Tris and cell toxicity - (Feb/14/2008 )

Hey guys,

I've read that Tris is a toxic polyamine, so i was just wondering what sort of an effect 10 mM Tris.HCl (common DNA buffer) would have on the toxicity of mammalian cells?

Cheers, Rob

-killerkoz17-

Er, that's not a good idea, I tried it once and my cells just died in minutes ... nothing as PBS it's way better. Good luck!

QUOTE (killerkoz17 @ Feb 14 2008, 07:56 PM)

Hey guys,

I've read that Tris is a toxic polyamine, so i was just wondering what sort of an effect 10 mM Tris.HCl (common DNA buffer) would have on the toxicity of mammalian cells?

Cheers, Rob

-aleruiz-

I can't say anything about animal cells, but for e coli you will see growth retardation at about 1mM concentration.

-perneseblue-

QUOTE (aleruiz @ Feb 16 2008, 11:41 AM)

Er, that's not a good idea, I tried it once and my cells just died in minutes

... nothing as PBS it's way better. Good luck!

What concentration killed your cells and what was the cell line aleruiz? I can't find anything on the net about this.

Cheers, Rob

-killerkoz17-

QUOTE (killerkoz17 @ Feb 17 2008, 05:39 PM)

QUOTE (aleruiz @ Feb 16 2008, 11:41 AM)

Er, that's not a good idea, I tried it once and my cells just died in minutes

... nothing as PBS it's way better. Good luck!

What concentration killed your cells and what was the cell line aleruiz? I can't find anything on the net about this.

Cheers, Rob

hi!

I can't remember the concentration... it was in mM range, maybe 10 mM (or 100?), but I can't remember and I'm not working in that lab anymore to check my notes

but the cells were primary mouse fibroblasts. When that happened, the tris-killed cells, a collegue in the lab showed me a note in the Sambrook's book (Molecular Biology) about Tris toxicity to cells, but just that as a literautre reference. Good luck!

-aleruiz-