Trypsin/EDTA - how it works? (Jul/29/2007 )

Hello!

I´m trying to perform chemical acellularization in some pieces of tissue. In some research I have been done I found that Trypsin/EDTA is very usefull for that...
I need help! how does this thing works??? Can someone please explain that to me, post a web link with some information or send me something about it??

thanks!

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Hi

I'm surpised nobody answered but actually it might be because it's very vaste... Indeed the this thing, as you call it , usage depends a lot on the tissu you are working with, the size of your sample, etc.
The principal is that EDTA chelate (monopolise) every useful ions (such as Mg++, and Ca++) for binding molecules (integrins, fibronectin etc) and trypsin cuts ""everything" that emerge from out the cell" including binding molecules.
So the concentration and the time of incubation depends a lot on the tissu you're studying and the results you wanna get (if you care more about the state of your cells or the efficiency of your acellularization)

Good luck!

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