preparation of electroporation competent cells from chemically competent cells - (Sep/17/2007 )

I currently have chemically compentent DH5alpha cells stored at -80. When these cells are streaked out and grown in LB they will no longer be chemically competent cells, can i then use these cells to prepare electrocompetent cells for electroporation?

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hi,
as long as I know and worked, chemically competent cells should be used imeediately, and they will lose their competency if stored, why do you wanna use those for making electroporation competent, don't you have stock? it's better to use them instead of competent resourse.
good luck

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Yes, you can. Once streaked out, just take the a colony off the plate, grow it up and put the culture through the protocol to make electrocompetent cells.

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Thank you both very much for your comments. It's good to know that I can do this (although it is now a theoretical problem as we have loacted the missing stock of electrocompetent cells....)

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