yeast contamination in my cell culture - (Jul/06/2006 )

hello everybody
i have a problem, yesterday i checked my cell cultures , some of them had yeast infection , i grow 2 different types of cells
so i discard the infected dishes and do passage for the others
today i found aagain one dish has yeast and the others no???
what may be the cause?
i checked the media , its ok and i changed the laminar hood yesterday , do cleaning as much as i could.......
the cell culture that got infected has G814 on it.

thankx

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Check the culture dish that has no infection again tomorrow.

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thankx minnie
i will do.
but i cleaned the co2 incubator with alcohol 70% and all my pippetts too, autoclaved the garbedge.....
is it likely that yeast can infect the others cultures in the co2 incubator?

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Yes

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hi spanishflower ! yeasts are present as commensals along with other organisms on our body parts. most common source of yeast contamination is hair, nails etc. so be careful while subculturing or handling your cultures.
cheers

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I agree with Shiva, yeast contamination 99% of the time comes from the scientist/technician doing the cell culture.

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Especially when the incubator is over-filled with plates.
then experimentators move the plates often, and when it's without gloves..
I know a lab that experience a lot of yeast incubation, because one experimentator make is own bread at home, and do not wear gloves while touching plate cultures.

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i believe I also have yeast contamination, but the weird thing is that they only grow in the presence of my cells, I tried to culture them in pure media and didnt grow, but when my cells re present (just fibroblasts, others not) they grow and attach to the membrane apparently.
hate them!

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I am also suffering from yeast contamination. The important thing is to know how to get rid of contamination ASAP. I have cleaned cell culture lab, incubator, water bath. I also ordered new medium and FCS and antibiotics. But the contamination happened again.... So does this mean my cell source is contaminated? I wouldn't think so as I thawed the same batch before and they growed find. So where is the problem? Could anyone help me?

Thanks

xiao29

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may be your aseptic technique...

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