How to tell cell are contaminated by fungi - (Aug/11/2006 )

I have a culture that I think might be contaminated with yeast, however I am not sure of it. There are round cells with occasional budding. The replication is very slow and the the media is not cloudy. Does anyone know how to make sure if there is yeast growing in my culture? Any help would be greatly appriciated. Thanks.

-maak34-

As u wrote that cells are budding, I would assume its contaminated. Better to show someone in the lab to confirm and dispose cells immediately.

-scolix-

Scolix, what happen if you have transfected your cells for the production of adenovirus and these cells get contaminated with yeast?, the adenovirus production is been affected??? what happen with the possible adenovirus????

QUOTE (scolix @ Aug 11 2006, 09:24 PM)

As u wrote that cells are budding, I would assume its contaminated. Better to show someone in the lab to confirm and dispose cells immediately.

-MCR-

I would not harvest adenovirus if cell culture is contaminated. I am not sure abt the adenovirus produced by these cells. But I would assume that adenovirus production could b decreased.

-scolix-

but, could be any change or damage in the adenovirus it self because the yeast.

QUOTE (scolix @ Aug 26 2006, 09:09 PM)

I would not harvest adenovirus if cell culture is contaminated. I am not sure abt the adenovirus produced by these cells. But I would assume that adenovirus production could b decreased.

-MCR-