b-mercaptoethanol and stem cells - (Jul/01/2008 )

quick question:

why in ESCs media there is b-mercaptoethanol?

thanks

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To prevent differentiation, I think.

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but for that there is LIF...

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That is true. But b-m at cell culture dose creates a mild reducing environment, which somehow prevents enzyme, hormone, cytokine degradation.

Well, I just found this wonderful page, why don't have a look?
http://www.protocol-online.org/biology-for...posts/7593.html
..

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