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cell culture - (Sep/16/2008 )

Dear all,
I have K562 cell lines maintained in RPMI medium.How to separate the dead cells from the live cells?Upon spinning at a low speed they are remaining.
Anjan

-anjan-

QUOTE (anjan @ Sep 16 2008, 02:18 AM)
Dear all,
I have K562 cell lines maintained in RPMI medium.How to separate the dead cells from the live cells?Upon spinning at a low speed they are remaining.
Anjan


Just change media in the flask and the floating dead cells will be sucked out and the live cells will remain.

-scolix-