transfecting DNA into Jurkat cell - (Apr/04/2006 )

hi
I have to transfect DNA into Jurkat cell and do luciferase assay. I had transfect GFP into Jurkat and get efficiency <10% . now, I will do that again with bio-rad. Did someone do that?? Can you tell me your protocol or other detail step??? I also transfect with CMV plasmid but the luc just 1370......

cell numbers 8*10^5 20ug DNA, Eppendrof'S buffer and cuvette

THANKS~

Hi,

Use JetPEI for Jurkat cells it is the best, I compare it with Fugen "from roche" for pGL3 and pRL constructs transfection and it was about 10 times much better.
Good Luck

ABBADY