why my suspension cells changed to adherent cells? - (Oct/03/2007 )
In our lab, 2 kinds of suspesnsion cells have changed to adherent cells, which did affect our experiments. I thought it is because they cultured suspension cells in treated flasks that are for adherent cells.
so after I came to this lab, I use non-coated flasks specially for suspension cells. but still after sometime, these suspension cells became adherent, without any noticable mistake during culture.
so can someone here teach me what happened on earth and how to avoid this??
-aniu-
QUOTE (aniu @ Oct 3 2007, 02:08 AM)
In our lab, 2 kinds of suspesnsion cells have changed to adherent cells, which did affect our experiments. I thought it is because they cultured suspension cells in treated flasks that are for adherent cells.
so after I came to this lab, I use non-coated flasks specially for suspension cells. but still after sometime, these suspension cells became adherent, without any noticable mistake during culture.
so can someone here teach me what happened on earth and how to avoid this??
so after I came to this lab, I use non-coated flasks specially for suspension cells. but still after sometime, these suspension cells became adherent, without any noticable mistake during culture.
so can someone here teach me what happened on earth and how to avoid this??
as your type/line of cells is not offered, it is only to discuss in general; some cells can grow in suspension but can also attach at appropriate matrix; or your culture condition differentiate cells from suspension to attaching type;
what will happen if you offer a non-attachment matrix such as polyacrylate?? will cells remain suspended?
-The Bearer-
QUOTE (The Bearer @ Oct 3 2007, 06:15 PM)
QUOTE (aniu @ Oct 3 2007, 02:08 AM)
In our lab, 2 kinds of suspesnsion cells have changed to adherent cells, which did affect our experiments. I thought it is because they cultured suspension cells in treated flasks that are for adherent cells.
so after I came to this lab, I use non-coated flasks specially for suspension cells. but still after sometime, these suspension cells became adherent, without any noticable mistake during culture.
so can someone here teach me what happened on earth and how to avoid this??
so after I came to this lab, I use non-coated flasks specially for suspension cells. but still after sometime, these suspension cells became adherent, without any noticable mistake during culture.
so can someone here teach me what happened on earth and how to avoid this??
as your type/line of cells is not offered, it is only to discuss in general; some cells can grow in suspension but can also attach at appropriate matrix; or your culture condition differentiate cells from suspension to attaching type;
what will happen if you offer a non-attachment matrix such as polyacrylate?? will cells remain suspended?
thank you for your reply, my cell line is RMA-S, a lymphoma cell line. do you by chance know the detail of this cell line?
-aniu-
QUOTE (aniu @ Oct 4 2007, 08:21 PM)
QUOTE (The Bearer @ Oct 3 2007, 06:15 PM)
QUOTE (aniu @ Oct 3 2007, 02:08 AM)
In our lab, 2 kinds of suspesnsion cells have changed to adherent cells, which did affect our experiments. I thought it is because they cultured suspension cells in treated flasks that are for adherent cells.
so after I came to this lab, I use non-coated flasks specially for suspension cells. but still after sometime, these suspension cells became adherent, without any noticable mistake during culture.
so can someone here teach me what happened on earth and how to avoid this??
so after I came to this lab, I use non-coated flasks specially for suspension cells. but still after sometime, these suspension cells became adherent, without any noticable mistake during culture.
so can someone here teach me what happened on earth and how to avoid this??
as your type/line of cells is not offered, it is only to discuss in general; some cells can grow in suspension but can also attach at appropriate matrix; or your culture condition differentiate cells from suspension to attaching type;
what will happen if you offer a non-attachment matrix such as polyacrylate?? will cells remain suspended?
thank you for your reply, my cell line is RMA-S, a lymphoma cell line. do you by chance know the detail of this cell line?
unfortunately not
but try another post with the name of the cell line in the title to appeal more readers...
-The Bearer-