Isolation of cell membrane - (May/04/2001 )

Can anybody recommendate a good method for isolation of cell membrane from cell line? Thanks a lot. Yaoting

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YES. I AM WORKING ON SIGNALING PATHWAY VIA CELL MEM. AND THE METHOD WE ARE USING IS WORK WELL IN MY HAND.PLEASE LET ME KNOW YOUR EMAIL ADDRESS SO THAT I WILL LIKE TO SEND MY METHOD.

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Would this method also work for isolating cell surface/membrane associated antigens and if so, could you explain the procedure to me?

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Hi,Do you think your method will work also for partially purifying receptors from membrane?If so please forward it to me.Yael

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Would you please give me a protocol to prepare the bacterial membranes for the western blot. The membranes I prepared didn't dissolve properly in Tris buffer.

Thanks, Xueyan Duan

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Hi,
can you mail me the method too. haviong a tough time isolating membrane from cell line... because of quantity problems. Mine emal ID is garg_v@yahoo.com.
thanks

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