Transfection of siRNA in suspension cells - (Mar/19/2008 )

Hi, has anyone done siRNA transfection in suspension cells (Jurkat A3 cells)?
Also, which transfection reagent would you recommend for HEK 293 cells?

Thanks.

---

When i tried transfecting in suspension , i had more than 50% cells die so i don't know how good it is .

---

Hek293 should be possible with different reagents.
I would recommend HiPerFect from Qiagen.
They further offer protocols for suspension cells (maybe even for Jurkat cells).
I consider it to be one of the best reagents I have worked with.

Check their website for more infos.

---

I did some transfection with Lipo2000 a few years ago and it was hard
I got like 10-20% efficiency and end up needing to select for stably transfected cells.

Transient transfection of suspension cells is no easy job

---

I used Lipofectamine2000 to transfect HEK293 cells with siRNA for few years ago and it was quite efficient. Are you sure that your cells were okay?

For Jurkat cells, if I am not wrong you could try electroporation (Amaxa system).

---

try electroporation if you have one avaiable. suspension cells are tough to get siRNA in with most chemical methods.

---

sorry, I mean I used another cell line not HEK293, but also suspension cells.

Most adherent cells I transfected are very good, that's why I have an impression that transfection with suspension cell using lipo method is not easy

---