CHO cells medium - (May/19/2008 )

Hello evrybody! I'm looking for the components of a very good medium for CHO celll line... There are a lot of different mediums in verious articles, so what's your medium composition? Thanks!

-lory-

QUOTE (lory @ May 19 2008, 07:14 AM)

Hello evrybody! I'm looking for the components of a very good medium for CHO celll line... There are a lot of different mediums in verious articles, so what's your medium composition? Thanks!

We use F-12 plus 10 % FBS.

-genehunter-1-

QUOTE (lory @ May 19 2008, 06:14 AM)

Hello evrybody! I'm looking for the components of a very good medium for CHO celll line... There are a lot of different mediums in verious articles, so what's your medium composition? Thanks!

Look up may answers here:
http://search.vadlo.com/b/q?p=1&sn=158...rel=0&srt=0
..

-cellcounter-

I use Ham's F12K for my CHO-K1 cell line. I have also seen multiple medias used for CHO and one post-doc in my lab uses DMEM for his CHO cells. I went with the ATCC media suggestion since we got our cells from them. Honestly, I think CHO cells could grow in just about anything though.

-rkay447-

QUOTE (rkay447 @ May 19 2008, 12:39 PM)

on my hands, I dont think the cells like DMEM at all, RPMI-1640 maybe OK.

-genehunter-1-

Thank you for the suggestions!! i thnk i will use IMDM with 10% FCS and 1% L-Glu.... what do you think?

-lory-