Maintaining cells without freezing - (Jul/25/2006 )

hello everybody...
I'd like to know if it's possible maintaining cells without freezing (i.e. at 4°C, in medium or in PBS)...That's because I can't freeze cells now but I leave for about 3 weeks and I'd like not to lose my cells.
thanks

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Best would b to freeze (-80C) and thaw them after u return.

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I would agree with that. You can freeze them (slowly and in freezing media i.e. DMSO+Media) in a -80C freezer and then thaw them when you get back. That way your cells will remain viable. I don't think they would last at all in a 4C.

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can someone deal with your cells during 3 weeks? replacing medium and eventually passage them is not that hard...

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I'd thought it but unfortunately, noone can do that for me...so I think that I'll freeze them

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So thanks everybody...my cells will sleep in liquid N (so they will be cold, it's so hot here)

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