Sphere cells suspension culture - (Sep/13/2005 )
I have some sphere cells in neurobasal medium.After 4 days the cells starts clumps together and form bigger spheres.
But i dont know why they are forming sphere cells flotting on the medium.
small spheres again attaches and form bigger spheres.
why it is so?
-seenakv-
Neurospheres will form and floating around in neurobasal medium (+N2, B27) because it's serum-free. If you add some serum they will attach.
-joy_maf-
I would like to know why theses are floatting?
what couse it to form spheres?
-Helia-