growth curve? - how to establish growth curve of specific cell line.... (Sep/23/2008 )

hi

can any one help me how can i find or generate good growth curve of a specific cell line??????how to find cell line exponential phase????also tell me calculation with protocol

im confused


THANKS

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I take it you know how to count cells?

So, seed a several wells of a plate with cells at a known density (try 10,000 cells/cm², and then lift and count the number of cells per volume of harvest over a set time course (e.g. every 2 days harvest a well), work out how many cells you have in total and plot those numbers on a time vs cell number graph. Exponential phase is where cell numbers are doubling or more per unit time, plotting on a log-linear scale will help identify it as it will become straight rather than curved.

Calculation for what? If for the curve, try some regression analysis, or get a graphing program (not Excel, it is terrible) and use that to tell you.

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