determine cyp3a16 primer - mouse liver progenitor cell (Sep/25/2006 )

Hi everyone, I got problems of finding the suitable primer sets for the gene cyp3a16. My research topic is on investigating the expression of cyp3a16 and cyp3a11 on mouse liver progenitor cell before and after differentiation. However, for all of the journal articles I have go through, none of them perform PCR on cyp3a16 at all, I am just wondering is there a reason why we cant use PCR for determine the expression of cyp3a16. Thanks for everyone's help.

---

why not design your own?

I've worked extensively on the CYP3A subfamily and there is nothing unusual or troublesome about the region

---

I am just wondering is that possible for you to give me the primer sequence that you used for cyp3a11 and cyp3a16, so I can use this to double check whether my design is correct. This is simply because this is my first time doing a research project and I am just not feeling confident about what I am doing.

---

Hi there- What system are you using for RT PCR? If you are using Applied Biosystems, they have a pre-made gene expression assay for CYP3A16 (login may be required). I have found the gene expression assays sufficient for my needs, and they eliminate the need to design my own probes and primers.



If not, you may find this reference page useful (or not - I didn't look check it out myself).



Good luck!

---

I was working in human so don't have either of those

You might find this site useful though

http://drnelson.utmem.edu/CytochromeP450.html

---

Thanks, I have found out the primers that I am going to use, but got questions about the way to blast the primers in order to check for its specificity for cyp3a11 and cyp3a16 genes as I am looking for the gene expression in mouse liver cells. Each blast methods should I use in NCBI website, as there are so many types and I am getting confused about which one to use.

---