express toxic protein in insect cells - (Jul/08/2008 )
Hi all,
I'm going to express a glycosylated nuclease in insect cells. But I wonder if I could get any protein since the nuclease might kill the cells.
Had anyone had any experience with toxic protein expression?
Many many thanks.
-zzll-
QUOTE (zzll @ Jul 8 2008, 01:10 PM)
Hi all,
I'm going to express a glycosylated nuclease in insect cells. But I wonder if I could get any protein since the nuclease might kill the cells.
Had anyone had any experience with toxic protein expression?
Many many thanks.
I'm going to express a glycosylated nuclease in insect cells. But I wonder if I could get any protein since the nuclease might kill the cells.
Had anyone had any experience with toxic protein expression?
Many many thanks.
Check many of these links:
http://search.vadlo.com/b/q?sn=158621799&a...ssion&rel=0
..
-cellcounter-
QUOTE (cellcounter @ Jul 9 2008, 07:20 PM)
QUOTE (zzll @ Jul 8 2008, 01:10 PM)
Hi all,
I'm going to express a glycosylated nuclease in insect cells. But I wonder if I could get any protein since the nuclease might kill the cells.
Had anyone had any experience with toxic protein expression?
Many many thanks.
I'm going to express a glycosylated nuclease in insect cells. But I wonder if I could get any protein since the nuclease might kill the cells.
Had anyone had any experience with toxic protein expression?
Many many thanks.
Check many of these links:
http://search.vadlo.com/b/q?sn=158621799&a...ssion&rel=0
..
Thank you, cellcounter. I will check the links.
-zzll-