Creating Cell suspension - (Aug/11/2007 )
hi all,
i was just wondering if it is possible to create cell suspension out of heart and kidney for affinity chromatography? If it is possible how do u go abt doing it any recommanded protocols to adhere? i have not done this before , it wld be a great help if anyone cld guide me on this...
thanks in advance...
-Rita001-
QUOTE (Rita001 @ Aug 12 2007, 05:28 AM)
hi all,
i was just wondering if it is possible to create cell suspension out of heart and kidney for affinity chromatography? If it is possible how do u go abt doing it any recommanded protocols to adhere? i have not done this before
, it wld be a great help if anyone cld guide me on this...
thanks in advance...
i was just wondering if it is possible to create cell suspension out of heart and kidney for affinity chromatography? If it is possible how do u go abt doing it any recommanded protocols to adhere? i have not done this before

thanks in advance...

use tissues better than whole organs; try to use cytometry
-The Bearer-