Primary tracheal cell culture - (Jul/08/2008 )
Hi,
I am trying to prepare primary tracheal cell culture from chick. I tried to dissociate the epithelial cells from trachea sections with 0.2% type XIV protease from Streptomyces griseus with Pen/Strep antibiotics in DMEM for 4hrs in 37C incubator with rotation. However, I got very few cells. I am wondering if there is other things I need to put into the dissociation solution.
Thanks!
-greenlime-
QUOTE (greenlime @ Jul 8 2008, 11:19 AM)
Hi,
I am trying to prepare primary tracheal cell culture from chick. I tried to dissociate the epithelial cells from trachea sections with 0.2% type XIV protease from Streptomyces griseus with Pen/Strep antibiotics in DMEM for 4hrs in 37C incubator with rotation. However, I got very few cells. I am wondering if there is other things I need to put into the dissociation solution.
Thanks!
I am trying to prepare primary tracheal cell culture from chick. I tried to dissociate the epithelial cells from trachea sections with 0.2% type XIV protease from Streptomyces griseus with Pen/Strep antibiotics in DMEM for 4hrs in 37C incubator with rotation. However, I got very few cells. I am wondering if there is other things I need to put into the dissociation solution.
Thanks!
If this is kind of similar in mice and chick (which it may not be), you can use one of these links:
http://search.vadlo.com/b/q?sn=158621799&a...ture+&rel=0
..
-cellcounter-