Re: how to seperate deadsuspension cells from alive ones - (Oct/03/2001 )

try using percol gradient centrifugation. i've forgotten the manufacturer. they do have protocols for separating live/dead cells. you will have to optimize the percol density(s) for your type of cells. you may also have to use more than one density gradient of percol to separate the layers of dead and live cells.good luck. john

use Ficoll from Amershan can also meet your target, the dead cell would be spinned down to the bottom