How many cells (nuclei) per ml pellet? - (Sep/08/2005 )

I asked this in some other forum before but had no luck.

If you have 1 ml pellet of, say, HeLa cell, how many cells (nuclei) are in there?
I just need a ballpark estimate...it doesn't even have to be HeLa cell.

-namekuji-

A full 15-cm dish human diploid skin fibroblasts contains approximately 1-2 million cells. The same full plate with HeLa cells van go up to 10 million cells. How many plates do you need to get 1 ml cell pellet?

-Theo22-

Thank you for the reply.

>How many plates do you need to get 1 ml cell pellet?
Actually, I don't know. I don't even work with cell cultures.
I have 1ml of tissue collected from live animals and I'm trying to figure out approximately how many cells there are.

So let me rephrase the question for you: if you pellet the fibroblast (or HeLa) fully growing in a 15 cm dish, how much volume would it be?

-namekuji-

QUOTE (namekuji @ Sep 9 2005, 12:22 AM)

So let me rephrase the question for you: if you pellet the fibroblast (or HeLa) fully growing in a 15 cm dish, how much volume would it be?

50-100 microliter? However I don't think you can compare this with tissues from live animals. So lets hope that someboby working with tissues as well will answer your question.

-Theo22-