How to evaluate Cell viability - (Nov/08/2006 )
Hello Friends,
I am performing the MTT assay to determine the cell viability. I have done the experiment in 96 well plate format, with different drug concentration treatment with positive control and without treatment control.
I got the different OD s from spectrophotometry. Could you please tell me , that how could I get I get the viablity percentage from this assay?
Thanks
Deb
-debarshir-
QUOTE (debarshir @ Nov 8 2006, 06:27 PM)
Hello Friends,
I am performing the MTT assay to determine the cell viability. I have done the experiment in 96 well plate format, with different drug concentration treatment with positive control and without treatment control.
I got the different OD s from spectrophotometry. Could you please tell me , that how could I get I get the viablity percentage from this assay?
Thanks
Deb
I am performing the MTT assay to determine the cell viability. I have done the experiment in 96 well plate format, with different drug concentration treatment with positive control and without treatment control.
I got the different OD s from spectrophotometry. Could you please tell me , that how could I get I get the viablity percentage from this assay?
Thanks
Deb
relative to untreated control in percent
-The Bearer-