Thp-1 cell transduction - (Jun/22/2007 )

Hi
I'm trying to transduce THP-1 cells using lentivirus. Does anyone have experience of transducing THP-1 cells I'd gladly accept any tips or advice on the topic
thanks

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Hi Susie,

If you're trying to transduce THP-1 cells using lentivirus, I advise you to use ViroMag R/L, which is supplied by OZBiosciences. ViroMag R/L is a magnetic beads containing formulation specifically designed for retrovirus and lentivirus, which allows to increase your transduction efficiency. Actually, you just have to mix ViroMag R/L and virus to be transduce and then vectors and viruses associated with magnetic particles are driven into target cells using a magnetic fields. It works very well.

cec.

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cec44: Are you sure that you need magnetic force to drive lentivirus into cells?

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Actually, several papers show a significant increase of transduction efficiency by using magnetic force to drive viruses & vectors in association with magnetic particles into cells. Personnaly, I use Magnetofection to tranduce my cells because I obtain better results with it.

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choose promoters in the lentiviral vectors which have been proven to work in these cells.

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