HOW TO MAKE A STABLE TRANSFECTED CELL LINE with COS-7? - (Jun/05/2008 )
Dear all.. Hi! Im a new member in here.. nice to know about this forum..
In our lab, we deal a lot with Nuclear Receptor. We use COS-7 cell line as the host for nuclear receptor assay. The mechanism that we usually use to transfect the cell is by using lipoctamine and plus reagent method. However, since we have a quite lot of nuclear receptor plasmids, its kinda time and reagent wasting to run all assays. We have to do transfection again and again everytime we want to run an assay.
In one paper, i have seen about stable transfected HEK cell line. I just wonder, is it possible to make a stable transfected COS-7 line also? Is there any this forum member who has this kind of experiment before? If it is possible, could you please share me how to do that?
Thank you very much for the comment. I really appreciate it.. 
In our lab, we deal a lot with Nuclear Receptor. We use COS-7 cell line as the host for nuclear receptor assay. The mechanism that we usually use to transfect the cell is by using lipoctamine and plus reagent method. However, since we have a quite lot of nuclear receptor plasmids, its kinda time and reagent wasting to run all assays. We have to do transfection again and again everytime we want to run an assay.
In one paper, i have seen about stable transfected HEK cell line. I just wonder, is it possible to make a stable transfected COS-7 line also? Is there any this forum member who has this kind of experiment before? If it is possible, could you please share me how to do that?
Thank you very much for the comment. I really appreciate it..
You can make stable cell line out of any cells.
http://search.vadlo.com/b/q?sn=158621799&a...+cell&rel=0
Here are some Cos-7 specific:
http://search.vadlo.com/b/q?sn=158621799&a...table&rel=0
COS-7 cells are not difficult for stable transfection. Invitrogen Lipofactamine 2000 reagent is enough to make stable cell lines. However, plasmids from different vendors are very different. Choosing right plasmids is important for making good stable cell lines.
In our lab, we deal a lot with Nuclear Receptor. We use COS-7 cell line as the host for nuclear receptor assay. The mechanism that we usually use to transfect the cell is by using lipoctamine and plus reagent method. However, since we have a quite lot of nuclear receptor plasmids, its kinda time and reagent wasting to run all assays. We have to do transfection again and again everytime we want to run an assay.
In one paper, i have seen about stable transfected HEK cell line. I just wonder, is it possible to make a stable transfected COS-7 line also? Is there any this forum member who has this kind of experiment before? If it is possible, could you please share me how to do that?
Thank you very much for the comment. I really appreciate it..
You can make stable cell line out of any cells.
http://search.vadlo.com/b/q?sn=158621799&a...+cell&rel=0
Here are some Cos-7 specific:
http://search.vadlo.com/b/q?sn=158621799&a...table&rel=0