Cell Proliferation inhibition - arrest? (May/19/2008 )

Hi,

I want to know what chemicals/methods I can use to stop proliferation of my adherent cell lines. I don't want them to proliferate anymore so I can do some experiments on them.

I searched biotech companies websites, but couldn't find any product or kit.

Thanks
Curtis

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you can try serum starvation method by using serum free medium for culture. Cells will survive for certain time.

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And will get synchronized too (in G0)

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ok thanks, that's what I thought actually....I am using 10% right now....can I drop the percentage to 1% immediately? or I have to adapt the cells to less serum first?

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I would suggest that you employ a sequential serum weaning procedure. E.g- 10% to 5% to 2.5% to 0.

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