Assay for cell purity determination - (Mar/06/2007 )
Currently employing flow cytometry to account for cell purity (Ab(x) versus Ab(y)). Can anyone provide another assay (aside from Western) to determine the purity of a cell suspension? Thank you for any insight.
-biop-
QUOTE (biop @ Mar 6 2007, 02:35 PM)
Currently employing flow cytometry to account for cell purity (Ab(x) versus Ab(y)). Can anyone provide another assay (aside from Western) to determine the purity of a cell suspension? Thank you for any insight.
immuncytochemistry and fluorescence microscopic inspection but more difficult to quantify
-The Bearer-
QUOTE (The Bearer @ Mar 6 2007, 04:29 PM)
QUOTE (biop @ Mar 6 2007, 02:35 PM)
Currently employing flow cytometry to account for cell purity (Ab(x) versus Ab(y)). Can anyone provide another assay (aside from Western) to determine the purity of a cell suspension? Thank you for any insight.
immuncytochemistry and fluorescence microscopic inspection but more difficult to quantify
Thanks for the suggestions, Bearer. As you mentioned, however, these methods will prove difficult to quantify- points taken.
-biop-