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Sphere cells suspension culture - (Sep/13/2005 )

I have some sphere cells in neurobasal medium.After 4 days the cells starts clumps together and form bigger spheres.
But i dont know why they are forming sphere cells flotting on the medium.
small spheres again attaches and form bigger spheres.
why it is so?

-seenakv-

Neurospheres will form and floating around in neurobasal medium (+N2, B27) because it's serum-free. If you add some serum they will attach.

-joy_maf-

I would like to know why theses are floatting?
what couse it to form spheres?

-Helia-