Centrifugation speed for adherent cell line - (Jan/14/2008 )

I am culturing mouse skin melanoma adherent cells. What is the suitable speed of centrifugation to settle down the cells? Can I use up to 1500 rpm for 5 min?
The pellet I get is dark color, not whitish pellet....is it due to this cell line is a highly pigmented cells that contain melanin pigment?

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What happens if you use less rpm?
I would recommend you to centrifuge as slow as possible.

We use up to 430xg

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For most cells, we use between 100x g to 500xg for 5 min. This is sufficient to get them down.

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I use 1000rpm for 5 min, then when I slowly pour out the supernatant , the cell pellet is like not settle down completely..can see some still floating. I use 15 ml Falcon tubes for centrifugation.

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100xg - 500xg is how many in rpm?

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I see on this topic some persons need to learn how a centrifuge works and what is the difference between rpm and g !!

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I know there is a formula to convert g to rpm:

RCF (g) = 0.0000118 x r x rpm^2

r = radius of rotor in cm

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Then why don't you give the g-force? That's the only relevant point as we don't know what centrifuge you have.

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I need to check back what is the rotor radius of the centrifuge in my lab. I forgot how much is it. I think should be stated in the manual book. Anyway..Thanks for ur reply.

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you can simply take a ruler and measure the radius.

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