transfecting adherent cells - before adherence? - (Dec/08/2006 )

Has anyone ever tried transfecting adherent cells, before they adhere? That is, I would like to transfect some HEK cells, but my plates are a little too confluent (about 80%: I prefer 50%). Is there a way I could trypsinize them, then add the transfection reagents, then plate them?
Thanks,
mito 1

-mito1-

QUOTE (mito1 @ Dec 8 2006, 06:53 PM)

Thanks,
mito 1

Yer, there is the retrotransfection method with lipofectamine or lipofectamine2000. It is on the datasheet. An alternative is to seed them in the morning, wait for 5 hours ca. and then transfect them.

-dnafactory-

Beauty!!
Thanks so much, dnafactory - you are a lifesaver!
mito 1

-mito1-

QUOTE (mito1 @ Dec 8 2006, 07:03 PM)

Beauty!!
Thanks so much, dnafactory - you are a lifesaver!

mito 1

I wouldn't exagerate... Thay haven't given me the red swimming suit of baywatch...

-dnafactory-

Just to follow-up...
The transfection seems to have worked like a charm
Thanks again, dnafactory!

-mito1-

QUOTE (mito1 @ Dec 14 2006, 05:42 PM)

Just to follow-up...
The transfection seems to have worked like a charm

Thanks again, dnafactory!

You are welcome!

-dnafactory-