Topo TA cloning kit with Top 10 cells - Topo TA cloning kit with Top 10 cells (Dec/10/2006 )

Hi guys!

I use competent Top10 E.coli cells in my Topo Ta cloning kit. I wonder if i can incubate these competent cells over night at 35 celcius degrees instead for 37celcius degrees?
Will the cells grow well?

Thanks for your help!

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Yes, they will grow fine.

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But i guess they will not grow optimally as at 37C ?

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They will grow a little slower, probably not noticeably. Don't worry, be happy.

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Thanks alot!

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