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treat cells with progesterone and estrogen - (Sep/15/2006 )

Does anyone have any experience with treating epithelial cells with hormones? I just would like to know the finial concentratoin you used and the length of incubation. Thanks a lot.

-angelaileen-

QUOTE (angelaileen @ Sep 16 2006, 10:58 AM)
Does anyone have any experience with treating epithelial cells with hormones? I just would like to know the finial concentratoin you used and the length of incubation. Thanks a lot.


I treat MCF-7 cells with 2nM estradiol for 48 hours - I look at p53 activation. Hope this helps

-Gomer-