cell cryopreservation in -80 - (Aug/18/2008 )
hi, there will have any consequences on my peripheral blood mononuclear cells if i transfert them from nitrogen liquid to -80.
please help me because liquid nitrogen is very expensive for my laboratory
thanks for helps
-tregis6-
QUOTE (tregis6 @ Aug 18 2008, 12:44 AM)
hi, there will have any consequences on my peripheral blood mononuclear cells if i transfert them from nitrogen liquid to -80.
please help me because liquid nitrogen is very expensive for my laboratory
thanks for helps
please help me because liquid nitrogen is very expensive for my laboratory



thanks for helps
I've been keeping my cells in -80 for 6 months now, when I re-grow them they grow very fine. I have no problem with that. my friend keeps them in -20 for 2 months, also he has no problem.
but I'm not sure for transferring from liquid nitrogen to -80, this is a big temperature change.
-Curtis-
well the ideal would be to thaw your cells, culture them 1 -2 passage and freeze the culture at-80°.
The heat shock may be too strong.
-fred_33-