How to infect T cells? Lentiviral or retroviral? - (Nov/17/2007 )
Hi, everyone, I have to infect naive t cells with Gene X.
I get two choices: lentiviral or retroviral. Which one is better?
BTW, I am trying to generate a fusion protein of Gene X--GFP or RFP.
After infection, I 'd like to analyze these cells using FACS and hope to gate out GFP or RFP+ T cells and then see other cell markers in the gated cell population.
If another fluorescence of antibody is FITC, which tag to be choosed? GFP or RFP?
Thanks in advance!
-glcui-
GFP can not be used together with FITC, their fluorescence emission spectra are too similar. (you can compare spectra at http://www.bdbiosciences.com/spectra/). Don't know if retro or lentiviral would be better.
-vairus-
QUOTE (glcui @ Nov 17 2007, 01:54 AM)
Hi, everyone, I have to infect naive t cells with Gene X.
I get two choices: lentiviral or retroviral. Which one is better?
BTW, I am trying to generate a fusion protein of Gene X--GFP or RFP.
After infection, I 'd like to analyze these cells using FACS and hope to gate out GFP or RFP+ T cells and then see other cell markers in the gated cell population.
If another fluorescence of antibody is FITC, which tag to be choosed? GFP or RFP?
Thanks in advance!
I get two choices: lentiviral or retroviral. Which one is better?
BTW, I am trying to generate a fusion protein of Gene X--GFP or RFP.
After infection, I 'd like to analyze these cells using FACS and hope to gate out GFP or RFP+ T cells and then see other cell markers in the gated cell population.
If another fluorescence of antibody is FITC, which tag to be choosed? GFP or RFP?
Thanks in advance!
yeah, I will try RFP.
-glcui-