Why HepG2 and HUH7 - for hepatitis cell culture matter. (Aug/08/2006 )
From pfmdr
PS : my pm is not a subforum...
can u please tell me why HepG2 and Huh7 cells are used for hepatitis cell culture.only because they have receptors for HBV
And why duck glycine decarboxylase (DGD)expression is observed after post transfection.
i will be very much thankful.please as early as possible
God may bless.good by
Poor fred.
Thats because these are hepatocyte-derived. Acually, they are poor host cells for supporting HBV virus replication. Not sure what did you mean by DGD expression. Can you tell us the source of this work?
Thats because these are hepatocyte-derived. Acually, they are poor host cells for supporting HBV virus replication. Not sure what did you mean by DGD expression. Can you tell us the source of this work?
"Genome Replication, Virion Secretion, and e Antigen Expression of Naturally Occurring Hepatitis B Virus Core Promoter Mutants"
FIG. 3.
Transfection of 12 HBV genomes in Huh7 cells (A) and HepG2 cells (
. Cells grown in 10-cm dishes were transfected with 15 μg of HBV dimer and 15 μg of duck glycine decarboxylase (DGD) cDNA ......................................................................... If I understood correctly, DCD is included in transfection as an internal control to qunatitate/normalize RNA expression level. Transfection is a technique known to vary a lot in different wells/dishes due to minute details of experimental procedure are done slightly differently. It is always good to have an internal control to correct the variation introduced solely by transfection. DCD is from duck and will not have endogenous mRNA similar to human RNA, therefore can be specifically probed by Northern blot. You certainly do not have to use the same DCD for your experiment, as the authors used it because they have it on hand.