Has anyone transfected ST15A cells? - Transfection of ST15A cells? (Jul/13/2006 )
Hi,
Im trying to transfect siRNA into ST15A neuronal cells, but they massively die of the transfection. I have tried various reagents such as Lipofectamine 2000, RNAiMAx, Interferin?
Does anyone have any good suggestions; Im running out of ideas myself.
-Joo-
I infect shRNA into SHSY5Y cells (also neuronal) using lentivirus. U could also use AAV (adeno associated virus) to do the same.
-scolix-
QUOTE (Joo @ Jul 14 2006, 08:53 AM)
Hi,
Im trying to transfect siRNA into ST15A neuronal cells, but they massively die of the transfection. I have tried various reagents such as Lipofectamine 2000, RNAiMAx, Interferin?
Does anyone have any good suggestions; Im running out of ideas myself.
Im trying to transfect siRNA into ST15A neuronal cells, but they massively die of the transfection. I have tried various reagents such as Lipofectamine 2000, RNAiMAx, Interferin?
Does anyone have any good suggestions; Im running out of ideas myself.
I found that Oligofectamine (Invitrogen) doesn't cause cell death, you could try that one
-dnafactory-
My lab has been using gencarrier-2 forn neuronal lines like SY5Y, PC-12...Efficiency is highest and toxicity is lowest among other agents we tried.
-fair view-