stimulation of cell division/mitosis? - of non-dividing exocrine cells (Feb/16/2008 )

we have isolated a type of exorine epithelial cells which have a natural short life and normally do not divide;

yet, what could be tried to trigger mitosis?

-The Bearer-

QUOTE (The Bearer @ Feb 16 2008, 01:55 PM)

we have isolated a type of exorine epithelial cells which have a natural short life and normally do not divide;

yet, what could be tried to trigger mitosis?

Hi,
In this paper, they used TERT and sv40 LT for primary human BJ fibroblasts.
Nature 428, 431-437 (25 March 2004)

-WHR-

QUOTE (WHR @ Feb 17 2008, 12:29 AM)

QUOTE (The Bearer @ Feb 16 2008, 01:55 PM)

we have isolated a type of exorine epithelial cells which have a natural short life and normally do not divide;

yet, what could be tried to trigger mitosis?

Hi,
In this paper, they used TERT and sv40 LT for primary human BJ fibroblasts.
Nature 428, 431-437 (25 March 2004)

without having read the paper, isn´t it just for immortalization?

-The Bearer-

QUOTE (The Bearer @ Feb 17 2008, 12:38 AM)

[without having read the paper, isn´t it just for immortalization?

Bj cell proliferation arrest is p53 dependent. so they used LT to inactivate p53.

-WHR-

QUOTE (WHR @ Feb 17 2008, 03:25 AM)

QUOTE (The Bearer @ Feb 17 2008, 12:38 AM)

[without having read the paper, isn´t it just for immortalization?

Bj cell proliferation arrest is p53 dependent. so they used LT to inactivate p53.

I see, thank you

-The Bearer-