cell migration assay - (Dec/13/2008 )

Can you help there

I am doing cell migration assay using micro glia cells.
Migrated cells are labeled with Calcein.

1, can I use ABI 7300 to read the plates or I have to use a fluorescent plate reader?
2, which company sell phenol red-free MEM?
3, Does CV at 10-20% acceptable for cell migration assay?
Thanks very much

Percey

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If you can't find it in liquid form, maybe you should look for powdered MEM?
(As for 3 - what is "CV"? )

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coefficient of variance (CV = SD/Mean))

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