protein isolation from cell lines - membrane bound proteins (Dec/23/2007 )

hello every one,
i need to isolate membrane bound proteins from animal cells (using cancer cell line grown in lab condition)is any body have procedrue -how to isolate membrane bound proteins from cell lines.

thanks in advance,
nishanth

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What kind of membranes are you interested ? membrane-proteins in general or membranes of special organelles ? differential centrifugation should be the method of choice...

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...in combination with sucrose gradients...

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Hi, I'm interested to extract a transmembrane protein from a specific organelle in the cell line. What protocol can I use?

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