breast cancer cell primary culture - (Dec/19/2004 )

Hi
Is there anyone has experience on the primary culture of breast cancer? could you provide the protocol how to set up this kind of primary culture? Thank you very much
steven

I used to do breast primary culture. I'll see if I can find the protocol tonight amoung my old backups or in Electronic copies of my students thesis. Otherwise our group published it in:

Speirs V., Green A. R., Walton D. S., Kerin M. J., Carleton P. J., Fox J. N., Desai S. B. and Atkin S. L., Short-term primary culture of epithelial cells derived from human breast tumours. Br. J. Cancer,78(11):1421-9 1998

V. Speirs, A. R. Green and M. C. White, Collagenase III: a superior enzyme for complete disaggregation and improved viability of normal and malignant breast tissue. In Vitro Cell. Dev. Biol. 32 (1996), pp. 72–74.

J. T. Emerman and D. A. Wilkinson, Routine culturing of normal, dysplastic and malignant human mammary epithelial cells from small tissue samples. In Vitro Cell. Dev. Biol. 26 (1990), pp. 1186–1194.

I've just checked and none of these seem to be online. can you get them from a library?

Either I could email you our method or a similar method can be found in Pubmed paper

good luck


Ian