Adult stem cell differentiation - (Apr/05/2006 )
I suspect my adult stem cells are differentiating. Several cells look hypervacuolized and I'm afraid they are differentiating into adipocytes. Is that possible? Hypervacuolization can also be a sign of cells that are dying. How can I figure out the problem? Claudia
-CSCs-
QUOTE (CSCs @ Apr 6 2006, 05:04 AM)
I suspect my adult stem cells are differentiating. Several cells look hypervacuolized and I'm afraid they are differentiating into adipocytes. Is that possible? Hypervacuolization can also be a sign of cells that are dying. How can I figure out the problem? Claudia
i think u can do the Oil Red O staining to see if there was lipid droplet formed.or u can observe cells under microscope,lipid droplet is shining,the adipocytes are round and biger than your cells.
-hbmssc-
You can check by staining with protein specific for differentiated lineage. Apoptosis can be checked by AnnexinV or DNA laddering assay.
-Calvin*-