Storage of different cells - (Nov/07/2006 )

Hello people,

I'm having problems with the next question :

How are bacteria strains stored for a short period and how are they stored for a long period?

I think the short period would be storing them in their medium at 4 degrees celsius and for a longer period freeze dry them.
But the next question is the same as the first but now for fungi and eukaryotic cells. I think the short period should be the same as for the bacteria, but for the longer period i have no idea, maybe liquid nitrogen?

Any information would be great.

Thanks in advance!
Klebsy

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eukaryotic cells are stored in liquid nitrogen for longer periods but i don't know about fungi

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Long term storage of bacteria is as glycerol stocks at -80 degrees celcius (in about 20% glycerol/media)

Shorter term storage is either as streaks on an agar plate or stab or streak on a slant (at 4 degress celcius)

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Hi

Eukaryotic cells are stored in a mixture of DMSO and serum with some growth medium and then frozen in liquid nitrogen.

Funig can be stored as spores as far as I know, but I have never worked with them, so I am not sure.

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with fungi the best way to do it is to grow them on agar till you have lots of spores/mycelium and then scrape them off and store in 30% glycerol at -80 or resuspend the spore/mycelium mix in sterile skimmed milk and freeze dry for longer term storage

hope this helps

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great guys, thanks! really helpfull

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we preserve fungal spore suspension in 10% glycerol

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in -80c

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