Red Blood Cells exploding ! - (Nov/21/2005 )
Hello everyone,
did anyone hear of red blood cells exploding? mmm....me neither !!
but the fact is that I am making some experiments on the red blood cells through the micropipette aspiration technique. In this technique, cells are being absorbed through micropipettes (of few micrometers wide diameters, smaller than erythrocytes diameter) by a negative pressure. The medium used is the PBS buffer, the osmolarity is perfect, around 300 mOs. I tried different kinds of glas micropipettes, from borosilicate to aluminosilicate. The medium in the probe under the microscope and inside the micropipette is the same, the pressure system is very constant and as soon as a very small negative pressure is applied, the cell membrane will be destroyed, and therefore the cell will virtually ''explode'' and become a ghost cell.
Any idea or explanation? Help is appreciated !!
Cedarjet.
did anyone hear of red blood cells exploding? mmm....me neither !!
but the fact is that I am making some experiments on the red blood cells through the micropipette aspiration technique. In this technique, cells are being absorbed through micropipettes (of few micrometers wide diameters, smaller than erythrocytes diameter) by a negative pressure. The medium used is the PBS buffer, the osmolarity is perfect, around 300 mOs. I tried different kinds of glas micropipettes, from borosilicate to aluminosilicate. The medium in the probe under the microscope and inside the micropipette is the same, the pressure system is very constant and as soon as a very small negative pressure is applied, the cell membrane will be destroyed, and therefore the cell will virtually ''explode'' and become a ghost cell.
Any idea or explanation? Help is appreciated !!
Cedarjet.
did anyone hear of red blood cells exploding? mmm....me neither !!
but the fact is that I am making some experiments on the red blood cells through the micropipette aspiration technique. In this technique, cells are being absorbed through micropipettes (of few micrometers wide diameters, smaller than erythrocytes diameter) by a negative pressure. The medium used is the PBS buffer, the osmolarity is perfect, around 300 mOs. I tried different kinds of glas micropipettes, from borosilicate to aluminosilicate. The medium in the probe under the microscope and inside the micropipette is the same, the pressure system is very constant and as soon as a very small negative pressure is applied, the cell membrane will be destroyed, and therefore the cell will virtually ''explode'' and become a ghost cell.
Any idea or explanation? Help is appreciated !!
Cedarjet.
My sister-in-law is in the hospital on life support now because her red blood cells are exploding, causing her blood not to seperate, even when they spin it. The doctors are telling us that it is a mistory, that they have never seen anything like it. They say she might have been poisoned, but can't figure out with what. It happened imediatly after one of her dialisis sessions. They say she has less than a 20% chance of servival.
mommalou71