Fungal Infection in mesangial cells - Primary Cell Culture (Mar/29/2005 )

Can anyone please suggest a remedy to remove fungal infections from my mesangial cells?
The mixed cultures appear fine up to about the 2-month stage and then become infected with fungi just as the mesangial cells start to grow out. The kidneys which they were harvested from were obtained under non-sterile conditions but why does any latent infection wait until this time-point to appear?

I am currently growing them in media with 2% Pen/Strep and 2% Fungazole refreshed every 3 days.

Any suggestions gratefully received!

Kelly

Could be just a very slow growing fungi, perhaps as cells start to 'grow out' that there are a lot more growth factors secreted into the media facilitating better growth.
We use nystatin to remove fungi/yeast from contaminated cultures and it works quite well. Can damage some cell line so would have to test this first.

Many thanks!!
I've tried just about everything else and had come up with the same conclusions as you. Will see if the Nystatin works out.
K