Search transfection reagent for BJ cells - (Dec/10/2007 )
Hi, everybody, we are tring to search a efficient tansfection reagent for BJ cells(ATCC number CRL-2522). Cause we have used Genejuice and Fugene 6 reagent to transfect plasmid to BJ cells. While the result was not satisfied. Do you know some more efficient transfection reagent for BJ cells or other fibroblast cells ? Tanks!
-IPS-
A small suggestion:
you can easily get free samples of transfection reagents to try from different companies. Just call your sales reps.
Also, I think Invitrogen had comed up with a new transfection reageant not so long ago: Lipofectamine LTX. You should be able to get a free sample of that easily.
Finally, have you tried reverse-transfection? That usually yields much better results for me than the normal protocol.
-chicho-
QUOTE (chicho @ Dec 13 2007, 02:32 PM)
A small suggestion:
you can easily get free samples of transfection reagents to try from different companies. Just call your sales reps.
Also, I think Invitrogen had comed up with a new transfection reageant not so long ago: Lipofectamine LTX. You should be able to get a free sample of that easily.
Finally, have you tried reverse-transfection? That usually yields much better results for me than the normal protocol.
you can easily get free samples of transfection reagents to try from different companies. Just call your sales reps.
Also, I think Invitrogen had comed up with a new transfection reageant not so long ago: Lipofectamine LTX. You should be able to get a free sample of that easily.
Finally, have you tried reverse-transfection? That usually yields much better results for me than the normal protocol.
Thanks a lot.
-IPS-