transformation of competent cells - (Jul/09/2007 )
Hi to all ofyou hanging there
I have question may be silly one could any body tell me for how many days i can still use the transformed competent cells i mean if u transform 50 ul of ccs with 2 ul of ligation mixture then u add 950 SOC medium etc....then u plate 50 to 100ul can i keep the rest in 4C (900ul if i plate 100ul) please if somebody have tried it reply me
-pCambia 1301-
QUOTE (pCambia 1301 @ Jul 9 2007, 02:41 AM)
Hi to all ofyou hanging there
I have question may be silly one could any body tell me for how many days i can still use the transformed competent cells i mean if u transform 50 ul of ccs with 2 ul of ligation mixture then u add 950 SOC medium etc....then u plate 50 to 100ul can i keep the rest in 4C (900ul if i plate 100ul) please if somebody have tried it reply me
I have question may be silly one could any body tell me for how many days i can still use the transformed competent cells i mean if u transform 50 ul of ccs with 2 ul of ligation mixture then u add 950 SOC medium etc....then u plate 50 to 100ul can i keep the rest in 4C (900ul if i plate 100ul) please if somebody have tried it reply me
Once I did leave this mixture at RT O/N and plated them the next day. I normally don't store them and usually spread the entire volume on to the plates.
No idea abt storing them in 4C.
-scolix-
I reccommed not storing more than O.N. There is a drastic decrease in transformation efficiency if you use >O.N strored transformed cells.
Hope this helps
-pras45-