SMP30 silencing in vivo - stem cell biology (Sep/24/2008 )

I am working on a transplantation protocol in kidney and I want to silence smp30 gene of the host animal.

Is there a way to do it? Perhaps shRNA or something else?

I didn t find any commercial product targeting smp30 in vivo

thank you in advance

Vivo-Morpholinos are a good technique for kidney. When splice-modifying Vivo-Morpholinos were injected into mice at 12.5 mg/kg once per day for four days and then sacrificed on day 5 for RT-PCR analysis, over 90% of the targeted transcripts in kidney had underwent splice modification. See:

Li YF, Morcos PA. Design and Synthesis of Dendritic Molecular Transporter that Achieves Efficient in Vivo Delivery of Morpholino Antisense Oligo. Bioconjug Chem. 2008 Jul;19(7):1464-70. Epub 2008 Jun 20.
http://www.ncbi.nlm.nih.gov/pubmed/18564870

Morpholinos without delivery moieties have been used successfully in explanted organotypic kidney cultures.

Nikopoulos G, Adams T, Adams D, Oxburgh L, Prudovsky I, Verdi J. The use of Endo-Porter to deliver morpholinos in kidney organ culture. Biotechniques. 2008 Apr;44(4):547-9.
http://www.ncbi.nlm.nih.gov/pubmed/18476819

The oligos have far less off-target RNA interactions than RNAi-based techniques such as siRNA or shRNA.

Mende M, Christophorou NA, Streit A. Specific and effective gene knock-down in early chick embryos using morpholinos but not pRFPRNAi vectors. Mech Dev. 2008 Sep 3. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/18801428

Morpholinos can target translation, splicing or miRNA maturation. The uncharged Morpholino oligo backbone does not trigger innate immune responses. They are commercially available with about two week turnaround for a custom synthesis from ordering to delivery. Targeting assistance is included.

ok thx. i am gonna check it out.