Cell passage number for non-cancerous cells - (Sep/11/2007 )
I have two questions:
1. What is the recommended number of passages for non-cancerous cells in general? What about specifically Murine macrophage cell line (RAW cells)? Or Human gingival fibroblasts (HGF-1) ?
2. Has anyone heard of the idea that when you freeze cells at a certain passage number that the freezing turns back the clock to zero? If not if I were to freeze cells at passage 3 for example, when I defrost them, is the passage number considered 4?
Thanks in advance for all your help!
each cell line can be used for a certain number of passages. I am not familiar with the cells you are using. You could ask the person or company which provided the cells.
IF you freeze cells at passage 3 and thaw them, they are not at passage 1 but at passage 4.
1. What is the recommended number of passages for non-cancerous cells in general? What about specifically Murine macrophage cell line (RAW cells)? Or Human gingival fibroblasts (HGF-1) ?
2. Has anyone heard of the idea that when you freeze cells at a certain passage number that the freezing turns back the clock to zero? If not if I were to freeze cells at passage 3 for example, when I defrost them, is the passage number considered 4?
Thanks in advance for all your help!
Dear izun,
If they are RAW 267.4 then you can passage them 200-300 times without a problem. We have used them and J774.A1 in our Nitric Oxide work with no problems. They are in fact the easiest cells to grow.
As for the frozen status of cells, scolix is spot on, P3 cells frozen are P4 cells when they come back out of nitrogen.
Transformed/immortalised cells in general can be passaged more times than PRIMARY cells that you isolate.
Hope this is useful
Rhombus