cell lysis - (Nov/13/2008 )
Can the cell pellet be frozen for upto a week before lysis with ripa buffer to collect proteins for western blotting
-missi-
Yes, so long as it is frozen you can store it almost indefinitely. It is best to snap freeze (dry ice/methanol bath) cell pellets.
-bob1-
QUOTE (missi @ Nov 13 2008, 02:31 PM)
Can the cell pellet be frozen for upto a week before lysis with ripa buffer to collect proteins for western blotting
keep at -80 and there should be no problem.
-Curtis-
QUOTE (bob1 @ Nov 13 2008, 04:45 PM)
Yes, so long as it is frozen you can store it almost indefinitely. It is best to snap freeze (dry ice/methanol bath) cell pellets.
Ditto !!!!!!!! Great answer
-Rhombus-