PC12 cells do not attach on collagen - (Nov/26/2008 )

Hi,

my PC12 cells (transfected with VGlut2) do not attach to the cell surface when plated on collagen coated cover slips. Any suggestions how i can change that? I tried and tried. The grow happily in suspension ...

thanks,
Tine

Hi,

Are you using glass coverslips? Because PC12 cells do not grow well on glass AFAIK. We had the same problem and solved it by simply growing the cells on a plastic plate and cut the plate to microscope slide sized squares with a hot scalpel. But I think there are way more subtle ways to do this . You could try chamber slides or something similar.

Hope it helps

Hi,

thanks for your answer. Yes, i am using glass coverslips. Anyway, i was trying to grow them on plastic a while ago (just to see if they would settle down) but they are simply not sticking to the ground....
I´ll keep trying



quote name='DavidJ' date='Dec 1 2008, 06:59 PM' post='159079']
Hi,

Are you using glass coverslips? Because PC12 cells do not grow well on glass AFAIK. We had the same problem and solved it by simply growing the cells on a plastic plate and cut the plate to microscope slide sized squares with a hot scalpel. But I think there are way more subtle ways to do this . You could try chamber slides or something similar.

Hope it helps
[/quote]

Hi,
I have been growing PC12s on 96-well plates coated with poly-D-lysine (BD Biocoat). I compared their growth on these plates to uncoated Costar multiwell plates, and they MUCH prefer the poly-lysine to uncoated plastic.

Maybe you could coat your coverglass with poly-lysine.....it's relatively cheap.
Hope it helps!

That's surprising, I once grew them on Vitrogen ( a brand of collagen available from Invitrogen) and they grew just fine. Of course, every clonal varaint ofa cell line has different properties.......