: 8)
     
814. Problems obtaining stable transfected 293 cells - (reply: 1)
     
815. few cells in the cell culture - (reply: 3)
     
816. Help with different cell fixatives - Neuronal Fixation (reply: 7)
     
817. counting viable cells directly from wells - (reply: 7)
     
818. Stable transfection of H295R cells - (reply: 3)
     
819. how do i do flow cytometry for adherent cells? - (reply: 5)
     
820. tranfection of 3T3-L1 cells post differentiation - (reply: 2)
     
821. Problem with Monoclonal antibody production - why cells die after adding HAT?????? (reply: 6)
     
822. stainig of cells for FACS - (reply: 2)
     
823. Trypsin/EDTA - how it works? (reply: 1)
     
824. pBABE-puro in 293T cells (not PhoenixA) - (reply: 5)
     
825. Basic CHO cell culture - (reply: 3)
     
826. stable transfections using SCC 9 cells - (reply: 5)
     
827. Which vector would you choose for transient expression in CHO cells - (reply: 2)
     
828. Stable transfection of human adrenocortical cancer H295R cell line - (reply: 1)
     
829. constrcut a GFP plasmid that work in Drosophila s2 cells - (reply: 1)
     
830. any tips for detecting phospho-ptotein in fixed adherent cells by flow cytometry - (reply: 2)
     
831. Alternative Sera for Cell Culture - (reply: 6)
     
832. 293T cells: how to sort it the problem of detaching after washing with PBS - (reply: 3)
     
833. E.coli Compotent Cell Storage and Plasmid Isolation - (reply: 3)
     
834. How to seed adherent cells evenly in 150mm dishes? - (reply: 12)
     
835. Cell culture sterility Checks (DMEM) - (reply: 3)
     
836. PMA induced apoptosis in HEK cells - (reply: 4)
     
837. Use of urea for cell lysis - (reply: 1)
     
838. Why different mammalian cell lysate preparation methods? - (reply: 6)
     
839. how to maintain cell in serum free medium - (reply: 6)
     
840. cell lysis to investigate phosphorylated protein - (reply: 1)
     
841. growing cells on coverslip - (reply: 6)
     
842. centrifugation speed for mouse neural stem cells - centrifugation speed for mouse neural stem cells (reply: 3)
     
843. GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
     
844. cy5 cell tracker dye - (reply: 2)
     
845. Which cell line for Cell cycle analysis - (reply: 1)
     
846. transformation competent cells problem - fastbac vectors (reply: 2)
     
847. RT-QPCR of cells treated with 5-aza - which housekeeping gene to use, different cell lines (reply: 1)
     
848. Mortality with Lipofectamin RNAiMax and RPE-1 cells - (reply: 8)
     
849. cell starvation - which medium is good? (reply: 4)
     
850. transformation of competent cells - (reply: 2)
     
851. Electroporation with a pool of vectors with different inserts - why only one kind of vector maintained in the cells? (reply: 2)
     
852. Test for drug dose response in suspension cells before transfection - With Suspension cells, how do people distinguish difference between de (reply: 1)
     
853. freezing cells in log phase - (reply: 2)
     
854. adipose stains? - specific stain to positively ID adipose cells (reply: 2)
     
855. chromatin yield (ChIP) - chromatin yield from primary cells (reply: 3)
     
856. Are there antibodies to differentiate epithelial from fibroblasts cells? - (reply: 5)
     
857. Cell washing - Can I use Tween/Triton etc? (reply: 7)
     
858. isolation of total RNA from bacteria attached to HeLa cells - (reply: 1)
     
859. Poly-lysine treatment and cell removing - (reply: 4)
     
860. SURE E. coli strain - How make competents cells? (reply: 4)
     
861. Monocytic cell line - (reply: 1)
     
862. APC isolation from the Liver - How you can isolate the APC cells from the mice's liver (reply: 2)
     
863. thawed cells dying - (reply: 6)
     
864. Cell culture pronunciation - pas-SAGE or PAS-sage? (reply: 7)
     
865. stable transfection for suspension cell lines - (reply: 1)
     
866. differentiated PC12 cells detatching - (reply: 3)
     
867. automatic cell counting for Microscope images - (reply: 2)
     
868. Thp-1 cell transduction - (reply: 4)
     
869. loading control for nuclear protein in rabbit cell extract - (reply: 1)
     
870. HIV-2 and MTT assay, cells lines to use - (reply: 2)
     
871. problems about THP-1 cell culture - (reply: 1)
     
872. tissue preparation for human/mouse islet cells - (reply: 1)
     
873. Autoclaving cell media - Necessary to autoclave sub-solutions? (reply: 4)
     
874. Protein Extraction from cell media for western blot - (reply: 3)
     
875. adherent cell don't need detach for maintenance? - (reply: 2)
     
876. cell adhesion to plastic - (reply: 3)
     
877. How to reduce cell autofluorescence? - (reply: 5)
     
878. Can I use a cell free extract on Mass Spec? - (reply: 8)
     
879. culture contamination...yeast, bacteria, mycoplasma - (reply: 3)
     
880. Chemically competent cells? - (reply: 3)
     
881. seeding hela cells from ATCC - (reply: 4)
     
882. cell culture with serum media and with serum free media and doubts in luciferase - (reply: 1)
     
883. Immunocytochemistry in neural stem cells - (reply: 1)
     
884. bath sonicator for cell lysis - (reply: 2)
     
885. BHK cells transfection - (reply: 2)
     
886. serum starvation - induce cilia by serum starvation in Rpe-1 cells (reply: 7)
     
887. Cell seeding - (reply: 3)
     
888. Keep growing cells after an MTS/PMS assay - (reply: 1)
     
889. Cells in log phase - (reply: 1)
     
890. RNA in HeLa cell - amount of RNA in a HeLa cell (reply: 2)
     
891. protocol for transfection(eletroporation) for HEK293 cell - (reply: 1)
     
892. Cell fractionation: nuclei do not pellet - (reply: 2)
     
893. Horrible mycoplasma contamination - (reply: 5)
     
894. Stable cell line, polyclonal and monoclonal. - Which is better? (reply: 4)
     
895. Red Blood Cell percentage in human spleen - (reply: 4)
     
896. lentiviral transduction of suspension cell - (reply: 1)
     
897. Can cell culture media be frozen at -20°C? - (reply: 6)
     
898. cell counting dilemma - (reply: 1)
     
899. Passaging of cells - need to remove in-activated trypsin? - (reply: 8)
     
900. lentiviral transduction of suspension cell - (reply: 1)
     
901. Neuroblastoma cell line - which are commonly used as standard cell lines? (reply: 3)
     
902. Immunostaining on bacterial cells? - (reply: 1)
     
903. Can cell culture media be frozen at -20°C - (reply: 4)
     
904. any kit or reagent for extracting both DNA and RNA from the same cell culture - (reply: 3)
     
905. how to prepare Abeta for cell culture - how to the solution sterile (reply: 1)
     
906. FACS sorting affects cell function? - FACS sorting affects cell function (reply: 2)
     
907. medium -preparation for CD34 cells - (reply: 2)
     
908. cell counting calculation.... - (reply: 3)
     
909. Transfection of cryopreservative cells - (reply: 3)
     
910. Phoenix 293T packaging cells - (reply: 3)
     
911. Temperature for fixing cells in immunofluorescence microscopy - (reply: 6)
     
912. suparnatant, cell free extract, crude extract - (reply: 4)
     
913. muscle vs. fat cells - (reply: 3)
     
914. competent cells transform 12 kb plasmid,urgent - (reply: 8)
     
915. Fixing the Endothelial Cells - (reply: 7)
     
916. Whole cell extract on SDS/PAGE - (reply: 9)
     
917. Bacterial Cell Density - (reply: 5)
     
918. Making competent cells from current commercial stocks - (reply: 3)
     
919. what affects cell culture viabilty? - (reply: 8)
     
920. Calculation cell in 96 well plate - (reply: 1)
     
921. Tween 20 and Cells - Question - (reply: 1)
     
922. G418 and resistant cells - can't kill the cells (reply: 8)
     
923. Drosphila S2 cell culture - (reply: 1)
     
924. MIN6 cells - (reply: 9)
     
925. What cell lines are good for lymphoma research? - Please recommend lymphoma cell line. (reply: 4)
     
926. cell signaling & choice of the cell - (reply: 3)
     
927. PT67 cell contamination after transfection - antibody against PT67 (reply: 1)
     
928. hard to transfect cell lines - (reply: 1)
     
929. can cell scraper be reused - (reply: 7)
     
930. How can I separate nuclei from cytoplasm in trophoblastic cells - (reply: 1)
     
931. Modification of Hematopoietic stem cell - (reply: 7)
     
932. salt conc in lysis/homogenization buffer? - effect of different salt (NaCl) conc on cell lysis? (reply: 3)
     
933. cell suspension of CD34 cell isolated from whole blood - (reply: 3)
     
934. Primary culture - Transparent cell? (reply: 2)
     
935. Whole cells versus supernatants? - (reply: 1)
     
936. transfection of chick cell - (reply: 3)
     
937. 721.221 lymphoblastoid cell line - (reply: 1)
     
938. non-adhernt cell washing - (reply: 1)
     
939. MIF cells? - (reply: 1)
     
940. Immunology on fixed bacteria - What are the basics of fixing cells for immunological analysis? (reply: 1)
     
941. Purifying His-Tagged Proteins from Insects cells on IMAC resin? - (reply: 1)
     
942. transfection of PC12 cell line - (reply: 1)
     
943. Best lysis buffer for mammalian cells to do affinity chromatography? - (reply: 3)
     
944. Assaying apoptosis on non-detaching cell culture plates - which plates? (reply: 1)
     
945. How to save my primary cell lines! - (reply: 1)
     
946. does anyone know how to prepare cell extract for Metabolic and Enzymatic Assays - (reply: 6)
     
947. My cells die on poly-d-lysine coated plates! - (reply: 2)
     
948. What is the right shape for NRK cells - (reply: 1)
     
949. isolation of celmembrane of tissue cells - (reply: 2)
     
950. Why fix cells? - Advantages-Disadvantages (reply: 14)
     
951. How to grow frozen DB 3.1 cells - (reply: 1)
     
952. Please recommend a colon cancer cell line - what is the widely used colon cancer cell line? (reply: 3)
     
953. REH cells and knockdowns - (reply: 1)
     
954. cd3- and cd8+ cells - flow cytometry cell population question (reply: 4)
     
955. Cell centrifugation at 3200xg - Can cells survive such speed? (reply: 4)
     
956. confocal microscope, my cells die! - URGENT. (reply: 8)
     
957. mous hepatocyte isolation - (reply: 1)
     
958. transfection with PA-1 cells - (reply: 1)
     
959. Purchase or make competent cells? - (reply: 8)
     
960. How to separate the antigen presenting cell from spleen of rat - (reply: 1)
     
961. need help for cord blood mesenchymal stem cell culture - (reply: 6)
     
962. Transfection of T47D cells with FuGene in 10cm plates - (reply: 2)
     
963. Cell count vs Reference gene - Cell count vs Reference gene (reply: 1)
     
964. Immunofluorescence staining of spheroid cells - (reply: 3)
     
965. Protocol for acridine orange staining of epithelial cells - (reply: 1)
     
966. How small cells can Flow cytometry detect? - Flow cytometry to detect a small cell (reply: 2)
     
967. human cell line - human cell line (reply: 1)
     
968. tg1 and hb2151 cells - (reply: 2)
     
969. serum-starving of cells - (reply: 1)
     
970. How to avoid central cell seeding in 6-well plates - (reply: 19)
     
971. FBS and cell culture - (reply: 9)
     
972. Same cell line but different growth rate - (reply: 4)
     
973. Microplate Bradford with Tissue Whole Cell lysate - (reply: 1)
     
974. Fixing Cells for Flowcytometry - Glutaraldehyde, Formalin, Microwave (reply: 11)
     
975. Punctured with HEK 293 cells - Is there any health risk? - (reply: 2)
     
976. estimating number of cells to seed - (reply: 2)
     
977. How to seed cell in plates - (reply: 10)
     
978. cell dead after changing the medium to PBS buffer - (reply: 3)
     
979. Help! Large vacuoles observed in HEPG2 cell culture! - (reply: 3)
     
980. PC12 cell culture. Do I have to coat plates first? - (reply: 2)
     
981. Serum starving cells - why? - (reply: 4)
     
982. Can scintillation fluid be used to harvest adherent cells? - (reply: 1)
     
983. Do cells transfected with lentivirus express specific surface markers - (reply: 3)
     
984. Difference between cell invasion and cell migration - (reply: 4)
     
985. Dispersing big cell clump growing in flask - Big cell clump in flask (reply: 1)
     
986. too many dead cells why? - (reply: 3)
     
987. Plasmid stability over a 100%? - Why does ampicillin increase cell viability? (reply: 4)
     
988. Do cells transfected with lentivirus express specific unique surface markers - (reply: 2)
     
989. A431 Human epidermal cancer cell line - Good to handle? (reply: 5)
     
990. Help: Cell Lysing - (reply: 5)
     
991. Keep cells metabolically reactive during transport - (reply: 7)
     
992. Avoiding cell clumps - (reply: 7)
     
993. Cells grow on Amp plates but not in LB with Amp - Amp problem, bug strain problem, or ligation problem ? or all of the a (reply: 7)
     
994. can cells just stop having the gene? - (reply: 4)
     
995. Are TC-treated flasks/plates harmful to suspension cells. If so, why? - (reply: 2)
     
996. how important of Serum proportion of culture medium in cell culture - SOS (reply: 8)
     
997. Establishing the hyperlipidimic condition in cell culture - (reply: 4)
     
998. question about dendritic cells and antigen-specific T cells - (reply: 1)
     
999. Baculoviral infection of mosquito cell lines - (reply: 2)
     
1000. CD3 and CD28 T cell stimulation in TUBES - Technical question (reply: 4)
      
1001. How take samples of cultured cells with out disturb monolayer - (reply: 2)
      
1002. cell wall protein extraction of gram-positive - (reply: 1)
      
1003. SL2 cell transfection - (reply: 1)
      
1004. How to do the lysis of cells without white stuff? - (reply: 1)
      
1005. Suspension Cell Culture, Removal of Dead Cells - (reply: 4)
      
1006. cell culture - a beginner - (reply: 3)
      
1007. HL-60 CULTURE - Hematopoietic stem cells (reply: 4)
      
1008. any video or animation of experiments about cell and molecular biology - (reply: 1)
      
1009. How to view the cell treated with bacteria under SEM - (reply: 4)
      
1010. how to calculate cell number? - (reply: 1)
      
1011. Freeze-Thaw competent cells - (reply: 4)
      
1012. Flow Cytometry 2 Parameter Statistics, please help - Can you find standard deviation of % Positive of Total cells based on (reply: 1)
      
1013. bad cell condition for transfection - (reply: 2)
      
1014. cell concentration calculation - (reply: 2)
      
1015. cell concentration calculation - (reply: 2)
      
1016. isolating of mouse spleen Dendritic cells by nycodenz - (reply: 4)
      
1017. size of an endothelial cell - (reply: 2)
      
1018. Suspension cell culture. - (reply: 5)
      
1019. Counting cell numbers - (reply: 6)
      
1020. PC12-Neurite out growth - less no of cells with neurite (reply: 4)
      
1021. Measuring proliferation via cell culture medium - is it possible? (reply: 2)
      
1022. p53 independent cell death - (reply: 2)
      
1023. Cell motility/Invasiveness assays - (reply: 18)
      
1024. Dendritic cell line media - Dendritic cell line media (reply: 1)
      
1025. Cells are not dying when I do the killing curve with G418 - (reply: 5)
      
1026. Assay for cell purity determination - (reply: 2)
      
1027. organelles of a cell - (reply: 5)
      
1028. fungal contamination - fungal in cell culture (reply: 4)
      
1029. Beginning of a cell - (reply: 8)
      
1030. Double-Thymidine block of Hela cells - (reply: 3)
      
1031. Standard cell lines for skin/epidermis - which? (reply: 1)
      
1032. endothelial cell isolation from mouse - using magnetic dynabeads from DYNAL (Invitrogen) (reply: 6)
      
1033. cells and media - (reply: 1)
      
1034. OD600 and cell density correlation? - (reply: 1)
      
1035. how the lentiviral vector transduct into cells? - ask a question (reply: 3)
      
1036. T cell activation via CD3 and/or Cd28 need to solve a problem - T cell activation cd3 (reply: 5)
      
1037. How many plasmid DNA copies per cell? - (reply: 1)
      
1038. Plant cell culture- How do prevent differentiation of callus cells - (reply: 2)
      
1039. cell culture treatment, drug testing - (reply: 2)
      
1040. Confocal microscopy with primary cell cultures - (reply: 8)
      
1041. T Cell Isolation from Peripheral Blood Mononuclear Cells - (reply: 2)
      
1042. stable cell line characterization and validation - (reply: 1)
      
1043. which cyclin to check for a particular phase of cell cycle - (reply: 3)
      
1044. Trouble concentrating cell extract -please help! - (reply: 9)
      
1045. OD and e coli cell density - Pretty basic but I can't find the info (reply: 3)
      
1046. cell synchronization - (reply: 1)
      
1047. nuclear stain for living cell. any recommendation for dye? - (reply: 6)
      
1048. Mycoplasma detection - (reply: 3)
      
1049. Proteinase k treatment of cells - (reply: 2)
      
1050. Can transcriptional regulation research be done in vivo? - No cell line is availbale (reply: 7)
      
1051. Cell lysis and osmotic shock - (reply: 7)
      
1052. making competent SURE cells - (reply: 2)
      
1053. De novo plasmid methylation in eukaryotic cells - (reply: 3)
      
1054. cell line rna extraction - (reply: 1)
      
1055. Collection of selective antibiotic conc for ALL cell lines - Please add yours! (reply: 6)
      
1056. Cell lysis - (reply: 5)
      
1057. endosome markers for mouse T cells - (reply: 3)
      
1058. Suspension cells - (reply: 4)
      
1059. what is the mechanism of transformation of a plasmid into a bacterial cell? - (reply: 5)
      
1060. cell growth and colorimetric assay - (reply: 1)
      
1061. ELISA Question - Finding the concentration of Ab from cell culture (reply: 4)
      
1062. How to maintain pH of designed culture media? - Autoclaving, metabolism of cells cause changes in pH (reply: 2)
      
1063. Lyse cells with TCA - (reply: 1)
      
1064. Another statistics data analysis Q - How to evaluate colony formation from sorted CHO cell (reply: 1)
      
1065. separating normal epithelial cells from malignant transformed - protocol suggestions? (reply: 2)
      
1066. NK92 cell line - (reply: 2)
      
1067. a question about screening transfected cells by GFP protein - (reply: 5)
      
1068. EDTA to weaken cell attachement - (reply: 1)
      
1069. which -20 or -80 is more less degradation of cell lysates - (reply: 4)
      
1070. ccdB competent cells - (reply: 2)
      
1071. Washing during cell staining - FACS (reply: 2)
      
1072. No more apoptosis, no less proliferation but less cells... - (reply: 4)
      
1073. How many days can fiexed cells be left before running flow - (reply: 1)
      
1074. cell line - (reply: 2)
      
1075. Trypsinizing MCF10A cells - (reply: 1)
      
1076. why my BSA contained medium cannot nourish my cells - (reply: 13)
      
1077. transfection in NIH3T3 cells - (reply: 10)
      
1078. Trying to lyse cells to get PCR template - Need something simpler than a miniprep (reply: 10)
      
1079. double digested PCR product into competent cell - (reply: 2)
      
1080. cell culture - FBS (reply: 1)
      
1081. CAn trypsin be used for cell harvest? - (reply: 4)
      
1082. how to count the rounded cells in the well? - (reply: 4)
      
1083. How to make an immortalized cell line/ How to determine IC50? - Protocol for developing a drug resistant cell line? (reply: 6)
      
1084. Cell culture contamination, antibiotic recommendation, any recommd on Normocin - (reply: 7)
      
1085. When are the cells virus free? - (reply: 6)
      
1086. Problem transfecting large plasmid into HEK293 cells - (reply: 8)
      
1087. cell culture contamination - (reply: 2)
      
1088. T cell proliferation/activation markers - CD69, CD38? (reply: 1)
      
1089. Remove BSA from cell culture medium - (reply: 5)
      
1090. cell loss upon centrifugation - (reply: 5)
      
1091. Transfection of Breast Cancer Cell Lines - (reply: 2)
      
1092. Crystal violet and State's cell - (reply: 4)
      
1093. Help! neural stem cells culture - no neurosphere after P1 - Please troubleshoot my protocol (reply: 2)
      
1094. How to tell my cells are contaminated? - (reply: 3)
      
1095. Apply tranfection reagents DIRECTLY onto the cells. - After removing the medium. (reply: 4)
      
1096. Help needed! - HUVEC - C cells - RGD targeting using Human Umbilical Endothelial Cell Line - C (reply: 2)
      
1097. aza treatment of D283 cells - aza treatment of D283 cells (reply: 1)
      
1098. gene expression study - between treated and untreated cells (reply: 2)
      
1099. Mycoplasma Infection! - What to do? (reply: 2)
      
1100. Inconsistency of the cells~~ - (reply: 4)
      
1101. protocol for extract of cell lysat - (reply: 2)
      
1102. Quantifying HeLa cells in a 96 well plate - (reply: 2)
      
1103. MDA cells - (reply: 1)
      
1104. Epithelium cell purification - (reply: 2)
      
1105. How to select a cell line? - (reply: 3)
      
1106. Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
      
1107. Freezing cells in blocking buffer? Urgent! - (reply: 7)
      
1108. Stable cell line stops growing :( - (reply: 6)
      
1109. siRNA transfection in suspended cell lines MV4-11, THP-1 - (reply: 1)
      
1110. siRNA protocol in floating cells - (reply: 1)
      
1111. differetiation of cells - (reply: 2)
      
1112. Extraction of genomic DNA from 10 fold serial dilution of cells. - (reply: 5)
      
1113. cell lysis methods comparison - (reply: 14)
      
1114. Basics of Cell Culture - (reply: 2)
      
1115. Drug Treatment and Cell cycle analysis - (reply: 2)
      
1116. if you plate 40 ul of your electrocomp cells only... - would you get any colonies? (reply: 13)
      
1117. HCT 116 colon carcinoma cells and colon cancer cells in general - feeder layer to increase plating efficiency? (reply: 3)
      
1118. Explant culture - All method of cell isolation coming from animal tissue (reply: 3)
      
1119. treatment of stable cell lines - (reply: 2)
      
1120. E.Coli. XA90 cell - transformation in XA90 cell (reply: 3)
      
1121. How to differentiate Caco2 cells? - Stem cells and differentiation (reply: 1)
      
1122. cells - (reply: 2)
      
1123. my electrocomp cell pop! (arc) - (reply: 10)
      
1124. protocol for purificaiton of human mononucleus cells - (reply: 2)
      
1125. Selection of cell culture media - for HT29 colon cancer cells (reply: 2)
      
1126. How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
      
1127. MTT assay with docetaxel and LNcap cells biphasic growth - (reply: 2)
      
1128. cell membrane - (reply: 6)
      
1129. Adherent cells not adhering - (reply: 3)
      
1130. Endothelial cells purification - (reply: 2)
      
1131. Adherent cells in suspension - (reply: 5)
      
1132. How to make mES cells attch to differentiate? - (reply: 1)
      
1133. Strategy to detect cell survival - for primary cells that can not be trypsinized (reply: 6)
      
1134. linear or supercoiled plasmid for creating stable cell line - (reply: 1)
      
1135. Help: ECM Extraction from cells grown in Alginate gels - (reply: 4)
      
1136. why some microorganisms form filament instead of single cell? - (reply: 1)
      
1137. Studying cell metabolism from frozen tissue samples - Is this even possible? - (reply: 2)
      
1138. can arginine be used as nitric oxide donor compound in cell culture. - kinly help!!!!!!!! (reply: 3)
      
1139. 2 plasmids in one competent cell - is this possible? - Cloning query (reply: 9)
      
1140. concentration of trypan blue... - please help me. (reply: 8)
      
1141. freezing HEK293 cells - (reply: 3)
      
1142. how to determine the length of each phase in cell cycle - (reply: 1)
      
1143. non-transfected cells - (reply: 3)
      
1144. help biochemical pathway ! - lactate effect on mammalian cells (reply: 3)
      
1145. rat1 cells freezing and thawing - how to culture rat1 fibroblasts (reply: 1)
      
1146. Problem with transfection and protein expression - NO DIFFERENCE BETWEEN UNTRANSFECTED AND TRANSFECTED CELLS (reply: 10)
      
1147. diluted cells too much? help - (reply: 1)
      
1148. bEND cells proliferation assay? - (reply: 1)
      
1149. primary cells better than 3T3 cells? - (reply: 3)
      
1150. MCF-7 cells clumped and too small - (reply: 7)
      
1151. Live cell transportation - (reply: 14)
      
1152. Cells showing interesting morphology with treatment - How do I know what's happening (reply: 3)
      
1153. PBS composition for HeLa cells - (reply: 2)
      
1154. where to buy cell lines? - (reply: 3)
      
1155. Flow Cytometry Intracellular and dead cell staining - (reply: 2)
      
1156. effect of TE buffer on cells with cell lysis solution - (reply: 2)
      
1157. Trypan Blue - Multiwell Apoptosis Assay? (reply: 3)
      
1158. single channel and whole cell - (reply: 2)
      
1159. epithelioid cell surface antigen or specific marker - (reply: 2)
      
1160. Funny looking PC12-TrkB cells - (reply: 1)
      
1161. Different sizes of PCR products from different competent cells - (reply: 4)
      
1162. How to break down the plant cell wall? - (reply: 1)
      
1163. Topo TA cloning kit with Top 10 cells - Topo TA cloning kit with Top 10 cells (reply: 4)
      
1164. Cell frozen - (reply: 1)
      
1165. cell culture - (reply: 4)
      
1166. transfecting adherent cells - before adherence? - (reply: 5)
      
1167. AzaC treatment of cells? - (reply: 2)
      
1168. Overexpressing bacterial protein in mammalian cells - which vector to use? - overexpression in mammalian cells (reply: 9)
      
1169. Characterization of a smooth muscle cell line - (reply: 2)
      
1170. typical cell lysate protein concentration - (reply: 6)
      
1171. cell respiration - (reply: 1)
      
1172. Stable cell trasfection questions... - Integration of plasmid... (reply: 5)
      
1173. How to isolate intact mitochondria from live cells? - (reply: 1)
      
1174. Establishing cell lines from brain tumor tissue samples - (reply: 2)
      
1175. Curiosity: competent cell and glycerol - (reply: 7)
      
1176. mammary stem cells - (reply: 1)
      
1177. DNA extraction- bacterial cells - kits - PLease help!!! (reply: 2)
      
1178. ICC of cell on coverslips - losing cells at permeabilization - (reply: 2)
      
1179. Is my RNA dead? - freezing cells after adding RNA lysis buffer (reply: 2)
      
1180. A protocol needed to purify cancer cells from tumor tissue - need help! (reply: 4)
      
1181. mES cells cultured on uncoated dishes/flasks grow well! - (reply: 1)
      
1182. How to disassociate cells for FACS? - (reply: 7)
      
1183. cryopreservation agent missing in frozen cell stock - (reply: 3)
      
1184. Myc tag and endogenous myc - Is there endogenous myc expressed in some fibreblast cells? (reply: 3)
      
1185. transfecting primary cells - (reply: 1)
      
1186. How to keep cells healthy after transfection - Any tips? (reply: 5)
      
1187. Cell Culture Book - any suggestion? (reply: 29)
      
1188. Culture of normal human mammary epithelial cells - (reply: 2)
      
1189. 293F cells revival - (reply: 2)
      
1190. cell culture - (reply: 2)
      
1191. Does TPA induce cell proliferation/growth? - HT1080 cells (reply: 3)
      
1192. Starving cells for stimulation - (reply: 2)
      
1193. protocol on M cell model - (reply: 3)
      
1194. how can I detach infected vero cells from 96 well plate without lose sample? - (reply: 2)
      
1195. expression problems in mammalian cells - (reply: 2)
      
1196. transient transfection of a stable cell line? - (reply: 4)
      
1197. Stable cells die when trypsinized - (reply: 1)
      
1198. I want to inqure some questions about cell lines and cell phenotype - which cell line has the highest transfection efficiency as you know (reply: 1)
      
1199. have you ever used hygromycin to screen transfected HepG2 or gastric cancer cell - all the cells died, why? (reply: 1)
      
1200. WB with cell culture medium - (reply: 2)
      
1201. plasmid purification from mammalian cells? - (reply: 1)
      
1202. plasmid purification from mammalian cells? - (reply: 4)
      
1203. Thp-1 cell culture problems - (reply: 5)
      
1204. help- G0 synchrony of MCF10 A breast cells and MCF-7 cell lines - (reply: 1)
      
1205. stable cell line - how it work? (reply: 4)
      
1206. Get rid of cell debris after transformation? - (reply: 1)
      
1207. HS competent cells in -20 - are they still usable (reply: 2)
      
1208. monocytic cell line differentiation - (reply: 2)
      
1209. serum free cell culture medium - (reply: 6)
      
1210. Obtaining cells from human tissue samples - (reply: 2)
      
1211. Can EDTA remove fibroblast cell? - (reply: 1)
      
1212. Changes on cells. - (reply: 5)
      
1213. The growth behaviour of the transfected cells in hygromycin selection media - (reply: 1)
      
1214. 3T3-L1 cell differentiation - (reply: 2)
      
1215. MCF7 Cell Starvation - (reply: 1)
      
1216. stable cell line questions( cell type matter) - (reply: 2)
      
1217. PI staining of adherent cells in plates for FACS - can this be performed in plates? (reply: 3)
      
1218. Cell Culture with Human Plasma - (reply: 2)
      
1219. good protocol for HeLA cells culture - (reply: 3)
      
1220. cell saparation from lymph nodes and spleen - how can I improve my yield? (reply: 1)
      
1221. How to evaluate Cell viability - (reply: 1)
      
1222. G418 conc for HEK293 cells - (reply: 11)
      
1223. Storage of different cells - (reply: 7)
      
1224. Protein loading control for RAW cell SUPERNATANTS in western blot - western blot (reply: 7)
      
1225. cell lines and apoptosis - (reply: 5)
      
1226. Transient transfection on a stable cell line - (reply: 2)
      
1227. selecting single cell colonies using single cell per well dilution one day after - (reply: 1)
      
1228. Coat Coverslips with Collagen IV/Transfect and Image PC12 Cells - (reply: 1)
      
1229. Protein loading control for RAW cell supernatants - Western blot loading control (reply: 2)
      
1230. Infection efficiency of non-adherent cells - (reply: 3)
      
1231. How to limit fibroblast cell contamination in epithelial cell culture - (reply: 9)
      
1232. Are there TGFB receptors in 293T cells? - ..and if you don't know, how would you find out? (reply: 2)
      
1233. Mycoplasma PCR detection in media/FBS - (reply: 6)
      
1234. Split cells after siRNA transfection? - What to do when cells become confluent after transfection of siRNA (reply: 6)
      
1235. Which medium do you use to grow HEK293 cells? - (reply: 7)
      
1236. PC 12 cell line - (reply: 4)
      
1237. Advantages to eukaryotic cells of having a nucleus? - (reply: 9)
      
1238. detachment of cells - (reply: 5)
      
1239. Contamination of unique cell line (eeek!) - (reply: 5)
      
1240. how to identify cancer stem cell from sp cell?what is its characteristic? - cancer stem cell (reply: 2)
      
1241. how to seperate cells from the dead and the live - (reply: 9)
      
1242. prevent cell attachment - (reply: 2)
      
1243. Expression in other mammalian cells with human H1 promoter - (reply: 1)
      
1244. Cell Banking - cell splittting and banking (reply: 1)
      
1245. Which cell lines are best? - (reply: 6)
      
1246. can antibodies be added to cell culture for 10d before stain - immunocytochemistry (reply: 1)
      
1247. Knockout cell lines? - (reply: 4)
      
1248. Help: Tubulin expression is not the same between my cell clones? - (reply: 1)
      
1249. If I accidentally injecting human breast cancer cell lines into my finger - What happens? (reply: 7)
      
1250. Overlay cell culture medium with oil against evaporation - which oil? (reply: 2)
      
1251. competen cell - (reply: 3)
      
1252. Why EDTA in trypsin? - (reply: 28)
      
1253. WB for comparing the level of certain protein in two different cell lines - (reply: 2)
      
1254. Tumour cells grow slower than normal cells, why? - cell proliferation rate in normal vs tumour cell (reply: 2)
      
1255. Transfection by electroporation of 293 suspension cells - electroporation of 293 cells for large scale transient expression (reply: 2)
      
1256. paraformaldehyde solution - for cell fixation (reply: 19)
      
1257. Making DH10bac competent cells - (reply: 2)
      
1258. How to seed cells evenly in small well plates - (reply: 27)
      
1259. weird DNA bands in the Minipreps; competent cells contamination? - (reply: 3)
      
1260. remedy against cell contamination - (reply: 4)
      
1261. cell culture western blot - (reply: 15)
      
1262. stain similar to neutral red - stain unfixed cells directly (reply: 4)
      
1263. HOW DO YOU STOP CELLS FROM CLUMPING TOGETHER - (reply: 10)
      
1264. How to lift attached CD14 cells? - (reply: 3)
      
1265. Stable transfection of large plasmid into breast cancer cells - (reply: 2)
      
1266. How can i decontaminated my cell culture ? - (reply: 4)
      
1267. Can adherent culture be transformed into suspension cells? - (reply: 4)
      
1268. Cell fixation problem - (reply: 3)
      
1269. effects of CO2 level on mammalian cell culture - (reply: 3)
      
1270. Hela cells and stable transfection help please! - (reply: 6)
      
1271. difference between 293 cells and 293 T cells - (reply: 1)
      
1272. Is cell culture considered in vivo? - (reply: 4)
      
1273. Injection of human cells into mouse mammary fat pad - (reply: 1)
      
1274. Transfecting KG 1 cell - (reply: 3)
      
1275. Normal cell control for cytotoxic screening - (reply: 2)
      
1276. cell stain, nonfluroescent - (reply: 4)
      
1277. getting stable cell lines - (reply: 3)
      
1278. Need to Slow down Cell Culture growth - (reply: 2)
      
1279. making a vector construct and transfecting in smooth muscle cells - (reply: 6)
      
1280. What size needles I should use for cell extraction protein - (reply: 1)
      
1281. Help with protein extraction! - WI-38 cells (reply: 1)
      
1282. Cell adhesion assay - (reply: 2)
      
1283. western - cells treated w/ protein synethesis inhibitor - (reply: 1)
      
1284. Tetracycline in cell culture media - (reply: 5)
      
1285. rhG-CSF assay using NFS-60 cell line - (reply: 14)
      
1286. Treatment of cancer cell - (reply: 2)
      
1287. Loss of cell viability in primary culture - (reply: 4)
      
1288. why centrifuge before doing a cell count? - (reply: 1)
      
1289. Please recommend a cell line that express high level of ER alpha or beta - (reply: 3)
      
1290. amount of proteins isolated from cells - (reply: 2)
      
1291. What s the maximum allowable limit of DMSO as a solvent in cell culture - (reply: 8)
      
1292. clumping cells - s. aureus wildtyp cells (reply: 3)
      
1293. whole cell extracts - (reply: 1)
      
1294. Primary Hippocampal cell culture-DEAD! - (reply: 3)
      
1295. Culturing Hep G2 Cells - (reply: 4)
      
1296. How long can cells survive at 4 degree - (reply: 2)
      
1297. Isolation of langerhans cells - (reply: 1)
      
1298. Medium for adherent cells and serum/protein free - (reply: 2)
      
1299. ORL-1 overexpression in SH-SY5Y cell line - (reply: 1)
      
1300. Mouse ES cell won't attach - (reply: 1)
      
1301. determine cyp3a16 primer - mouse liver progenitor cell (reply: 5)
      
1302. monitor cell growth - (reply: 1)
      
1303. Competant cells & incompetant scientist - No colonies formed (reply: 8)
      
1304. RNA extraction from formaldehide fixed cells - (reply: 2)
      
1305. New stock of AD-293 cells - (reply: 1)
      
1306. vector for expression of tag on cell surface - (reply: 7)
      
1307. Selection Problems with G418 - CaPO4 Transfection and single cell picking (reply: 4)
      
1308. Fibroblats contamination in primary cell cultures - Fibroblats contamination in primary cell cultures (reply: 6)
      
1309. proteasomal inhibition and cell death? - (reply: 1)
      
1310. why competent cells take up only 1 type of plasmids? - (reply: 7)
      
1311. Help: bFGF or EGF for mouse neural stem cells - (reply: 2)
      
1312. cell lysis buffer recipe - simple question (reply: 6)
      
1313. Sk-Br-3 cells - (reply: 5)
      
1314. Help with HeLa cells culture... - (reply: 10)
      
1315. Obtaining single cell suspension from fixed biopsy tissue - (reply: 2)
      
1316. FACSing of MCF-7 cells - (reply: 3)
      
1317. transfection in MDAMB231 cells - (reply: 2)
      
1318. Transfecting primary cells (imr90) - PROBLEM - (reply: 4)
      
1319. Why tumor cells are hard to culture - (reply: 3)
      
1320. Why my cells dont grow - hippocampal cell (reply: 5)
      
1321. Electroporation of stem cells with DNA in Top10 - endotoxins? - (reply: 4)
      
1322. PMSF and tissue culture - Can you apply PMSF purified proteins to cultured cells? (reply: 2)
      
1323. magnetic affect of ameoba cells? - how can i make this a science fair project (reply: 1)
      
1324. treat cells with progesterone and estrogen - (reply: 1)
      
1325. Evaluation of Boyden Chamber Assay - how to count cells? (reply: 4)
      
1326. difference between treating cells with ATP disodium salt and ATP magnesium salt - (reply: 2)
      
1327. Cell Fixation - Ethanol or Methanol? - (reply: 4)
      
1328. cell lysis buffer for membrane protein please? - desperately need help.... :S (reply: 8)
      
1329. C6 Cells Contaminated - What do you think it is? (reply: 6)
      
1330. growth rate of SK-BR-3 cells - (reply: 3)
      
1331. hippocampal cell culture - I need a help (reply: 5)
      
1332. neural stem cell markers? - (reply: 2)
      
1333. How to make electrocompetent cells without refrigerated centrifugue - (reply: 3)
      
1334. G418-selected cells do not divide - (reply: 8)
      
1335. Bacterial cell adhesion assay - (reply: 6)
      
1336. unwanted cell clumping after splitting MCF7 cells - (reply: 5)
      
1337. T cell culture - ag specific T cell culture (reply: 1)
      
1338. hematopoietic stem cell trans-differentiation - (reply: 4)
      
1339. ChIP from low amount of cells - (reply: 1)
      
1340. T cell culture - (reply: 2)
      
1341. What's difference between RPMI and DMEM - which media I should use to culture cells after transfection? (reply: 3)
      
1342. differentiating live from dead bacterial cells - (reply: 2)
      
1343. primary cortical neuronal culture--how to avoid cell clumping? - (reply: 1)
      
1344. Long-time storage of tissues/cells with fluorescent tracers - (reply: 4)
      
1345. MDCK cells for barrier tests - Who has experience? (reply: 2)
      
1346. Transfection of 293 cells for protein expression - optimum conditions for transfection!!! (reply: 5)
      
1347. cells stained with NF-KB and DAPI - (reply: 11)
      
1348. Why is non-viral transfection efficiency of endothelial cells low? - (reply: 1)
      
1349. Protein level in fibroblasts cell line - (reply: 1)
      
1350. Hepg2 cells resistant to trypsin digestion - (reply: 7)
      
1351. vector for stem cell differentiation - (reply: 2)
      
1352. Immunofluorescence of frozen cell culture inserts - (reply: 6)
      
1353. I have a simple question about cell ? - (reply: 5)
      
1354. T cell proliferation assay - (reply: 1)
      
1355. mycoplasma contamination and MEFs - (reply: 2)
      
1356. Stem Cell growth on soft agar - (reply: 3)
      
1357. SHSY5Y cell transfection - (reply: 5)
      
1358. Cell transfection - TransIT vs Fugen - (reply: 3)
      
1359. what is the function of the morpholinopropanesulfonic acid? - cell labeling (reply: 4)
      
1360. Debris or particles in medium - A549 cells and weird particles (reply: 4)
      
1361. mold problems in cell culture - (reply: 1)
      
1362. far western - Would the expression of the protein in mammalian cells be enough for far wester (reply: 2)
      
1363. Progenitor cell type - (reply: 1)
      
1364. Cell surface antigens - identification (reply: 8)
      
1365. can the phoenix cell be transfected using lipofectamine ? - (reply: 2)
      
1366. How long can the whole cell extract for ChIP stay in -80C? - (reply: 1)
      
1367. Increase in cell viability? - (reply: 1)
      
1368. Overexpression of Viral Protein in mammalian cell line - (reply: 3)
      
1369. Making frozen cell pellets competent - (reply: 3)
      
1370. Chemotaxis Assay for Primary Mouse Peritoneal Cells - (reply: 7)
      
1371. Unhealthy Passaging? - passaging cells too much... will they go bad? (reply: 4)
      
1372. Getting rid of the dead cells during selection of transfected colonies? - (reply: 2)
      
1373. Dead cell removal -why? - (reply: 3)
      
1374. optimizing selection of transfected cells - (reply: 2)
      
1375. cancer cell transfections - (reply: 2)
      
1376. MTS assay of MCF7 and MDA-MB-453 cells - (reply: 1)
      
1377. About the cell lysis methods - (reply: 8)
      
1378. primary cell culture death - (reply: 8)
      
1379. Need prototocol for membrane fractionation from mammalian cells - (reply: 1)
      
1380. ELISA IL12 p70 from mouse dendritic cells culture SN - (reply: 2)
      
1381. Culturing of HEP G2 cells - (reply: 5)
      
1382. new lab, cells die after passaging - general fish cell culture methods (reply: 5)
      
1383. low viability of stably transfected cell line from cryostock - (reply: 1)
      
1384. Fibroblast cell line - (reply: 2)
      
1385. Can I use 3H-Thymidine on adhesive cell for cell proliferation assay - (reply: 8)
      
1386. FBS or FCS for NIH3T3 cells - How to grow these cells optimal (reply: 7)
      
1387. How 2 Calculate cells/mL to cells needed per well? - EZ way to determine how much cell suspension to add/well for a certain number of (reply: 6)
      
1388. Cell density to platte after trypsinization - (reply: 3)
      
1389. lipid content HepG2 cells - (reply: 2)
      
1390. 3T3 cells and lysis buffer recepie - western blot (reply: 1)
      
1391. M-NFS60 cell line revival - Revival protocol (reply: 3)
      
1392. Starvation of cells for higher uptake? - (reply: 2)
      
1393. Any effect of different culture dishe size on cell? - (reply: 20)
      
1394. mouse spleen cell culture - (reply: 2)
      
1395. doubling rate of MDA-MB-453 cells - (reply: 1)
      
1396. a question about splitting MCF-7 cells - (reply: 5)
      
1397. Primary endothelial cells went differentiated - Medium, serum or other factors? (reply: 6)
      
1398. Trypan blue cell death assay on cardiac myocytes - (reply: 3)
      
1399. Monoclonal antibody production - no cells show high O.D. - (reply: 8)
      
1400. What is an acceptable CO2% range for growing cells in DMEM? - (reply: 8)
      
1401. How to do Per cell RNA concentration? - (reply: 2)
      
1402. mycoplasma effects on cells - will mycoplasma kill the cells? (reply: 2)
      
1403. Cells after thawing - (reply: 6)
      
1404. method for staining of invisible cell pellets? - (reply: 1)
      
1405. what happens when a cell gets frozen without cryopreservative - can polysomes and RNA be extracted from such cells? (reply: 4)
      
1406. rna isolation from bone marrow cells - (reply: 1)
      
1407. Has any one successfully decontaminated a contaminated cell culture - (reply: 3)
      
1408. Monocyte-derived DC generation: CD14 separation or adherent cells better? - (reply: 1)
      
1409. 293 cells sonication - How do I do it???? (reply: 3)
      
1410. GFP expressing cells - masking of GFP expressing cells!!! (reply: 6)
      
1411. Low transfection of siRNA into HaCaT cells. Please help - (reply: 1)
      
1412. transplantable rats or mice cell line in nude mice - (reply: 1)
      
1413. preparation of electrocompetant cells. - what about the density? (reply: 3)
      
1414. How to tell cell are contaminated by fungi - (reply: 4)
      
1415. can i still see the GFP reaction after the distruction of cell. - (reply: 1)
      
1416. Need protocol: CFSE Staining/ primary cells - (reply: 2)
      
1417. How to dispose of cells grown in MM with ammonium chloride? - (reply: 1)
      
1418. why do you prepare heat-stable fractions of cells? - (reply: 1)
      
1419. Cell Lines from Tumor Tissue Samples - Advice/Protocols on best techniques (reply: 2)
      
1420. Puromycin and HEK293 cells - (reply: 3)
      
1421. Imaging cells in matrigel - how to stain ? Prepare ? (reply: 1)
      
1422. neuronal markers for the mature level for spinal cord cell - (reply: 4)
      
1423. Why HepG2 and HUH7 - for hepatitis cell culture matter. (reply: 3)
      
1424. tissue n cell culture - (reply: 3)
      
1425. how to calculate number of cells from OD? - (reply: 2)
      
1426. do cells benefit from being of different age? - (reply: 2)
      
1427. SK BR-3 human breast cancer cells. - Is it Bcl-2 negative or positive. (reply: 3)
      
1428. cell culture contamination - (reply: 2)
      
1429. Slow cell growth when thawing cells - (reply: 7)
      
1430. Can I call my cell a established cell line? - (reply: 1)
      
1431. when to start counting passage number for primary cells? - Bovine aorta endothelial cells, for example (reply: 2)
      
1432. how long can cell lines be carried? - (reply: 5)
      
1433. problems digesting genomic DNA from cell lines - (reply: 1)
      
1434. Could we freeze the 0.25 trypsin EDTA and thaw it, use it? - (reply: 8)
      
1435. mycoplasma contamination through liquid nitrogen storage - (reply: 6)
      
1436. transfection of HeLa cells - (reply: 4)
      
1437. ES cells and feeders - (reply: 1)
      
1438. cell stain and the fixation? - (reply: 2)
      
1439. I cannot get protein from my stable transfection cell - (reply: 4)
      
1440. Cell Differentiation - Time periods and environment for proper cell differentiation (reply: 1)
      
1441. what determines wheather a virus can infect a host cell - (reply: 3)
      
1442. Cell Lysis - (reply: 2)
      
1443. Inducible shRNA knockdown in primary cells - (reply: 1)
      
1444. Does anybody have MIN6 cells? - MIN6 Cells (reply: 1)
      
1445. PC3 Cells & DU145 Cells - Cell Line (reply: 2)
      
1446. SiRNA and GFP transfection MCF7 cells - (reply: 4)
      
1447. Expression of fusion protein in mammalian cells - (reply: 2)
      
1448. questions about MCF-7 cells - (reply: 10)
      
1449. Question on Overgrown Cell Culture - (reply: 9)
      
1450. detachment of my cells?/ - (reply: 7)
      
1451. How long it take for breaking cell by a water bath sonication - (reply: 4)
      
1452. depolymerizing microtubules in 293 cells - (reply: 1)
      
1453. making stable clones through single cell dilution after transfection - (reply: 2)
      
1454. cell proliferation assays - (reply: 2)
      
1455. How to show a good control for cell treatment - (reply: 14)
      
1456. Retroviral transduction of cells - troubleshooting help please! - (reply: 8)
      
1457. Competent Cells Preparation - (reply: 9)
      
1458. After cells are treated with TCA, how to resolve the protein in cell pellet? - (reply: 4)
      
1459. Maintaining cells without freezing - (reply: 5)
      
1460. should dead cell detach? - (reply: 2)
      
1461. cell growth rate, model - (reply: 3)
      
1462. how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
      
1463. How to treat total bacterial cell DNA with RNase - I need to get RNA free DNA (reply: 6)
      
1464. Stem Leydig cells - (reply: 1)
      
1465. HepG2 cells, ideal passage number for western blot - (reply: 1)
      
1466. adenovirus vs. retrovirus cell infection--pros and cons? - infecting PC12 cells and overall general toxicity (reply: 3)
      
1467. hybridoma cell line - (reply: 1)
      
1468. problems with Clonetech 10G cells - (reply: 1)
      
1469. No p53 in the HEK293 cells on my IF - Fluorescence microscopy (reply: 4)
      
1470. THP-1 cell line - Passage number affect phagocytosis (reply: 12)
      
1471. Colony PCR was carried out following lysis of cells with Igepal CA - 630 .. - But how? (reply: 2)
      
1472. MTT test for the suspension culture - Raji cell and K562 (reply: 4)
      
1473. stable line making of adherent cells - concentration of puromycin for adherent cells (reply: 3)
      
1474. maximum plasmids can be co-transfected into Hela cells - (reply: 4)
      
1475. keep beating of cardimyocyte cells? - (reply: 13)
      
1476. T cell proliferation assay - (reply: 3)
      
1477. thawing of fridged cell culture - Reviving of cells (reply: 2)
      
1478. Culture of adherent SH-Sy5y Neuroblastoma Cell Line - (reply: 5)
      
1479. Starving the cells - (reply: 2)
      
1480. CCR5 cytometry on dendritic cells, probles with isotype control - (reply: 2)
      
1481. HL-60 retrovirus infection - retrovirus infection system for HL-60 cells (reply: 5)
      
1482. lymphocytes and hepatocytes are the same!? - (reply: 4)
      
1483. transformation of competent cells with recombinant plasmids - (reply: 2)
      
1484. transfection of MCF7 cells - (reply: 2)
      
1485. plasmids smaller than empty vector - using expensive cells - still not working (reply: 1)
      
1486. Has anyone transfected ST15A cells? - Transfection of ST15A cells? (reply: 3)
      
1487. Knocking down the cPLA2,sPLA2 in Cancer cells - (reply: 3)
      
1488. cell harvest by scraping versus trypsinization - (reply: 21)
      
1489. Primary cell cutures - what is this (reply: 2)
      
1490. ethanol/IPA kill cell - (reply: 1)
      
1491. chemical that impede cell growth - (reply: 1)
      
1492. Cell culture Contamination - Again! (reply: 13)
      
1493. EM, cell preparation: to coat or not to coat slide with collagen - electron microscopy (reply: 1)
      
1494. PBS and Cells - How long can cells sit in PBS? (reply: 6)
      
1495. microglia cell culture - (reply: 5)
      
1496. Freeze/Thaw procedure in cell lysis - How is this done??? (reply: 12)
      
1497. siRNA delivery in non-dividing cells - (reply: 8)
      
1498. cell death during transfection - (reply: 3)
      
1499. PC12 cell culture - (reply: 5)
      
1500. Best way to detect shRNA produced in cells? - (reply: 2)
      
1501. Turbo Competent Cells - (reply: 3)
      
1502. flow cytometry - labelling surface receptor on monolayer cells (reply: 5)
      
1503. yeast contamination in my cell culture - (reply: 11)
      
1504. PMA activation of cells - (reply: 5)
      
1505. Cell Biologists working in 3d cell cultures - (reply: 1)
      
1506. losing green light form pIRES2-EGFP transfected cells - (reply: 13)
      
1507. How to identify whether a cell line might posses stem cell characters - Any general molecular markers for stem cells? (reply: 4)
      
1508. Help, Neuroscience cell lines - (reply: 1)
      
1509. Use of antibiotics in cell culture? - (reply: 5)
      
1510. How to trace siRNA in cells - (reply: 4)
      
1511. how to prepare total cell lysate for Western blot? - (reply: 10)
      
1512. stability of hybridoma cell lines - how to produce totally stable hybridoma cel line? (reply: 3)
      
1513. doxorubicin treated cells - (reply: 1)
      
1514. Impact of dead cells in a culture? - When cells die, how toxic can they become to viable cells? (reply: 4)
      
1515. Problem with cell line/G418 - (reply: 8)
      
1516. Removing adherent cells for Flow cytometry without trypsin - how to do it (reply: 3)
      
1517. Chondrocytes - Need a Chondrocyte cell line (reply: 2)
      
1518. Glioblastoma cell lines and transfection - stable transfection (reply: 5)
      
1519. RNA isolation from trypsinized cells - (reply: 4)
      
1520. Problem establishing a stable PC12 cell line - (reply: 6)
      
1521. how to solve the problem cell culture contamination? - (reply: 2)
      
1522. How to make 3T3 mouse fibroblast cell bank? - (reply: 1)
      
1523. partial proteolysis by trypsin - (reply: 2)
      
1524. how to know that ur cells are confluent? - (reply: 2)
      
1525. Slow growing cells (MCF-7 & MDA-MB231) - (reply: 7)
      
1526. How to make a stable cell line? Help! - (reply: 3)
      
1527. Good book on cell culture - (reply: 5)
      
1528. time when cells go into apoptosis - (reply: 2)
      
1529. Harvest of cells from 96 well plates for Western blots - (reply: 8)
      
1530. doxorubicin treated cell cutlures? - (reply: 1)
      
1531. before transfection into mammalian cells first transfect into E-coli.... - (reply: 8)
      
1532. Cell line passage number? - (reply: 3)
      
1533. Please help! Can Dh5alpha Competent cells be used for electroporation? - (reply: 8)
      
1534. basic techniques for cell treatment - (reply: 6)
      
1535. mouse liver perfusion and hepatocyte isolation - Is perfusion absolutely necessary? (reply: 4)
      
1536. Trypsin-Free Detachment of cells using EDTA - (reply: 10)
      
1537. Protein didn't express - why? - M15 E. coli culture and pQE30UA vector; pTrcHis2TOPO and TOP10 E. coli cells (reply: 10)
      
1538. The mechanism of the cell "fix" reagent? - (reply: 1)
      
1539. sodium azide on living cells? - (reply: 3)
      
1540. adding antibiotics to cho cell media - (reply: 2)
      
1541. Whole cell extraction buffer... - does any one have the recipe? (reply: 1)
      
1542. Can the monocytic cell line MD (ATTC no. CRL-9850) be differentiated into dendri - (reply: 1)
      
1543. Fetal bovine serum and cell culture - (reply: 5)
      
1544. ELISA with fixed bacterial cells - (reply: 1)
      
1545. optimization of cell isolation protocol - optimization of cell isolation protocol (reply: 1)
      
1546. cells not adhering to flask after thawing - (reply: 14)
      
1547. Why starving cells before a tratment? - (reply: 6)
      
1548. obtaining cell clone? - (reply: 3)
      
1549. cell death pathways, how many? - and their basic differences.... (reply: 6)
      
1550. establishment of stable cell line - (reply: 2)
      
1551. Measuring cell viability via luminescence... plate type? - Promega CellTiter-Glo kit (reply: 2)
      
1552. cell cetrifugation_how fast can you go? - (reply: 6)
      
1553. Functional study with PC12 cells - (reply: 16)
      
1554. What's the maximum amount of DMSO you can use in cell culture? - (reply: 4)
      
1555. lysis of cells - how to lyse cells (reply: 6)
      
1556. how to avoid MEF when mES cells are transfected by lentivirus? - (reply: 4)
      
1557. Cell line to be used for protein expression an purification - (reply: 8)
      
1558. TB1 Competenet Cell - (reply: 2)
      
1559. TLR expression on Vero cells - (reply: 2)
      
1560. 293 cell lines - (reply: 4)
      
1561. Role of DMSO in Cell fusion. - (reply: 2)
      
1562. Growing PC12 cells on glass - (reply: 3)
      
1563. Need protocol for mouse ES cell stable transfection - (reply: 1)
      
1564. how to set up a stable cell line? - why false positive always appears? (reply: 2)
      
1565. does phenol:chloroform extracted plasmid creats problem in Mammalian cell cultur - (reply: 5)
      
1566. RNA extraction from cells grown in matrigel - (reply: 1)
      
1567. in vitro cell proliferation assay - use of the DMSO as drug solvents (very urgent) (reply: 3)
      
1568. Is there a quicker way of making stable cell line? - (reply: 7)
      
1569. Are transiently transfected DNA methylated in mammalian cells? - (reply: 3)
      
1570. How to deplete calcium from cell medium? - (reply: 1)
      
1571. why transfected cells has less RNA? - (reply: 2)
      
1572. Is competent cell still good in -80oC for 16 months? - got a different plasmid after plasmid prep (reply: 3)
      
1573. methods of inducing stress in cell culture - (reply: 8)
      
1574. cell separation by centrifugation - (reply: 1)
      
1575. Transfectiion of H295R cells by Fugene6 - (reply: 8)
      
1576. cell line nonresponsiveness - is it a time to change my cell line???? (reply: 2)
      
1577. Mammalian Cell adhesion and proliferation on biomaterials - (reply: 1)
      
1578. Cell cycle progression and arrest - (reply: 1)
      
1579. removal carbohydrates cell surfaces - how to "shave off" sugars from cell surfaces (reply: 1)
      
1580. Cheap and quick cell viability assay - (reply: 11)
      
1581. Measuring receptor expression by FACS - interference with trypsin exposure? (reply: 2)
      
1582. siRNA transfection of MIN6 cells - (reply: 2)
      
1583. Agarose gel electrophoresis - for separating bacteria cells?! (reply: 5)
      
1584. Is it possible to harvest E.coli cells for purification of DNA from LB agar and - (reply: 5)
      
1585. Long-term cell storage at -80C - (reply: 7)
      
1586. Cells survived after G418 selection are transfected cells? - (reply: 2)
      
1587. Storage of cell samples before flow cytometry - (reply: 3)
      
1588. Saos-2 cells transfection - (reply: 1)
      
1589. macrophage cell line - problem with the media conditions (reply: 5)
      
1590. how to make neutrophil cell lysates for Western blot? - (reply: 3)
      
1591. How do you select for stable transfectants in a suspension cell line? Soft agar - (reply: 2)
      
1592. getting rid of shaky hands during cell culture/pipetting - (reply: 9)
      
1593. cell line selection? - suggestions (reply: 6)
      
1594. Mouse endothelial cell line - (reply: 1)
      
1595. number of 293T cells at 60-70% confluency - (reply: 4)
      
1596. trypsin peptide extraction storage - (reply: 3)
      
1597. Cell cycle analysis (FACS) analysis and GFP detection - (reply: 10)
      
1598. infected cell line? - fungal contamination? (reply: 5)
      
1599. commercially available cell lines? - (reply: 2)
      
1600. Growing cell lines from frozen tissue samples - Is this even possible??? (reply: 8)
      
1601. RNA extraction from small cell number - (reply: 4)
      
1602. Cell Growth - Help! (reply: 7)
      
1603. Cells not growing - (reply: 5)
      
1604. Co-IP experiments, Ratio of amount of cells/lysis volume/ug antibodies - (reply: 2)
      
1605. Why use fetal calf serum for cell culture? - Why not use Fetal pig serum? (reply: 15)
      
1606. Trypsin:EDTA cell detachment process - (reply: 6)
      
1607. Cell Culture Microscope - Microscopes (reply: 4)
      
1608. Trypan Blue exclusion - (reply: 2)
      
1609. M17 cell transfection - (reply: 1)
      
1610. Cell lysing for western blot (Help!) - (reply: 2)
      
1611. Stem cell tranfsfection - Electroporation or ca-phos precipitated DNA? (reply: 7)
      
1612. retroviral transfection of pheonix cells - (reply: 3)
      
1613. Cell Viability VS Cell Proliferation assays - (reply: 2)
      
1614. how to build a stable transfection in a suspension cell - (reply: 1)
      
1615. crude cell lysate - storage of crude cell lysate (reply: 3)
      
1616. How to culture stable cell lines? - (reply: 3)
      
1617. CHIP - Harvesting cells by trypsin - (reply: 1)
      
1618. effect of enzymes on cell membrane - (reply: 4)
      
1619. Cell passage nomenclature - (reply: 4)
      
1620. confocal assay for FITC-labled peptide in Hela cell - (reply: 1)
      
1621. Can DH5α be the host cell of the PCR-XL-TOPO from Invitrogen? - Help me (reply: 4)
      
1622. Primary culture hepatocytes of mouse - (reply: 2)
      
1623. subculture primary renal tubular epithelial cell - (reply: 7)
      
1624. Techniques for counting apoptotic cells - (reply: 6)
      
1625. Whole cell vs. supernatant - (reply: 4)
      
1626. Persistent cell culture contamination by fungi and bacteria - Cell culture, cell biology (reply: 6)
      
1627. Types of serum for stem cell growth - (reply: 1)
      
1628. Adult stem cell differentiation - (reply: 2)
      
1629. possible contamination of a cell culture? - (reply: 3)
      
1630. Cell Lysates for western - (reply: 4)
      
1631. Normalizing Western Blot to cell number without cell counting - (reply: 5)
      
1632. transfecting DNA into Jurkat cell - (reply: 1)
      
1633. Treating cells with oil - (reply: 5)
      
1634. single cells from human tissue? - need protocols (reply: 1)
      
1635. Immunocytochemistry on cells that grow in suspention - is it possible? (reply: 6)
      
1636. Tryphan Blue staining for cell viability - (reply: 1)
      
1637. SDS-PAGE for A-beta proteins from cell lysate - (reply: 6)
      
1638. Anyone has experience to grow sespension 293 cells? - (reply: 1)
      
1639. cells with inserts vs cells - (reply: 3)
      
1640. Cornea Epithelial Cell culture - (reply: 1)
      
1641. GM-CSF-secreting cell line - (reply: 1)
      
1642. what kind of adherent cell lines can be analyzed by FCM through Annexin V/PI sta - (reply: 2)
      
1643. HELP: HEK293T cells and geneticin (G418) - (reply: 1)
      
1644. cell cycle data normalization - (reply: 1)
      
1645. Did anyone here use neomycin to kill MCF-7 cells? - (reply: 4)
      
1646. Why are lysosomes considered the cell's "cleanup crew"? - i need help (reply: 1)
      
1647. How to make the neural stem cell differentiate ? - Neurospheres from mouse E14 (reply: 1)
      
1648. Caco-2 Vs Hybridoma cells - contamination of Caco-2 cell line with hybridoma cell line (reply: 3)
      
1649. nice all i have a problem about cell "memory" - (reply: 2)
      
1650. Can I extract nuclear proteins in transfected cells? - (reply: 2)
      
1651. How to prevent cells from detaching during immunofluorescence? - how to keep them on the glass coverslip? (reply: 1)
      
1652. Ordering CHO-K1 cells - (reply: 1)
      
1653. What is the transport mechanism of lipophilic drugs/agents into a cell? - Cell Membrane & Apoptosis (reply: 3)
      
1654. why my IFN gamma treated cells still high in TER? - (reply: 1)
      
1655. Urgent ! Help for infection ... - Many dark spots appear beside my cell (reply: 3)
      
1656. co-transfection in NRK cells - (reply: 1)
      
1657. Cells Detach With Serum Free Media - (reply: 5)
      
1658. 293T cell line, how to prevent cell clusters? - (reply: 1)
      
1659. Re: cell number - (reply: 1)
      
1660. Cell cycle analysis - Include floating cells or not?? (reply: 7)
      
1661. How to increase protein expression level in cell lines - (reply: 2)
      
1662. Death of Cells After Defrosting - MDBK cells not surviving (reply: 2)
      
1663. hypoxic cells - how to make hypoxic cells??? (reply: 2)
      
1664. Extensive cell death after transfection in a 24-well plate - (reply: 3)
      
1665. competent cell - (reply: 2)
      
1666. Hela cells clumping in center of well of 96well plate. - (reply: 1)
      
1667. Cell Signaling - (reply: 3)
      
1668. mouse B cells - (reply: 1)
      
1669. Loosing Cells with centrifugation? - (reply: 3)
      
1670. reasons for apoptosis in primary cell culture? - (reply: 2)
      
1671. What is splitting cells and how? - (reply: 2)
      
1672. Problems with cell lysate - (reply: 4)
      
1673. Glycerol wash protocol for chemical competent cells - (reply: 1)
      
1674. competent cells - making compentent cells (reply: 2)
      
1675. help!-- plant cell culture - (reply: 1)
      
1676. Establishment of human embryonic stem cell - (reply: 3)
      
1677. stable cell line selection - (reply: 4)
      
1678. Help: got the stable cell line without my target gene - (reply: 3)
      
1679. TEM of langerhans cells - (reply: 1)
      
1680. Sticky cells - (reply: 3)
      
1681. Co-transfection for stable cell lines - mammalian - Need to know the ratio of the plasmids that need to be used (reply: 2)
      
1682. ask for 721.221 cell line - (reply: 3)
      
1683. Anyone tranfected macrophage cells before? - (reply: 3)
      
1684. Spectrophotomery of cell 550 vs. 600 - spectrophotometry for cells (reply: 2)
      
1685. Splitting transfected cells: do they still overexpress my gene of interest ? - (reply: 5)
      
1686. extract peptides from tissue/cell culture for ELISA kits - (reply: 3)
      
1687. T cells in immune system - (reply: 1)
      
1688. looking for a good siRNA transfection reagent for 3T3 L1 cells ? - (reply: 5)
      
1689. T cell activation - (reply: 6)
      
1690. endothelial cells transfection - (reply: 2)
      
1691. transfection of M17 cells - (reply: 4)
      
1692. How many passages cells lines can survive before senescence? - (reply: 8)
      
1693. expressing a gene in a cell HELP - (reply: 1)
      
1694. RNA extraction from small number of cells - (reply: 3)
      
1695. siRNA in non-adherent cell lines? - Tips please (reply: 4)
      
1696. NMDA tox and Rat Cerebellar Granular Cells ! - NMDA tox assay for Rat CGC's ???? (reply: 3)
      
1697. Counting MCF-7 cells - (reply: 4)
      
1698. Host cells for pBELOBAC11 - (reply: 4)
      
1699. How long DNA and siRNA stay in transfected cells - (reply: 2)
      
1700. URGENT-Selecting for transformed bacteria cells - (reply: 1)
      
1701. Stable transfectants of HEK293 (selection with G418). Question about splitting ( - Cells growing confluent long before G418 kills them, is splitting smart or not? (reply: 9)
      
1702. mammalian cells invaded by intracellular microbes - how can I decontaminate? (reply: 3)
      
1703. Red blood cell in distilled water. - (reply: 5)
      
1704. RAW 264.7 cell line transfection - (reply: 1)
      
1705. What are the morphologic features of cells undergoing dedifferentiation - (reply: 2)
      
1706. change adherent cells into suspenion cells - (reply: 1)
      
1707. general cell culture (and IMR90 line) - any resource to find out info about lines (reply: 1)
      
1708. detection of GFP fluorescence in whole cell bacteria - (reply: 1)
      
1709. serum starving cells - (reply: 1)
      
1710. How to fix and stain lymphoblastoid cell onto glass slides - (reply: 5)
      
1711. What type of cell culture contamination is this? - large pieces of jelly-like material!! (reply: 7)
      
1712. no growth on plate agar for competent cell - (reply: 6)
      
1713. help me for Dual-Luciferase Assay in Jurkart Cells - (reply: 6)
      
1714. starvation medium recipe for Hela cells - (reply: 2)
      
1715. Counting and sizing cells - (reply: 1)
      
1716. Inturpreting cryopreservation data - I need help applying data I'm colleting with the freezing of E. coli cells t (reply: 1)
      
1717. There's got to be a better way to count cells - (reply: 6)
      
1718. transiently transfected vectors replication - do they replicate in an eukaryotic cell? (reply: 1)
      
1719. Transfection into a cell line which already express your protein - (reply: 3)
      
1720. siRNA stable clone problem - protein level not reduced, but did have impact on cell function! (reply: 2)
      
1721. Isolation of episomal plasmid DNA from mammalian cells - (reply: 1)
      
1722. problem transfection Hela cells - (reply: 8)
      
1723. trypsin/EDTA passaging problem - (reply: 3)
      
1724. How to study the viability of cells - (reply: 10)
      
1725. MCF-7 Cell line - (reply: 6)
      
1726. cell lysis? - (reply: 2)
      
1727. Switching CHO cells media from high to low glucose - how would that effect cells? (reply: 1)
      
1728. cell death after subculture - (reply: 3)
      
1729. live cell confocal microscopy - protocol please? (reply: 2)
      
1730. can you introduce mutation directly into the cells? - other than just transfecting it with a vector... (reply: 1)
      
1731. Will low concentration of ethanol affect cell line? - (reply: 1)
      
1732. Mystery - Cell Proliferation Assay - (reply: 6)
      
1733. Isolation and culture of white blood cells. - (reply: 1)
      
1734. Help with Cell attachment problem - (reply: 3)
      
1735. Neuro2A cell culture Death and Debris - Problem keeping cells alive for more than a week. (reply: 2)
      
1736. How many cells to lyse for Western? - Decreasing signal in my Western Blot (reply: 2)
      
1737. Which Mycoplasma detecting kit is the best? - Difficult to choose, give me an advice (reply: 2)
      
1738. Human Neural Stem Cells wanted - (reply: 1)
      
1739. Qustions about stem cell culture - (reply: 1)
      
1740. plating cells for transfection - (reply: 2)
      
1741. Why Stem cell need beta-mecaptoethanol - (reply: 1)
      
1742. cytotoxicity and cell proliferation assay - preference of dyes for cell viability (reply: 2)
      
1743. How to make a stable cell line which is easily differentiated - (reply: 3)
      
1744. protocol for electrocompetent cells preparation - (reply: 5)
      
1745. Basic cell splitting help - (reply: 4)
      
1746. Transient transfection on a stable cell line - (reply: 6)
      
1747. Killing of cells via the membrane - (reply: 4)
      
1748. especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
      
1749. Antibody with sodiem azide added to mammalian cells - (reply: 2)
      
1750. need help for culture protacal of new stem cell - (reply: 5)
      
1751. Geneticly modified cancer cells - (reply: 2)
      
1752. Activating human T cells - (reply: 8)
      
1753. the step for staining bacteria cell wall - (reply: 1)
      
1754. Storage of whole cell lysates - How? (reply: 2)
      
1755. transfecting primary cells - (reply: 1)
      
1756. Why use PBS before trypsinizing cells - (reply: 4)
      
1757. Cell lysis before measuring total protein content - Which method for lysis can be combined with the Bradford method? (reply: 4)
      
1758. Question about doing RNAi in primary cells - (reply: 3)
      
1759. problem transfecting pc-3 cells - (reply: 2)
      
1760. A Procedure for Freezing and Thawing Bacterial Cells - (reply: 2)
      
1761. insert is being kicked out during cell growth - (reply: 3)
      
1762. Detecting proteins in Mouse embryonic fibroblast - Any tips on making cell lysates of MEFs? (reply: 2)
      
1763. Isolating RNA from a small number of cells - (reply: 1)
      
1764. Cell Tracing Software - Searching for a quicker way!!! (reply: 2)
      
1765. Cell growth! - Opinion needed (reply: 2)
      
1766. cells going away - (reply: 3)
      
1767. Purchasing cells - (reply: 2)
      
1768. pituitary cell lines sex - (reply: 1)
      
1769. Plasmatic cell membrane isolation (URGENT!) - (reply: 3)
      
1770. Raji cell subculture - Culturing Raji cells: DMEM or RPMI? (reply: 4)
      
1771. Arabidopsis cells suspension transformation - (reply: 3)
      
1772. How to tell if competent cells or SOC are contaminated? - (reply: 14)
      
1773. Cells won't pellet - (reply: 2)
      
1774. Transcient transfection of HaCaT cells - which is the most effective method? - (reply: 2)
      
1775. what competent cells should I use to clone big size DNA - (reply: 8)
      
1776. Dissociation of HaCaT cells without using trypsin - will PBS/EDTA work? - (reply: 4)
      
1777. does FITC enter the cell? - (reply: 1)
      
1778. Where to get Granta 536 Cells - (reply: 4)
      
1779. Cell type or Cell line? - (reply: 1)
      
1780. Moving Dots in cell culture - Contamination? - (reply: 7)
      
1781. immunostaining of Drosophila S2 cells - (reply: 1)
      
1782. Drosophila cell culture - (reply: 3)
      
1783. protein expression in mammalian cell - (reply: 1)
      
1784. Culture problems of CRL-2331 cells (ATCC) - (reply: 3)
      
1785. Can adding cold medium (4 degree) synchronize cells? - (reply: 2)
      
1786. electroporation of PC12 cells - (reply: 1)
      
1787. ES cell genomic DNA as PCR template for screening - (reply: 5)
      
1788. RNA prep (Trizol) with small cell numbers - How low can you go? (reply: 13)
      
1789. Cell detachment for flow cytometry expts - (reply: 5)
      
1790. Slow growing cells - (reply: 6)
      
1791. how the mouse ES cells should look like? - (reply: 1)
      
1792. About cell diameter - Help,please browse (reply: 1)
      
1793. All XL1Blue cells have kana resistance or what? - (reply: 1)
      
1794. Protoreaction of Protoplasm - New approaches to Cell Biology and Physiology (reply: 1)
      
1795. EDTA-free cell detachment - (reply: 4)
      
1796. Problems with Ba/F3 cell culture - (reply: 1)
      
1797. help: Drosophila cell culture - (reply: 1)
      
1798. Washing with Trypsin EDTA - (reply: 2)
      
1799. Problem growing dh5alpha cell - (reply: 2)
      
1800. CHO Cell revival - (reply: 2)
      
1801. Cells ignore my cover glass, growing around it on the plastic - What cower glass should be? (reply: 1)
      
1802. How to isolate and culture murine epidermal langerhans cells - (reply: 2)
      
1803. DH5 alpha competent cells instead of M15? - competent cells for pQE30 construct (reply: 2)
      
1804. Neural Marker For Cell Typing and Maturation - Neural Marker For Cell Typing and Maturation (reply: 4)
      
1805. Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy? (reply: 2)
      
1806. Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy (reply: 3)
      
1807. Transfection of coronary artery endothelial cells - (reply: 1)
      
1808. Stem cell transfection - (reply: 2)
      
1809. media for revival of cell lines - (reply: 1)
      
1810. cell detachment - help (reply: 3)
      
1811. transfection in eukaryotic cells - (reply: 1)
      
1812. Able C and Able K competent cels - Has anyone use these cells before (reply: 2)
      
1813. stable transfection of MDAMB231 and Malme3M cells. - (reply: 1)
      
1814. bioinformatics in cell signalling - use of bioinformatics (reply: 1)
      
1815. DNA fragmentation in apoptotic cell - (reply: 1)
      
1816. Marker for plasma cell staining - (reply: 1)
      
1817. Inactivated/activated FBS - is it critical for healthy cells (reply: 7)
      
1818. AGS Cell - Medium for AGS Cells (reply: 2)
      
1819. Adding H2O2 to cell culture - How to calculate? (reply: 4)
      
1820. how to isolate T cell and DC in the same sample? - (reply: 2)
      
1821. cell line for lentivirus titration - (reply: 1)
      
1822. Minipreps from Genehogs cell line - (reply: 1)
      
1823. ask some question about cell size of brain - help (reply: 2)
      
1824. Sick primary cells - (reply: 1)
      
1825. Role of PBS in cell culturing - (reply: 1)
      
1826. Does anyone know some specific marker of mouse hepatocytes? - (reply: 2)
      
1827. Fixing time for IF 293 T cells - (reply: 3)
      
1828. How to culture rat dorsal root ganglia cell? - (reply: 1)
      
1829. E.coli cells in the building not behaving. - (reply: 8)
      
1830. Protocal for DAPI/ Hoechst staining adhering cells - (reply: 3)
      
1831. TRANSFECTED 293 CELL FOR Luciferase assay - Dual Luciferase assay (reply: 8)
      
1832. how 2 quantify bacteria cells? - (reply: 4)
      
1833. Harvesting of cells before fractionation - escape of proteins? (reply: 3)
      
1834. Shipping cells in suspension culture - (reply: 6)
      
1835. Problems with STBL3 cells - Digesting DNA from STBL3 cells (reply: 7)
      
1836. hela cells - (reply: 12)
      
1837. endothelial cells' staining - (reply: 7)
      
1838. cre recombinase - in cell lines (reply: 2)
      
1839. effect of trypsinization on cell viability? - (reply: 3)
      
1840. siRNA transfection reagent for primary cultured cells - (reply: 2)
      
1841. Handling of cell extracts - (reply: 1)
      
1842. pcDNA 3.1/pTarget and stable cell line - (reply: 10)
      
1843. PBS causes cell death in transfection - (reply: 8)
      
1844. Cells are stuck - (reply: 4)
      
1845. Recognizing whether the cells are cancerous. - (reply: 1)
      
1846. Serum for cell culture - difference on regular vs dialyzed vs heat-inactivated FBS (reply: 3)
      
1847. Who can tell me how can I get something about L428 cell line? - (reply: 1)
      
1848. Red Blood Cells exploding ! - (reply: 2)
      
1849. loss of His tag from protein made in mammalian cells? - Anyone have experience with His tag being cleaved off? (reply: 5)
      
1850. How does the cells of the innate immunity gets rid of the pathogens? - (reply: 4)
      
1851. Generation/Transformation into DY380 cells - (reply: 4)
      
1852. Baculovirus Expression - Sonicate infected cells? (reply: 2)
      
1853. Has anyone used 8E5 cells as standard for HIV qPCR? - HIV standards (reply: 1)
      
1854. Synchronize hela cells with double thymidine block - mechanism of double thymidine block (reply: 1)
      
1855. Dendritic Cells Immunotherapy - (reply: 6)
      
1856. house-keeping primers for Cos7 cells - (reply: 4)
      
1857. DNA isolation from single cell/embryo and WGA - (reply: 1)
      
1858. RAW264.7 Cells .. anyone familiar with this cell line? - need help please (reply: 2)
      
1859. Dendritic cell novice - (reply: 3)
      
1860. adapting cells to CO2-Independent Medium - (reply: 1)
      
1861. Help me - vero-MCF-7 Hela cells culture (reply: 1)
      
1862. DNA methylation in Raji cell line - (reply: 1)
      
1863. Where my Vero cells has gone? - Mysterious dissapearing during experiment (reply: 6)
      
1864. How long does take the cell cycle in HepG2 cell line? - (reply: 1)
      
1865. how did it happen? - cells grow normally but when in SFM media, things get worse (reply: 9)
      
1866. T cell activation. - (reply: 1)
      
1867. how to keep cells in stock and how tocount the cells ! - (reply: 1)
      
1868. Alizarin red/Trypan blue stain - (reply: 3)
      
1869. genetic markers for cell lines - (reply: 1)
      
1870. coat culture dish for HEK-293T cells? - (reply: 5)
      
1871. Needing a consistant cell suspension - Non-homogenous cells make me crazy! (reply: 2)
      
1872. problems with Ghost cells - (reply: 1)
      
1873. transfecting HeLa cells for the first time... - help needed....please (reply: 4)
      
1874. bisulfite sequencing: cell lines vs tumors - (reply: 3)
      
1875. cell transfection in 96 well plate - using lipofectamine 2000 (reply: 2)
      
1876. cell type specificity ? - (reply: 1)
      
1877. Autofluorescence elimination from cell aggretates - Immunostaining troubleshoot: Autofluorescence (reply: 1)
      
1878. Passage number and behaviour of cell line - (reply: 10)
      
1879. plasmid incompatibility in mammalian cells? - (reply: 1)
      
1880. Mycoplasma Testing in culture medium - (reply: 2)
      
1881. what's the difference between 293FT and 293T - stable cell lines from 293Ft or 293T cell lines (reply: 1)
      
1882. Anti Lamp-1 or Lamp-2 for COS cells? - (reply: 3)
      
1883. Serum starvation of cells - (reply: 2)
      
1884. Slit cells or add antibiotics, which is first after stable transfection - (reply: 8)
      
1885. Transfection - Hepg2 cell lines (reply: 2)
      
1886. will the passage number affect the performance of THP-1 cells - (reply: 1)
      
1887. change in cell morphology in culture - cell culture (reply: 2)
      
1888. A drug to inactivate glial cells - (reply: 2)
      
1889. Has anyone tried to sonicate Jurkat cells for ChIP assay? - (reply: 7)
      
1890. Duration of cell line transfected - (reply: 2)
      
1891. macrophage cell line - (reply: 3)
      
1892. antibiotic dose response for suspension cell - (reply: 2)
      
1893. Suspension Culture - 293H cells clumping (reply: 2)
      
1894. Emergent: Toxicity of cell lysis buffer :( - (reply: 2)
      
1895. My competent cells are misguiding me - (reply: 3)
      
1896. Help with endothelial cells - Endothelial Cells Culture (reply: 4)
      
1897. Did anyone ever do DCF assay using M17 cell line? - For help (reply: 1)
      
1898. Cell lysis for westerns? - Immediately after harvest?? (reply: 8)
      
1899. Cell Membrane Permeability - (reply: 1)
      
1900. Quantity of genomic dna in human cells - (reply: 5)
      
1901. DRG cell culture - (reply: 6)
      
1902. siRNA Stable transfection in human fibroblast cells - (reply: 6)
      
1903. nuclear extract - protocol for cell fractioning (reply: 1)
      
1904. Cell proliferation/viability assay - use of WST and 1-Methoxy PMS (reply: 4)
      
1905. Question about stem cell attachment - (reply: 1)
      
1906. Add L-glutamine in cell culture medium? - (reply: 5)
      
1907. maximum passage number for T47D cells - (reply: 2)
      
1908. Mycoplasma contamination - detection and de-contamination (reply: 7)
      
1909. HOW to culture bone marrow monoclear cells(BMMNCs)? - (reply: 6)
      
1910. transfection of Hek 293A cells - (reply: 5)
      
1911. trypsin and chemotrypsin - does calcium protect cells from chemotrypsin (reply: 2)
      
1912. cell scraper - (reply: 4)
      
1913. How to detach adherent monocytes? - Trypsin doesn’t work (reply: 6)
      
1914. Trypsin does not work - need to detach monocytes (reply: 12)
      
1915. Software to draw a cell compartiment schematically ? - Do you know a such soft? (reply: 2)
      
1916. Transfection of cells by viral DNA - (reply: 4)
      
1917. How to detect receptor on the cell surface? - using the less viral protein possible (reply: 1)
      
1918. neural progenitor cell death in multi-well plate - (reply: 10)
      
1919. Transfecting 293 cells using oligofectamine - (reply: 2)
      
1920. Hybridoma Cell lines - How to do it (reply: 1)
      
1921. THP-1 - any information about this cell line (reply: 1)
      
1922. more contamination owes - spherical cell like contamination (reply: 8)
      
1923. proliferating cells - (reply: 6)
      
1924. C2C12 cells model for differentiation - (reply: 1)
      
1925. Cell breakage - (reply: 1)
      
1926. about glucose uptake test in hepatocytes - (reply: 2)
      
1927. Resuspending mycobacterial cells? - (reply: 2)
      
1928. Glycerol gradients - glycerol gradients for cell extract fractionation (reply: 2)
      
1929. Testing for binding of mutant proteins to cell surface receptors - (reply: 3)
      
1930. Fibroblast and epithelial cells in tumor samples and primary cultures - (reply: 2)
      
1931. Growing conditions for OE21 & OE33 cells - (reply: 1)
      
1932. DNA Repair in different cells - (reply: 2)
      
1933. Extraction of red blood cells - (reply: 1)
      
1934. Getting crazy because of cell contamination - (reply: 27)
      
1935. Cell membrane marker - (reply: 1)
      
1936. lncap cell culture - (reply: 3)
      
1937. ICC: High fluorescence cell body staining - secondary staining all neuron cell bodies (reply: 7)
      
1938. cell fractionation plus IP - (reply: 12)
      
1939. Immunohistochemistry - CD4 and CD8 cell (reply: 1)
      
1940. Lab dealing with factors affecting cell membrane permeability - (reply: 1)
      
1941. Cell culture contaminations - (reply: 6)
      
1942. passaging 293T cells - (reply: 5)
      
1943. Concentrating conditioned medium from cells - (reply: 6)
      
1944. lysosomes in cells - (reply: 2)
      
1945. help with smooth muscle cell culture - (reply: 4)
      
1946. Harvesting cells for Western - what protease inhibitors do you add to PBS? - (reply: 4)
      
1947. HELP with cell research.. - (reply: 4)
      
1948. making more chemically competent cells - (reply: 6)
      
1949. DNA stain for viable FACS cell sorting? - (reply: 7)
      
1950. Western blots on contaminated cell cultures? - Mycoplasma (reply: 1)
      
1951. Trouble with IEC-6 cell transfection - (reply: 3)
      
1952. anyone has EcR293 cells? - (reply: 5)
      
1953. Sphere cells suspension culture - (reply: 2)
      
1954. Searching specific Cell line - (reply: 1)
      
1955. Freezing cells - (reply: 2)
      
1956. Help me....About the 3T3-L1 Cells - (reply: 2)
      
1957. COS7 cells doesn't attach to the flask - (reply: 1)
      
1958. Mouse ES cell culture - ES cell culture for the purpose of gene targeting (reply: 3)
      
1959. siRNA transfetion in MEL (murine erythroleukemia) cell - (reply: 3)
      
1960. HeLa in suspension culture - I want to keep HeLa cells in suspension culture (reply: 2)
      
1961. Proetin expression in insect cells - (reply: 1)
      
1962. How many cells (nuclei) per ml pellet? - (reply: 3)
      
1963. free style 293 cells - (reply: 4)
      
1964. Glycyrrhizin (GL) and cell culture - (reply: 1)
      
1965. Transfecing N2A cells with Jet Pei - Transfecing N2A cells with Jet Pei (reply: 2)
      
1966. transfection of HiFive Cells? - Baculovirus Expression (reply: 1)
      
1967. Migration -or rather non-migration- of smooth muscle cells - (reply: 4)
      
1968. ES and MEF cell - (reply: 1)
      
1969. vector with H1 promoter be transfered to mouse cells? - (reply: 3)
      
1970. How to reduce cell clumping for immunocytostaining - (reply: 1)
      
1971. Websites or books about stem cell culture - (reply: 2)
      
1972. primary culture transfection problems - any transfection methods on primary granulosa cell (reply: 1)
      
1973. Immunofluorescence - Question about storing cells grown on slides (reply: 4)
      
1974. ATCC cell lines - How is your experience? (reply: 1)
      
1975. Extensive Cell Death with Serum Free Media - How to Avoid Extensive Cell Death with Serum Free (reply: 8)
      
1976. siRNA transfection of non-adherent lymphoma cells - (reply: 2)
      
1977. FACS control high background - more stained cells in control than in sample (reply: 4)
      
1978. need help on learning cell and tissue culturing - (reply: 6)
      
1979. PC3 cell line - Confused (reply: 8)
      
1980. can I re-freeze the 4% paraformaldehyde for longer storage? - leave cells in 4% paraformaldehyde 4 FACS? (reply: 3)
      
1981. How to spread exact amount of cells / agar plate - determine cfu (reply: 11)
      
1982. Genomic DNA purification from few Tissue Cultured cells - (reply: 2)
      
1983. Cell permeabilisation strategies - (reply: 1)
      
1984. PCR primers for Mycoplasma detection? - Publication gives many, which to choose? (reply: 1)
      
1985. Silly question: is DMSO sterile by itself, to add it to cells? - Lab stock bottle could be used? (reply: 6)
      
1986. How to grow adherent cells without attachment??? - (reply: 2)
      
1987. Cell clumping during thawing - (reply: 2)
      
1988. mouse Fibroblast specific surface markers??? - how to gate out mouse Fibroblast cells in FACS (reply: 1)
      
1989. antibiotics cell transfection - transfection (reply: 2)
      
1990. what's the best system to get siRNA into a cell? suggestions - (reply: 1)
      
1991. contamination of cell culture by another cell type - (reply: 1)
      
1992. Lysis buffer - muscle cells lysis (reply: 1)
      
1993. Measuring nitrite from LPS-activated RAW264.7 cells - (reply: 10)
      
1994. Help with defective cell lines - (reply: 1)
      
1995. Staining HT29 cells with TLR Abs and FITC, TRITC and DAPI - (reply: 6)
      
1996. How to measure neuronal cell differentiation? - (reply: 1)
      
1997. Stable transfection of S2R+ and Clone8 cells - (reply: 1)
      
1998. Cell Fusion - Fusing spleen cells with myeloma cells using PEG (reply: 1)
      
1999. How to do transfection on 293 cell - (reply: 4)
      
2000. Cell Death. optical spillovers.alternative assay - Need an assay for apoptosis (reply: 1)
      
2001. Immunocytochemistry for identifying vascular smooth muscle cells - (reply: 2)
      
2002. Fixing cells for performing immunoflourescense? - Help with protocol (reply: 3)
      
2003. Have you ever add IPTG to TOP10 cell (Invitrogen) - competent cell, IPTG (reply: 1)
      
2004. Difference between cell proliferation and cytotoxicity kits? - (reply: 2)
      
2005. Preserving RNA - How to store lised cell (reply: 3)
      
2006. Trypsin vs Non Enzymatic - (reply: 2)
      
2007. Bioruptor for sonication in mammalian cells - (reply: 2)
      
2008. transfection - selection of trransfected SH-SY5Y cells (reply: 3)
      
2009. where is the mycoplasma from?? - (reply: 9)
      
2010. Who killed my cells??? - Toxicity during transfection (reply: 3)
      
2011. Serum for aminal cell culture - understanding concepts (reply: 1)
      
2012. Transfection reagent for HT-29 and HCT-116 cells - (reply: 4)
      
2013. TSA Concentrations for Pancreatic Cell lines - (reply: 3)
      
2014. MCF-10A transfection question - what to do with slow growing cells. . . (reply: 1)
      
2015. mouse aortic smooth muscle cell isolation - cell culture (reply: 1)
      
2016. FACS - permeabilization of cells without killing (reply: 1)
      
2017. SOS call: ChIP assay in Huh7 cells - How many cells? And primer position? (reply: 3)
      
2018. DC harvesting for FACS - dendritic cells (reply: 2)
      
2019. DMSO or Ethanol - which is relatively less toxic to cells - (reply: 5)
      
2020. about cell sonication - (reply: 2)
      
2021. Huvec cell number ? - (reply: 1)
      
2022. Subculturing cells grown in serum free media - (reply: 2)
      
2023. live cell immunofluorescence - is my thoughts correct (reply: 6)
      
2024. How to screen mammalian cells clones? - (reply: 3)
      
2025. questions about mass spectrometry and cell culture - (reply: 1)
      
2026. Commonly used endothelial cells - (reply: 2)
      
2027. Antibody for inflammatory cells - (reply: 2)
      
2028. aspecific bands in cellculture protein, not in tissue - sds page (reply: 3)
      
2029. Primary Cell Culture - Tips for PHK culture (reply: 2)
      
2030. making homogeneous cell lysate suspensions - (reply: 1)
      
2031. Primary endothelial cells not attaching - (reply: 5)
      
2032. How to injure cell wall of chlorella for transformation - (reply: 2)
      
2033. HepG2 cell line - what is the doubling time for HepG2 cell line? (reply: 11)
      
2034. transfection of differentiated Caco-2 cells - (reply: 1)
      
2035. Has anybody generated stable cell lines using HepG2 cells? - Stable cell lines (reply: 5)
      
2036. Cell cycle (phase) analysis by FACS analysis - PI staining to gate different phases of cell cycle (reply: 1)
      
2037. calculating mean doubling times for adhesion cells - Mean Doubling time for adhesion cells (reply: 1)
      
2038. Mycoplasma - input needed! - Please help me by answering these questions! (reply: 6)
      
2039. Cell adhesion assays/protocols - Any suggestions? (reply: 5)
      
2040. Cell attachment - cells and jam (reply: 1)
      
2041. How to eliminate mycoplasma contamination? - Help (reply: 2)
      
2042. Immunofluorescence - cell surface protein detection - How do I detect only cell surface expressed prot. (reply: 3)
      
2043. Cell count on MCF-7 - (reply: 5)
      
2044. How to choose the flask shape and volume for Vero cells? - Is it possible to reduce? (high-cost reagents) (reply: 1)
      
2045. 5-AZA/TSA upregulation of gene in cell lines - Gene induction (reply: 3)
      
2046. cell lines and their particularities - where is the info (reply: 1)
      
2047. A quick question about sonication to lyse cells - (reply: 4)
      
2048. Separation of dead cells from cultures - (reply: 8)
      
2049. siRNA in Drosophila S2 cells? - (reply: 4)
      
2050. methods to 'quantify' morphology change in siRNA treated cells - (reply: 1)
      
2051. extract RNA from frozen cells - (reply: 6)
      
2052. How to avoid contamination of primary cells during preparation - (reply: 1)
      
2053. Function of beta-mercaptoethanol in cell culture medium - (reply: 6)
      
2054. RNAi in CHO cells - (reply: 5)
      
2055. Black Debris in T-Cells - debris in my cell culture (reply: 3)
      
2056. How do I make more competent cells? - by growing up and freezing glycerol stock? (reply: 39)
      
2057. cell cycle analysis of transiently transfected cells - (reply: 1)
      
2058. Mold Contamination in cell culture - Mold and Cell Culture (reply: 7)
      
2059. Storage of cell lysate - (reply: 7)
      
2060. competent cells question - (reply: 3)
      
2061. cancer cells on agar - growing adherent cancer cells on agar plate (reply: 2)
      
2062. Growing cells on soft agar - MCF-7 cells on agar plates (reply: 1)
      
2063. Bacterial cell wall protein refolding problem - protein refolding (reply: 4)
      
2064. What the chance to get live cells? - (reply: 11)
      
2065. Need advice on FACS for cell cycle analysis - (reply: 3)
      
2066. Transfection of 293T cells on coverslips - (reply: 1)
      
2067. Extracellular Matrix - 3t3 cells lay down ecm? (reply: 2)
      
2068. Clumping of MCF7 cells - (reply: 3)
      
2069. kato-III transfection - Help with transfecting semi-adherent Kato-III cell (reply: 1)
      
2070. Cell specific Sonication - How different are cell types (reply: 1)
      
2071. DNA Sonication of quiescent cells - (reply: 1)
      
2072. Cell counting - (reply: 2)
      
2073. dendritic cell isolation - cell culture (reply: 1)
      
2074. Why a bacterial cell restriction enzyme not cleave foreign gene - (reply: 8)
      
2075. Morphologic changes of HeLa cell after RNAi - (reply: 7)
      
2076. Sonicating cell lysate for Westerns - (reply: 2)
      
2077. MCF-7 cell line with fibroblast - (reply: 1)
      
2078. has anybody done live cell staining? - (reply: 6)
      
2079. Apoptosis and Annexin V - Protocol for cell detatchment ? (reply: 3)
      
2080. Putting 2 plasmids in 1 e. coli cell - (reply: 4)
      
2081. cell immunofluorescence - (reply: 14)
      
2082. inducible stable cell line.... - (reply: 1)
      
2083. Preparation of Cell extracts for Western Blotting? - (reply: 4)
      
2084. assays to assess cancer cell growth? - (reply: 4)
      
2085. Single cell gel electrophoresis (comet assay) - (reply: 1)
      
2086. Transforming DH5 alpha cells with pUC19 vector - Cloning (reply: 3)
      
2087. cell markers - (reply: 2)
      
2088. Insect cell sonication protocol? - Protein Purification (reply: 1)
      
2089. tumoral cells against fibroblasts - (reply: 3)
      
2090. Fixation of cell culture with Formaldehyde - need protocol (reply: 3)
      
2091. Single cell isolation from human tumor cells - human tumor cell isolation (reply: 1)
      
2092. best cell colture media for in vivo imaging - (reply: 3)
      
2093. Competent cell preparation - XL1-blue (reply: 4)
      
2094. A question about cell stain: fix the cell have fusion protein - (reply: 1)
      
2095. regulation of eukaryotic cell cycle - cell bio (reply: 1)
      
2096. How to get rid of contamination in stable transfected cells - (reply: 3)
      
2097. Differentiating Hep G2 Cells - (reply: 1)
      
2098. how can i culture virus in primary cell lines? - microbiology (reply: 1)
      
2099. Regulation of Eukaryotic cell cycle - cell bio (reply: 3)
      
2100. coatings for growing cells on glass coverslips - tissue culture and confocal microscopy (reply: 4)
      
2101. Wash cells or not after trypsinization? - (reply: 54)
      
2102. can i freeze cell lysates for western blotting? - (reply: 3)
      
2103. question about cell thawing - help (reply: 3)
      
2104. real-time RT-PCR one cell - (reply: 2)
      
2105. Mouse splenocyte cell sizes - Cell size range (reply: 3)
      
2106. Subculturing astrocyte from E15 days mouse embryo - Technique (reply: 2)
      
2107. Screening stably transfected cells? so little? - (reply: 4)
      
2108. Lipof/Opti transfection cells died in DMEM/F12 - Transfection (reply: 4)
      
2109. confocal microscopy, fluoro beads, 3 types of cells on one slide - (reply: 1)
      
2110. Why add phenol red in trypsin? - (reply: 14)
      
2111. how to get to know cell lines? - (reply: 3)
      
2112. Cell cycle question - CKIs - (reply: 2)
      
2113. Information about cell lines - (reply: 3)
      
2114. Tansfection in drosophila S2 cells with pEGFP-N1 - pEGFP-N1 vector @ drosophila S2 cells (reply: 1)
      
2115. Is TE buffer toxic to cultured cells? - cell culture (reply: 4)
      
2116. Definition of cell confluency - (reply: 6)
      
2117. in- gel trypsin digestion - (reply: 1)
      
2118. How to seed adhering cells (HepG2) evenly in 24-well plates? - (reply: 5)
      
2119. Troubles with labeling epithelial cells with CFDA-SE - Troubles with labeling epithelial cells with CFDA- (reply: 1)
      
2120. Incubation - Effect of other cell lines during incubation (reply: 1)
      
2121. hybridoma cell question - (reply: 4)
      
2122. growing epithelial cells from tumor - (reply: 2)
      
2123. differentiate THP-1 cells - differentiate THP-1 cells (reply: 4)
      
2124. In Vivo cell proliferation - (reply: 3)
      
2125. How to sort real GFP positive cells? - autofluorescence VS GFP (reply: 2)
      
2126. detecting Mycoplasma in cultures - (reply: 7)
      
2127. Does siRNA transfection dependents on cell type - (reply: 2)
      
2128. Do you reuse cell culture vessels? - (reply: 3)
      
2129. Stable cell line - G418 - (reply: 5)
      
2130. more help: cell culture problem - (reply: 1)
      
2131. Anybody has INS-1E cell? - (reply: 1)
      
2132. Isolation of mouse hepatocytes using Percoll - Protocol for isolation of mouse hepatocytes (reply: 3)
      
2133. cell cycle of NIH3T3 - cell cycle of NIH3T3 (reply: 1)
      
2134. get metaphase cell by nocodazole? - (reply: 1)
      
2135. Osmolarity of the mammalian cell medium - (reply: 2)
      
2136. Passage-hepatic stem cells - (reply: 1)
      
2137. Transferring cells from a 96 to 24 well plate - (reply: 1)
      
2138. Establishment of primary cell lines - (reply: 1)
      
2139. Cell counting - (reply: 3)
      
2140. Fungal Infection in mesangial cells - Primary Cell Culture (reply: 2)
      
2141. RCF safe for mammalian cell - (reply: 1)
      
2142. Freezing medium and cell counting - (reply: 6)
      
2143. Incubate suspension cells? - (reply: 1)
      
2144. Tumor cells immortal? - (reply: 5)
      
2145. Sludge when growing insect cells - (reply: 1)
      
2146. "Cell culture discard in chloros (10,000) ppm" ? - (reply: 4)
      
2147. Trypan blue cell counting - (reply: 7)
      
2148. cells transfection - transfecting DNA amounts (reply: 5)
      
2149. S2 cell expression - Protein expression (reply: 2)
      
2150. human rTGFß for mouse cell culture applications? - (reply: 1)
      
2151. How do you differentiate Hep G2 cells? - Hep G2 cells (reply: 6)
      
2152. Gene expression in mammalian cells - (reply: 6)
      
2153. How to make a stable cell line using suspension cells - (reply: 5)
      
2154. Thawing of competent bacterial cells - (reply: 2)
      
2155. Frozen transformed bacterial cells problems - (reply: 6)
      
2156. Origins of primate, canine, vertebrate cells, etc. - (reply: 1)
      
2157. does fungi swim or move? - cell culture contamination (reply: 8)
      
2158. Tissue culture and cell culture? - (reply: 4)
      
2159. Cell culture contamination - small black adherent dots!! (reply: 4)
      
2160. Do all 293 cells contain an adenovirus? - Do all 293 cells contain an adenovirus? (reply: 3)
      
2161. passage number in mammalian cells - (reply: 9)
      
2162. seeding of cells for transfection - (reply: 4)
      
2163. freezing of cells - (reply: 9)
      
2164. Liver Perfusion - Hamsters Liver Perfusion and Hepatocytes isolation (reply: 2)
      
2165. Protein degradation - Recombinant protein degradation in insect cells (reply: 1)
      
2166. how to prevent HEK293 cells from coming off the plate - (reply: 4)
      
2167. How can I infect BT549 cells ? - (reply: 1)
      
2168. transfection problem---SOS! - why the cells should be split before transfection? (reply: 7)
      
2169. Buffer for red blood cell lysis for RNA extraction from blood - RNA methodology (reply: 6)
      
2170. Plant cell wall protein - remove the polysaccharide and purify the protein (reply: 1)
      
2171. Best transfection method/reagent for HeLa cells? - (reply: 4)
      
2172. MDA-MB-231 cell culture - (reply: 7)
      
2173. Problem in culturing MCF-7 cell line! - (reply: 16)
      
2174. Preparation of competent cells of pichia - (reply: 1)
      
2175. Why use DMSO and FCS for freezing cells? - (reply: 5)
      
2176. Problem in culturing PZ-HPV-7 cell line - (reply: 4)
      
2177. Lentiviral vector for infecting primary cells? - Any suggestion for a suitable vector? (reply: 1)
      
2178. Murine markers on hematopoietic stem cells (CD34, Sca-1, etc) - Looking to identify markers on megakaryocytic progenitors (reply: 2)
      
2179. Filtration of primary cell culture, how?? - (reply: 2)
      
2180. breast primary culture cells - (reply: 2)
      
2181. The best way to get nuclear proteins from mammalian cells - How to break your cells? (reply: 1)
      
2182. filtering for cell culture stuff - filter cell culture (reply: 5)
      
2183. transfection of Caco2 cells - (reply: 1)
      
2184. transfection of single copy of plasmid per cell - (reply: 3)
      
2185. Natural Killer cell - Natural Killer cell (reply: 1)
      
2186. looking for cell line: CEMX174(T1) - (reply: 2)
      
2187. tgf-beta in cell culture - (reply: 1)
      
2188. SF9 cells - (reply: 2)
      
2189. How to attach suspension cell to coverslips? - (reply: 2)
      
2190. Natural Killer cells - (reply: 1)
      
2191. How to culture T cells - (reply: 1)
      
2192. Stable Transfection in Sf9 cells - Stable Transfection in Sf9 cells (reply: 5)
      
2193. How to handle and transfect suspension cells? - (reply: 7)
      
2194. B-gal staining of cells - (reply: 1)
      
2195. stable transfection of mouse embryonic stem cells - (reply: 2)
      
2196. Need HeLa cell extract prepartion protocol - wait your answer online (reply: 1)
      
2197. question about CD5 - CD5+ cells (reply: 1)
      
2198. bands from tissue one size, from cells - size doubles - help! (reply: 4)
      
2199. How to transfect siRNA into suspension cell lines? - (reply: 3)
      
2200. Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
      
2201. Transfection of CaCo2 Cell lines - (reply: 2)
      
2202. red blood cell lysis - (reply: 6)
      
2203. Freezing HeK'n cells - (reply: 2)
      
2204. RNAi in Jurkat cells - (reply: 3)
      
2205. Cell fusion protocol to generate heterokaryons - (reply: 4)
      
2206. Transfection - Cell death after transfection - (reply: 6)
      
2207. hepatocytes derived stem cell - hepatocytes derived stem cell (reply: 3)
      
2208. FACS analysers - Purchase of a FACS analyser and cell sorter (reply: 3)
      
2209. FACS analysis in adherent cell lines - FACS analysis in adherent cell lines (reply: 3)
      
2210. Stable cell lines not overexpressing my gene of interest! - Technical problems (reply: 2)
      
2211. The function of EDTA for harvest cells - (reply: 2)
      
2212. co-culture of two adherent cells - (reply: 4)
      
2213. Cos 1 Cells - who supples cos 1 cells (reply: 2)
      
2214. Cell lysis when isolating dendritic cells - (reply: 1)
      
2215. breast cancer cell primary culture - (reply: 2)
      
2216. hep-2 & Hep-G2 cell line - difference between hep-2 & Hep-G2 cell line (reply: 22)
      
2217. chemical synthesized siRNA and rapidly dividing cells - (reply: 2)
      
2218. Cell contamination, needs the help urgently - How to remove? (reply: 2)
      
2219. Cell counts using absorbance/turbidity - Cell counting (reply: 1)
      
2220. EDTA only for cell dissociation - (reply: 7)
      
2221. Bringing tumor line from animal back to cell culture - (reply: 2)
      
2222. where to buy sterile grease used for isolate cell clone? - (reply: 2)
      
2223. Sterilizing a Cell Culture Room - We had a flood + mold, where do I start? (reply: 1)
      
2224. please recommend me a RNA isolation kit - RNA isolation from 100 tumor cells (reply: 1)
      
2225. the cancer cell growth - Problem with the cancer cell growth (reply: 3)
      
2226. Anybody has ever transfected EL4 cells with siRNA? - help! (reply: 3)
      
2227. Small competent cells - (reply: 3)
      
2228. RT-PCR of different cell lines - (reply: 1)
      
2229. Please answer a layman's questions - Very basiscs of Cell and Genetics (reply: 3)
      
2230. cancer cell culture - protocols on cancer cell culture (reply: 8)
      
2231. aml-2 cells - contamination in aml-2 cells (reply: 1)
      
2232. Mammalian cell line - monolayer - monolayer growth of mammalian cell line (reply: 1)
      
2233. stem cell isolation - stem cell markers and stem cell sphere (reply: 3)
      
2234. How many cell passages to be safe ? - (reply: 8)
      
2235. Cell fixation - (reply: 6)
      
2236. Contamination issue in cell culture - (reply: 109)
      
2237. Freezing down cell lysate - (reply: 2)
      
2238. Visualizing Cell Pellet - (reply: 1)
      
2239. O2 vs H2O2 - does H2O2 decease the O2 content in cell medium (reply: 1)
      
2240. LCL B cell culture - Death within 24 hours of thawing - LCL b cells are dying within 24 hours of thawing. (reply: 3)
      
2241. Problems with B-cell line BJAB - clumps formed by cells in culture (reply: 1)
      
2242. Stable transfection of S2 cells - (reply: 1)
      
2243. Cell exposured to UV light for one hour - is the cell OK? (reply: 1)
      
2244. ATDC5 cell line - i am looking for this cell line (reply: 2)
      
2245. co transfection for preparing stable cell - (reply: 1)
      
2246. Dendritic cell -isolation ---viabillity is very low - Immunology (reply: 3)
      
2247. Cell lysis for peptide binding assay - (reply: 3)
      
2248. measuring apoptosis after treatment of cells with ZVAD-FMK - (reply: 1)
      
2249. cell fixation - How to fix cells? (reply: 10)
      
2250. Suspension Culture - Can it be transformed into adherent cells? (reply: 5)
      
2251. what types of cells is a mammalian cell? - (reply: 3)
      
2252. Help! Cell staining problem - (reply: 3)
      
2253. Help with stable cell lines - (reply: 3)
      
2254. choosing insect cell for high-cystine protein expression - (reply: 1)
      
2255. cell titer glo - relative light unit (reply: 1)
      
2256. Cells in collagen gel - Growing mammalian cells in collagen gel (reply: 1)
      
2257. non-adherent cells immunostaining - lymphocytes immunocytochemistry (reply: 3)
      
2258. H295R Cell transfections - (reply: 2)
      
2259. Equipment requirements to culture cells - information needed on kidney cells (reply: 2)
      
2260. cells in the earth - (reply: 4)
      
2261. How little competent cell do you use for transformation - (reply: 2)
      
2262. Trypsin free detachment?? - (reply: 2)
      
2263. About R28 cells culture - (reply: 2)
      
2264. Freezing and thawing of cells - (reply: 112)
      
2265. transfection of thp1 cells ?? - (reply: 3)
      
2266. Human Macrophage cell line - quick help please - (reply: 3)
      
2267. MDA-MB231 cells and problems - (reply: 1)
      
2268. cell count & viability - does DMSO affect trypanblue staining after thawing (reply: 6)
      
2269. proliferation assay - labeling cells with 3H thymidine (reply: 2)
      
2270. Primary epithelial cell line - Isolation of epithelial cells (reply: 3)
      
2271. Selection of clones during transfection - Concentration of G418 to be added to the cell. (reply: 7)
      
2272. CaPO4 transfection prevents trypsin activity - Trying to split after transfecting (reply: 1)
      
2273. Cell Biology - protocol for reactive oxgyen species (reply: 1)
      
2274. single cell PCR - (reply: 8)
      
2275. Plant cell preparation - Flow cytometry (reply: 1)
      
2276. Transfection using PEI - Transfection of 16HBE and CFBE cells (reply: 4)
      
2277. poly(A)-RNA extraction from cells - (reply: 1)
      
2278. Tet-responsive cell line - cells cannot become confluent (reply: 1)
      
2279. Transcriptional activity in Stable cell line. - (reply: 3)
      
2280. 293T cells go wild after >50 passages??? - protein expression changed!?? (reply: 1)
      
2281. A question about adenovirus infection 293 cells - (reply: 5)
      
2282. What are the cells comprising PBMC?? - (reply: 1)
      
2283. Methods for mechanical disruption of cell - (reply: 3)
      
2284. MC57G cells and transfection - (reply: 1)
      
2285. Cell disruption without sonication - (reply: 4)
      
2286. transfection efficiency - mammalian cell transfections (reply: 1)
      
2287. mycoplasma and fungus - tissue culture infection (reply: 2)
      
2288. pBABE Comp. Cells - Special cells for transformation (reply: 3)
      
2289. preventing infection of cell cultures - (reply: 3)
      
2290. Any ideals for cell fragmentation? - (reply: 2)
      
2291. problems with cell stimulation - (reply: 2)
      
2292. cell lysis - How to lysis cells (reply: 2)
      
2293. in-competent cells - (reply: 1)
      
2294. DMSO and cell cycle - (reply: 2)
      
2295. Cell Cycle/DNA/RNA Unanswered Questions - test questions involving genetic info (reply: 2)
      
2296. cell culture RNA extraction - cell culture RNA extraction (reply: 4)
      
2297. cell Transfection and western blot - transfection and western (reply: 2)
      
2298. Origami B cells grow too slowly!! - (reply: 1)
      
2299. Where to buy cells? - Where to buy LnCaP cells? (reply: 1)
      
2300. about LNCap cell lines - (reply: 1)
      
2301. CBP expression problem in mammalian cells - (reply: 2)
      
2302. CBP expression problems in mammalian cells - (reply: 1)
      
2303. help: primary endothelial cell culture! - (reply: 3)
      
2304. problem by using percoll to purify primary cell - (reply: 1)
      
2305. Cell Biology - tumor growth (reply: 2)
      
2306. the host cell of plasmid:pBmIEGLacZ-BmIEGhyg - (reply: 1)
      
2307. About Cell Cycle - (reply: 1)
      
2308. T cell proliferation - problems (reply: 1)
      
2309. Help: How can I make cell line from MEF cells? - (reply: 2)
      
2310. lymphocytes' RNA extraction - How many cells (reply: 1)
      
2311. cell adhesion - (reply: 1)
      
2312. Selection of cell ine - (reply: 2)
      
2313. 4 layers instead of a monolayer in cell culture - (reply: 2)
      
2314. Cell culture problem - weird stuff grow in my cells - (reply: 6)
      
2315. Cell staining - (reply: 1)
      
2316. Cell counting of Strongly adherent cells - (reply: 4)
      
2317. How to keep the shape of transfected Hela cell? - Cell transfection (reply: 1)
      
2318. How to detect drug's cytotoxcity by MTS - (reply: 2)
      
2319. Cell viability assays fortissues - (reply: 2)
      
2320. How to assay the amount ofcollagen on cell/scaffold composites? - (reply: 1)
      
2321. Separate mammal cell - (reply: 1)
      
2322. PBMC-B cell - (reply: 1)
      
2323. BY-2 cells - (reply: 2)
      
2324. Plant Culture Cell - (reply: 1)
      
2325. HEK293 cell culture protocol - (reply: 1)
      
2326. stable cell line - stable cell line (reply: 1)
      
2327. cell biology - (reply: 1)
      
2328. Cell line for glutamate receptors - Glutamate receptor cell lines (reply: 1)
      
2329. KB 3-1 cell morphology - (reply: 2)
      
2330. lipofectin mediated transfectionof HepG2 cells - (reply: 1)
      
2331. Serum in Cell Culture - Use of FBS etc. in cell culture (reply: 3)
      
2332. stable transfection of epithelial cells - stable transfection in mammary breast cell lines (reply: 8)
      
2333. Purification of a cell membrane protein - (reply: 1)
      
2334. Shipping Cell Cultures - Shipping Cell Cultures (reply: 3)
      
2335. How to get enough cell alivefrom colon cancer tissue? - (reply: 2)
      
2336. using culture sup of one cell line  to induce 2nd cell line - (reply: 1)
      
2337. CHO cell clumps in bioreactor - (reply: 6)
      
2338. Stable Trasfection into Hek 293and Hela cells - (reply: 1)
      
2339. Rat Osteoblast Primary Cell Line - fetal rat calvaria (reply: 1)
      
2340. Isolation of nuclei from mammalian cells - (reply: 1)
      
2341. how to culture a cell line of "fibroblast" - (reply: 5)
      
2342. Re: how to seperate deadsuspension cells from alive ones - (reply: 1)
      
2343. Cell Line Establishment - (reply: 1)
      
2344. mutation in cancer cell line - (reply: 1)
      
2345. Subculturing primary Keratinocytes - (reply: 2)
      
2346. Reviving Cells from Liquid Nitrogen - (reply: 2)
      
2347. Collagen gels - Problems with growth of endothelial cells on collagen gels (reply: 1)
      
2348. cell lines for anti-inflammatory study - (reply: 1)
      
2349. how to seperate dead suspension cells from alive - (reply: 2)
      
2350. How to tell different cellstypes apart - (reply: 2)
      
2351. what solution to use for cell lysis - (reply: 5)
      
2352. Yeast in Cell Culture... - How to erase the Yeast? (reply: 2)
      
2353. Isolation of cell membrane - (reply: 5)
      
2354. Centrifuge cell at room temperature? - (reply: 1)
      
2355. Lysis of CHO and HEK Mammalian Cells for SDS-PAGE - (reply: 4)
      
2356. cells washed away - cell culture problems (reply: 6)
      
2357. Chondrocyte / synovial cellculture - (reply: 1)
      
2358. cell viability/toxicity - (reply: 3)
      
2359. Stable Transfection of large DNAinto 293 cells - (reply: 1)
      
2360. Stable Transfection of CEM cells - (reply: 1)
      
2361. Endothelial cells culture - Protocols for the culture of endothelial cells, special tips (reply: 2)
      
2362. seperation of epithelial cells and fibroblast from a biopsie - a culture of epithelial cells is needed without fibroblasts (reply: 1)
      
2363. BMP4 expressing cells - (reply: 1)
      
2364. total protein assay in cell extracts - (reply: 3)
      
2365. cell biology - lysate preparation (reply: 1)
      
2366. Labeling cells with AT32P - cell biology (reply: 1)
      
2367. Formalin fixation&paraffin-embedding of tissue culture cells - Formalin fixation&paraffin-embedding of tissue culture cells (reply: 1)
      
2368. how the seperate dead cells from living ones. - (reply: 5)
      
2369. Cell wall stain - (reply: 1)
      
2370. animal cell culture - (reply: 2)
      
2371. Chicken  ES cell - (reply: 1)
      
2372. distilled water shock of red blood cells - (reply: 4)
      
2373. Kit for Mycoplasma detection by PCR - (reply: 4)
      
2374. please help me - how to extract cd3 protein from jurkat cell (reply: 1)
      
2375. PROTEIN ASSAY - MEASUREMENT OF PROTEIN IN CELL LYSATES (reply: 2)
      
2376. Influenza inoculation of Hek293 cells - (reply: 1)
      
2377. How to extract hydrophobic proteins from yeast wall cells? - (reply: 1)
      
2378. Quantifyng Mycobacterium Cells - (reply: 5)
      
2379. Cell Lysate ELISA - Find protocol for a cell-lysate ELISA (reply: 2)
      
2380. Growth conditions for T cellline - (reply: 2)
      
2381. Isolation of cytokine-secreting cells - (reply: 2)
      
2382. Mouse B cell Activation - (reply: 1)
      
2383. memory cells - (reply: 4)
      
2384. Cell isolation form cord blood - (reply: 1)
      
2385. B cell proliferation assays - (reply: 1)
      
2386. Is there any simple  way totell a cell coming from cell division - (reply: 1)
      
2387. Retroviral infection of primaryCD4+ T helper cells - (reply: 1)
      
2388. T cell isolation from rat wholeblood - (reply: 2)
      
2389. Dendritic cell isolation - (reply: 4)
      
2390. Pituitary cell culture - (reply: 1)
      
2391. which is more stable ineukaryotic  cell,ssRNA or ds RNA - (reply: 1)
      
2392. Whole Cell PCR from BacteriaProtocols? - (reply: 4)
      
2393. single cell DNA isolation frommammalian cells - (reply: 4)
      
2394. collagen measurement andisolation in cell based - (reply: 1)
      
2395. detection of calpain activity inthe Red blood cell and other tissues - (reply: 1)
      
2396. genomic and fragmented DNAextraction from human  cells.  Ple - (reply: 1)
      
2397. How to transform the cells witht wo different plasmids - (reply: 3)
      
2398. Units of trypsin? - (reply: 1)
      
2399. PCR with cell source - (reply: 1)
      
2400. RNA isolation from a single cell - (reply: 1)
      
2401. How to prepare cDNA library fromsingle cells - (reply: 1)
      
2402. Competent Bascillus Cells - (reply: 1)
      
2403. Co-transfection Question - Will co-transfected vectors enter the same cell? (reply: 2)
      
2404. cell lysis - How do I decide what solution to use? (reply: 2)
      
2405. membrane protein purification - how to pufify cd3 protein from jurkat cell (reply: 1)
      
2406. adenovirus infection (293 cells)protocol - (reply: 1)
      
2407. white blood cells - (reply: 1)
      
2408. cell line with deletion in mitochondrial DNA - Molecular Biology (reply: 1)
      
2409. Single-cell RT-PCR with only ~10-100 Cells - (reply: 2)
      
2410. Cell senescence markers - (reply: 1)
      

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