HCT 116 colon carcinoma cells and colon cancer cells in general - feeder layer to increase plating efficiency? (Jan/11/2007 )
Hi,
I want to try an experiment using colon cacner cells because my treatment induces apoptosis in breast and ovarian cancer cells and would like to see if it would work with colon cells. Picked HCT116 cells b/c I found some papers using them. They grow very fast which is great, but they don'[t attach well, ie there are alot of floaters and we a reworrying that will effect our experiemnts. I can't find it now (its not on the ATCC product sheet) but somewhere I read that they require a feeder layer of fibroblasts I think? What is this? Has anyone grown these cells? Is there an easier colon cancer cell line to work with? Thanks for any thoughts.
You may need to get a new batch from ATCC or other sources. They should not behave like that.
No it doesnt require feeders cells and we juts culture them in DMEM+10% FBS
I want to try an experiment using colon cacner cells because my treatment induces apoptosis in breast and ovarian cancer cells and would like to see if it would work with colon cells. Picked HCT116 cells b/c I found some papers using them. They grow very fast which is great, but they don'[t attach well, ie there are alot of floaters and we a reworrying that will effect our experiemnts. I can't find it now (its not on the ATCC product sheet) but somewhere I read that they require a feeder layer of fibroblasts I think? What is this? Has anyone grown these cells? Is there an easier colon cancer cell line to work with? Thanks for any thoughts.
often used colon carcinoma cell lines are Caco-2 and T84 cells; Caco-2 is especially used to establish an epithelium, or lets say better an epithelium-like ayer of immortal cells;
if one decides for a special cell line, it carries unique features which may not be found in other cell lines; so, what will be the advantage of HCT116 cells? if co-culture with fibroblasts is necessary it does not sound very friendly for routine usage...